Genetic and Molecular Regulation of Seed Storage Proteins (SSPs) to Improve Protein Nutritional Value of Oilseed Rape (Brassica napus L.) Seeds

Gacek, Katarzyna; Bartkowiak-Broda, I.; Batley, Jacqueline

doi:10.3389/fpls.2018.00890

Cited by 41 publications

(40 citation statements)

References 77 publications

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“…There is increasing effort to improve the nutritional value of B. napus meal in order to overcome the dependence on the expensive soybean imports in regions where soybean cannot be grown (Jasinski et al, 2018). So far, rapeseed meal is used for aquatic and poultry industries as a feed, but it can also be used as a valuable protein source for humans and non-ruminants (Gacek et al, 2018). However, the presence of various anti-nutritive compounds like phytic acid, sinapine, fibre and glucosinolates impedes its commercialization as a major protein source.…”

Section: Discussionmentioning

confidence: 99%

Gene editing of three BnITPK genes in tetraploid oilseed rape leads to significant reduction of phytic acid in seeds

Sashidhar

Harloff

Potgieter

et al. 2020

Plant Biotechnology Journal

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Commercialization of Brassica napus. L (oilseed rape) meal as protein diet is gaining more attention due to its well-balanced amino acid and protein contents. Phytic acid (PA) is a major source of phosphorus in plants but is considered as anti-nutritive for monogastric animals including humans due to its adverse effects on essential mineral absorption. The undigested PA causes eutrophication, which potentially threatens aquatic life. PA accounts to 2-5% in mature seeds of oilseed rape and is synthesized by complex pathways involving multiple enzymes. Breeding polyploids for recessive traits is challenging as gene functions are encoded by several paralogs. Gene redundancy often requires to knock out several gene copies to study their underlying effects. Therefore, we adopted CRISPR-Cas9 mutagenesis to knock out three functional paralogs of BnITPK. We obtained low PA mutants with an increase of free phosphorus in the canola grade spring cultivar Haydn. These mutants could mark an important milestone in rapeseed breeding with an increase in protein value and no adverse effects on oil contents.

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Section: Discussionmentioning

confidence: 99%

Gene editing of three BnITPK genes in tetraploid oilseed rape leads to significant reduction of phytic acid in seeds

Sashidhar

Harloff

Potgieter

et al. 2020

Plant Biotechnology Journal

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“…The model species Arabidopsis thaliana L. has played a key role in identifying gene regulatory networks that govern seed development and germination. A wide repertoire of genetic technologies enabled the identification of essential regulatory genes during seed development and germination in Arabidopsis as well as the identification of orthologous genes in other plant species [ 15 , 16 , 17 , 18 , 19 , 20 ]. These technologies include forward genetic screens of lines obtained by T-DNA insertional mutagenesis for tagged mutants that produce a knockout phenotype, microarray RNA transcriptional profiling, and identification of seed-specific transcription factors (TFs).…”

Section: Introductionmentioning

confidence: 99%

“…These technologies include forward genetic screens of lines obtained by T-DNA insertional mutagenesis for tagged mutants that produce a knockout phenotype, microarray RNA transcriptional profiling, and identification of seed-specific transcription factors (TFs). Genes involved in the regulatory networks responsible for the synthesis, accumulation and mobilization of seed storage proteins have been identified in Arabidopsis and other plants [ 20 , 21 ]. Dormancy induction and germination are greatly regulated by the dynamic balance between the functional antagonist abscisic acid (ABA) and gibberelic acid (GA) phytohormones [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Advances in the Biology of Seed and Vegetative Storage Proteins Based on Two-Dimensional Electrophoresis Coupled to Mass Spectrometry

