Genetic and molecular analysis of the restoration of fertility (Rf) genes for Ogura male-sterility from a Japanese wild radish (Raphanus
                     sativus var. hortensis f. raphanistroides Makino)

Yasumoto, Keita; Nagashima, Takaharu; Umeda, Tetsuya; Yoshimi, M.; Yamagishi, Hiroshi; Terachi, Toru

doi:10.1007/s10681-008-9687-4

Cited by 7 publications

(4 citation statements)

References 11 publications

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“…Several CMS-Rf systems have been characterized to date in many important crops, including the CMSRf 2 in maize (Cui et al 1996;Liu et al 2001), CMS-Rf in petunia (Bentolila et al 2002), and CMS-Rfo in radish (Brown et al 2003;Koizuka et al 2003;Yasumoto et al 2008b). Unlike the Rf 2 gene, which reduces the amount of a toxic aldehyde present as a result of residual URF13 expression (Liu and Schnable 2002), the restorer genes in petunia and radish encode a protein containing multiple copies of pentatricopeptide repeat (PPR) motif that is known to affect either the transcript profile or the protein accumulation of the CMS-associated gene.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of the Rs-Rf 1 gene and molecular marker-assisted development of elite radish (Raphanus sativus L.) CMS lines with a functional marker for fertility restoration

et al. 2012

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Cytoplasmic male sterility (CMS) is a maternally inherited trait that is widely utilized in hybrid seed production. In this study, the Rs-Rf 1 gene and its allele, the Rs-rf 1 gene, were isolated from a radish CMS restorer line and maintainer, respectively. The Rs-Rf 1 gene was identified as a member of the pentatricopeptide repeat family. The results of reverse transcription (RT)-PCR and quantitative RT-PCR analyses of the floral buds at different development stages were used to characterize the expression features of Rs-Rf 1 and orf138, a unique transcribed gene in the mitochondrion for its role in determining male sterility in the cytoplasm. orf138 was upregulated in the CMS line and F 1 hybrid at three bud developmental stages, including meiosis and the tetroid and microspore stages, but it was not expressed in the maintainer and restorer line, suggesting that the Rs-Rf 1 gene was not able to block orf138 transcription. As in the F 1 plant, the transcription of Rs-Rf 1 was gradually up-regulated in the restorer line with progressive stages of bud development, but it was not transcribed in the CMS line and maintainer. Different restriction sites for SspI between the allele of Rs-Rf 1 and Rs-rf 1 were also identified, and a guideline for using the functional marker to distinguish the genotypes of individuals with Rs-Rf 1 was developed. Due to its co-dominant character, this marker could be used to discriminate the heterozygous and homozygous fertility-restoring allele. Using this functional marker, we established a system of molecular marker-assisted development of elite CMS lines in radish. This system will contribute greatly to candidate maintainer identification and accelerate the process of elite CMS line development in late-bolting radish breeding programs.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of the Rs-Rf 1 gene and molecular marker-assisted development of elite radish (Raphanus sativus L.) CMS lines with a functional marker for fertility restoration

et al. 2012

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“…Raphanus sativus L. (Brassicaceae) provides an ideal opportunity for overcoming this difficulty. In this species, the sex phenotype of individuals is determined by the presence or absence of only one CMS gene and by one (or more) dominant Rf gene(s) that interacts with the CMS gene (Yamagishi & Terachi, 1994; Yasumoto et al. , 2008).…”

Section: Introductionmentioning

confidence: 99%

Relative fitness of females and hermaphrodites in a natural gynodioecious population of wild radish,Raphanus sativusL. (Brassicaceae): comparison based on molecular genotyping

Miyake

Terachi

et al. 2009

J of Evolutionary Biology

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In many gynodioecious species, sex determination involves both cytoplasmic male‐sterility (CMS) genes and nuclear genes that restore male function. Differences in fitness among genotypes affect the dynamics of those genes, and thus that of gynodioecy. We used a molecular marker to discriminate between hermaphrodites with and without a CMS gene in gynodioecious Raphanus sativus. We compared fitness through female function among the three genotypes: females, hermaphrodites with the CMS gene and those without it. Although there was no significant difference among the genotypes in seed size, hermaphrodites without the CMS gene produced significantly more seeds, and seeds with a higher germination rate than the other genotypes, suggesting no fitness advantage for females and no benefit to bearing the CMS gene. Despite the lack of fitness advantage for females in the parameter values we estimated, a theoretical model of gynodioecy shows it can be maintained if restorer genes impose a cost paid in pollen production. In addition, we found that females invest more resources into female reproduction than hermaphrodites when they become larger. If environmental conditions enable females to grow larger this would facilitate the dynamics of CMS genes.

