Genetic and gut microbiome determinants of SCFA circulating and fecal levels, postprandial responses and links to chronic and acute inflammation

Nogal, Ana; Asnicar, Francesco; Vijay, Amrita; Kouraki, Afroditi; Visconti, Alessia; Louca, Panayiotis; Wong, Kari E.; Baleanu, Andrei-Florin; Giordano, Francesca; Wolf, Jonathan; Hadjigeorgiou, George; Davies, Richard; Michelotti, Gregory A.; Franks, Paul W.; Berry, Sarah; Falchi, Mario; Ikram, Adeel; Ollivere, B.; Zheng, Amy; Nightingale, Jessica; Mangino, Massimo; Segata, Nicola; Bulsiewicz, William J.; Spector, Tim D.; Valdes, Ana M.; Menni, Cristina

doi:10.1080/19490976.2023.2240050

Cited by 6 publications

(3 citation statements)

References 45 publications

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“…Moreover, the abundance of the Firmicutes, Bacteroidetes, and Proteobacteria phyla accounted for a large proportion of the gut microbiota among all intestinal segments in pigs in our study, which was in line with previous studies [21]. SCFAs, such as butyrate, play a pivotal role in connecting microbiota functions to diverse biological effects [22][23][24][25]. Specifically, butyrate represents a significant energy source for the colonic epithelium [26,27].…”

Section: Discussionsupporting

confidence: 91%

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Clostridium butyricum and carbohydrate active enzymes contribute to the reduced fat deposition in pigs

Ma,

Tao,

Song

et al. 2024

iMeta

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Pig gastrointestinal tracts harbor a heterogeneous and dynamic ecosystem populated with trillions of microbes, enhancing the ability of the host to harvest energy from dietary carbohydrates and contributing to host adipogenesis and fatness. However, the microbial community structure and related mechanisms responsible for the differences between the fatty phenotypes and the lean phenotypes of the pigs remained to be comprehensively elucidated. Herein, we first found significant differences in microbial composition and potential functional capacity among different gut locations in Jinhua pigs with distinct fatness phenotypes. Second, we identified that Jinhua pigs with lower fatness exhibited higher levels of short‐chain fatty acids in the colon, highlighting their enhanced carbohydrate fermentation capacity. Third, we explored the differences in expressed carbohydrate‐active enzyme (CAZyme) in pigs, indicating their involvement in modulating fat storage. Notably, Clostridium butyricum might be a representative bacterial species from Jinhua pigs with lower fatness, and a significantly higher percentage of its genome was dedicated to CAZyme glycoside hydrolase family 13 (GH13). Finally, a subsequent mouse intervention study substantiated the beneficial effects of C. butyricum isolated from experimental pigs, suggesting that it may possess characteristics that promote the utilization of carbohydrates and hinder fat accumulation. Remarkably, when Jinhua pigs were administered C. butyricum, similar alterations in the gut microbiome and host fatness traits were observed, further supporting the potential role of C. butyricum in modulating fatness. Taken together, our findings reveal previously overlooked links between C. butyricum and CAZyme function, providing insight into the basic mechanisms that connect gut microbiome functions to host fatness.

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Section: Discussionsupporting

confidence: 91%

“…SCFAs, such as butyrate, play a pivotal role in connecting microbiota functions to diverse biological effects [ 22 , 23 , 24 , 25 ]. Specifically, butyrate represents a significant energy source for the colonic epithelium [ 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Clostridium butyricum and carbohydrate active enzymes contribute to the reduced fat deposition in pigs

Ma,

Tao,

Song

et al. 2024

iMeta

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“…Some studies indicate that breast milk SCFAs may be produced by the maternal gut microbiota and distributed to the breast through circulation; however, it is also suggested that they could be produced in breast milk by its own microbiota (Stinson et al, 2020 ; Stinson and Geddes, 2022 ). Currently, increasing evidence demonstrates that SCFAs play key roles in infant growth and development (Nogal et al, 2023 ). In vitro and in vivo studies have found that the breast milk SCFAs could help to decrease inflammation, increase immunity, and reduce intestinal inflammation (Gao et al, 2021 ; Xu et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Short-chain fatty acids in breast milk and their relationship with the infant gut microbiota

Xi,

Yan,

Duan

et al. 2024

Front. Microbiol.

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IntroductionThe short-chain fatty acids (SCFAs) contained in breast milk play a key role in infant growth, affecting metabolism and enhancing intestinal immunity by regulating inflammation.MethodsIn order to examine the associations between the microbiota and SCFA levels in breast milk, and explore the roles of SCFAs in regulating the infant gut microbiota, we enrolled 50 paired mothers and infants and collected both breast milk and infant fecal samples. Breast milk SCFA contents were determined by UPLC-MS, and whole genome shotgun sequencing was applied to determine the microbial composition of breast milk and infant feces. The SCFA levels in breast milk were grouped into tertiles as high, medium, or low, and the differences of intestinal microbiota and KEGG pathways were compared among groups.ResultsThe results demonstrated that breast milk butyric acid (C4) is significantly associated with Clostridium leptum richness in breastmilk. Additionally, the specific Bifidobacterium may have an interactive symbiosis with the main species of C4-producing bacteria in human milk. Women with a low breast milk C4 tertile are associated with a high abundance of Salmonella and Salmonella enterica in their infants' feces. KEGG pathway analysis further showed that the content of C4 in breast milk is significantly correlated with the infants' metabolic pathways of lysine and arginine biosynthesis.DiscussionThis study suggests that interactive symbiosis of the microbiota exists in breast milk. Certain breast milk microbes could be beneficial by producing C4 and further influence the abundance of certain gut microbes in infants, playing an important role in early immune and metabolic development.

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Short-chain fatty acids (SCFA) in infants’ plasma and corresponding mother’s milk and plasma in relation to subsequent sensitisation and atopic disease

Barman,

Gio-Batta,

Andrieux

et al. 2024

eBioMedicine

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Genetic and gut microbiome determinants of SCFA circulating and fecal levels, postprandial responses and links to chronic and acute inflammation

Cited by 6 publications

References 45 publications

Clostridium butyricum and carbohydrate active enzymes contribute to the reduced fat deposition in pigs

Clostridium butyricum and carbohydrate active enzymes contribute to the reduced fat deposition in pigs

Short-chain fatty acids in breast milk and their relationship with the infant gut microbiota

Short-chain fatty acids (SCFA) in infants’ plasma and corresponding mother’s milk and plasma in relation to subsequent sensitisation and atopic disease

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