et al. 2018

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Seed storage proteins play a fundamental role in plant reproduction and human nutrition. They accumulate during seed development as reserve material for germination and seedling growth and are a major source of dietary protein for human consumption. Storage proteins encompass multiple isoforms encoded by multi-gene families that undergo abundant glycosylations and phosphorylations. Two-dimensional electrophoresis (2-DE) is a proteomic tool especially suitable for the characterization of storage proteins because of their peculiar characteristics. In particular, storage proteins are soluble multimeric proteins highly represented in the seed proteome that contain polypeptides of molecular mass between 10 and 130 kDa. In addition, high-resolution profiles can be achieved by applying targeted 2-DE protocols. 2-DE coupled with mass spectrometry (MS) has traditionally been the methodology of choice in numerous studies on the biology of storage proteins in a wide diversity of plants. 2-DE-based reference maps have decisively contributed to the current state of our knowledge about storage proteins in multiple key aspects, including identification of isoforms and quantification of their relative abundance, identification of phosphorylated isoforms and assessment of their phosphorylation status, and dynamic changes of isoforms during seed development and germination both qualitatively and quantitatively. These advances have translated into relevant information about meaningful traits in seed breeding such as protein quality, longevity, gluten and allergen content, stress response and antifungal, antibacterial, and insect susceptibility. This review addresses progress on the biology of storage proteins and application areas in seed breeding using 2-DE-based maps.

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“…Brassica napus is an important oilseed crop, ranking as the second most important crop for oilseed production worldwide [1], and it was derived from the hybridization between B. rapa and B. oleracea [2]. To satisfy the increasing demand for oil, it is essential to improve various important economically and agriculturally traits via genetic engineering techniques, which are powerful tools for gene functional analysis and crop improvement [3].…”

Section: Introductionmentioning

confidence: 99%

A convenient, rapid and efficient method for establishing transgenic lines of Brassica napus

Zhang

Liu

et al. 2020

Plant Methods

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Background: Brassica napus is an important oilseed crop that offers a considerable amount of biomass for global vegetable oil production. The establishment of an efficient genetic transformation system with a convenient transgenic-positive screening method is of great importance for gene functional analysis and molecular breeding. However, to our knowledge, there are few of the aforementioned systems available for efficient application in B. napus. Results: Based on the well-established genetic transformation system in B. napus, five vectors carrying the red fluorescence protein encoding gene from Discosoma sp. (DsRed) were constructed and integrated into rapeseed via Agrobacterium-mediated hypocotyl transformation. An average of 59.1% tissues were marked with red fluorescence by the visual screening method in tissue culture medium, 96.1% of which, on average, were amplified with the objective genes from eight different rapeseed varieties. In addition, the final transgenic-positive efficiency of the rooted plantlets reached up to 90.7% from red fluorescence marked tissues, which was much higher than that in previous reports. Additionally, visual screening could be applicable to seedlings via integration of DsRed, including seed coats, roots, hypocotyls and cotyledons during seed germination. These results indicate that the highly efficient genetic transformation system combined with the transgenic-positive visual screening method helps to conveniently and efficiently obtain transgenic-positive rapeseed plantlets. Conclusion: A rapid, convenient and highly efficient method was developed to obtain transgenic plants, which can help to obtain the largest proportion of transgene-positive regenerated plantlets, thereby avoiding a long period of plant regeneration. The results of this study will benefit gene functional studies especially in high-throughput molecular biology research.

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Genetic and Molecular Regulation of Seed Storage Proteins (SSPs) to Improve Protein Nutritional Value of Oilseed Rape (Brassica napus L.) Seeds

Cited by 41 publications

References 77 publications

Gene editing of three BnITPK genes in tetraploid oilseed rape leads to significant reduction of phytic acid in seeds

Gene editing of three BnITPK genes in tetraploid oilseed rape leads to significant reduction of phytic acid in seeds

Advances in the Biology of Seed and Vegetative Storage Proteins Based on Two-Dimensional Electrophoresis Coupled to Mass Spectrometry

A convenient, rapid and efficient method for establishing transgenic lines of Brassica napus

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