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“…However, the exact length of exogenous radish chromosome fragments is unclear in all three lines. Other researchers reported that the restorer gene from different sources, including R. raphanistrum from Turkey, the Japanese wild radish, European cultivars, and lines from China, resulted in the same or similar loci [ 48 , 49 ]. However, the length of the exogenous radish chromosome was expected to be shorter than that of R2000, because R2000 was bred using irradiation to reduce the length of exogenous radish chromosome fragments [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

Cytological and Molecular Characterization of a New Ogura Cytoplasmic Male Sterility Restorer of Brassica napus L.

Huang,

Ren,

Lin

et al. 2024

Plants

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Ogura cytoplasmic male sterility (CMS) is considered the rapeseed (Brassica napus L.) with the most potential to be utilized as a heterosis system worldwide, but it lacks sufficient restorers. In this study, root tip cell (RTC) mitotic and pollen mother cell (PMC) meiosis observations were compared to ensure the number of chromosomes and the formation of a chromosomal bridge using restorer lines R2000, CLR650, and Zhehuhong (a new restorer) as the experimental material. Further, molecular markers of exogenous chromosomal fragments were detected and the sequence and expression differences of restorer genes in the three lines were determined to identify the distinctive characteristics of Zhehuhong. The results showed that the number of chromosomes in Zhehuhong was stable (2n = 38), indicating that the exogenous radish chromosome segment had been integrated into the chromosome of Zhehuhong. Molecular marker detection revealed that Zhehuhong was detected at most loci, with only the RMA05 locus being missed. The exogenous radish chromosome segment of Zhehuhong differed from R2000 and CLR650. The pollen mother cells of Zhehuhong showed chromosome lagging in the meiotic metaphase I, meiotic anaphase I, and meiotic anaphase II, which was consistent with R2000 and CLR650. The restorer gene PPRB in Zhehuhong had 85 SNPs compared with R2000 and 119 SNPs compared with CLR650, indicating the distinctive characteristic of PPRB in Zhehuhong. In terms of the spatial expression of PPRB, the highest level was detected in the anthers in the three restorer lines. In addition, in terms of temporal expression, the PPRB gene expression of Zhehuhong was highest at a bud length of 4 mm. Our results clearly indicated that Zhehuhong is a new restorer line for the Ogura CMS system, which can be used further in rapeseed heterosis utilization.

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Genetic and molecular analysis of the restoration of fertility (Rf) genes for Ogura male-sterility from a Japanese wild radish (Raphanus sativus var. hortensis f. raphanistroides Makino)

Abstract: Genetic and molecular analysis of the restoration of fertility (Rf) genes for Ogura male-sterility from a Japanese wild radish (Raphanus sativus var. hortensis f. raphanistroides Makino)Abstract Ogura male-sterile cytoplasm is one of the most extensively studied cytoplasms in Brassicaceae.

Cited by 7 publications

References 11 publications

Molecular characterization of the Rs-Rf 1 gene and molecular marker-assisted development of elite radish (Raphanus sativus L.) CMS lines with a functional marker for fertility restoration

Molecular characterization of the Rs-Rf 1 gene and molecular marker-assisted development of elite radish (Raphanus sativus L.) CMS lines with a functional marker for fertility restoration

Relative fitness of females and hermaphrodites in a natural gynodioecious population of wild radish,Raphanus sativusL. (Brassicaceae): comparison based on molecular genotyping

Cytological and Molecular Characterization of a New Ogura Cytoplasmic Male Sterility Restorer of Brassica napus L.

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