Genetic and Genomic Tools for<i>Cannabis sativa</i>

Vergara, Daniela; Baker, Halie; Clancy, Kayla; Keepers, Kyle G.; Mendieta, J. Paul; Pauli, Christopher S.; Tittes, Silas; White, Kristin; Kane, Nolan C.

doi:10.1080/07352689.2016.1267496

Cited by 54 publications

(59 citation statements)

References 86 publications

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“…Correlating this increase in scientific publications with methodological advances in DNA research [19,20,22], it seems that only the advent of next-generation sequencing (NGS) in 2005 boosted genetics studies on Cannabis, mainly in the last decade. Recently, NGS approaches have been employed for the complete sequencing of the Cannabis chloroplast [5,23] and mitochondria genomes [24], to generate whole-genome drafts [25], and for the development of SNP markers for different varieties of Cannabis [4].…”

Section: Discussionmentioning

confidence: 99%

A Bibliometric Analysis of Cannabis Publications: Six Decades of Research and a Gap on Studies with the Plant

et al. 2018

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In this study we performed a bibliometric analysis focusing on the general patterns of scientific publications about Cannabis, revealing their trends and limitations. Publications related to Cannabis, released from 1960 to 2017, were retrieved from the Scopus database using six search terms. The search term “Genetics” returned 53.4% of publications, while “forensic genetics” and “traceability” represented 2.3% and 0.1% of the publications, respectively. However, 43.1% of the studies were not directly related to Cannabis and, in some cases, Cannabis was just used as an example in the text. A significant increase in publications was observed after 2001, with most of the publications coming from Europe, followed by North America. Although the term Cannabis was found in the title, abstract, or keywords of 1284 publications, we detected a historical gap in studies on Cannabis. We expect that increasing interest in this issue and the rise of new biotechnological advances will lead to the development of new studies. This study will help scientists identify overall research needs, detect the scientific areas in evidence concerning Cannabis studies, and find excellent centers of investigation for scientific interchange and collaboration.

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Section: Discussionmentioning

confidence: 99%

A Bibliometric Analysis of Cannabis Publications: Six Decades of Research and a Gap on Studies with the Plant

et al. 2018

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“…However, our tree models for the overall SNP frequencies of the population groups inferred by FLOCK (Figure 3) demonstrate that each group still contains strong genetic signals from ancestral biogeographic gene pools. Still, our results and the incongruence between Cannabis genomic analyses (Sawler et al, 2015) reinforces the importance of using many high-quality single-copy regions of the genome, rather than smaller numbers of loci that could lead to lower resolution or even misleading results (Vergara et al, 2017).…”

Section: Tests Of Tree Modelsmentioning

confidence: 95%

“…Our findings are contrary to those of Sawler et al, (2015), which resulted in hemp samples having significantly more heterozygosity than drug-type varieties. In both analyses, hemp samples numbers were limited, and the two different sequencing library preparation methods make reconciling these conflicting results impossible without further sampling (Vergara et al, 2017).…”

Section: B Phylogenetic Relationshipsmentioning

confidence: 99%

Genomic and Chemical Diversity inCannabis

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Vergara

Tittes

et al. 2016

Critical Reviews in Plant Sciences

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Plants of the Cannabis genus are the only prolific producers of phytocannabinoids, compounds that strongly interact with the evolutionarily ancient endocannabinoid receptors shared by most bilaterian taxa. For millennia, the plant has been cultivated not only for these compounds, but also for food, rope, paper, and clothing. Today, specialized varieties yielding high-quality textile fibers, nutritional seed oil, or high cannabinoid content are cultivated across the globe. However, the genetic identities and histories of these diverse populations remain largely obscured. We analyzed the nuclear genomic diversity among 340 Cannabis varieties, including fiber and seed oil hemp, high cannabinoid drug-types, and feral populations. These analyses demonstrate the existence of at least three major groups of diversity with European hemp varieties more closely related to narrow leaflet drug-types (NLDTs) than to broad leaflet drug-types (BLDTs). The BLDT group appears to encompass less diversity than the NLDT, which reflects the larger geographic range of NLDTs, and suggests a more recent origin of domestication of the BLDTs. As well as being genetically distinct, hemp, NLDT, and BLDT genetic groups produce unique cannabinoid and terpenoid content profiles. This combined analysis of population genomic and trait variation informs our understanding of the potential uses of different genetic variants for medicine and agriculture, providing valuable insights and tools for a rapidly emerging valuable industry.

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“…The United States is currently experiencing drastic changes in patterns of Cannabis use associated with widespread relaxation of laws that previously limited both medical and recreational marijuana consumption 3 and hemp cultivation. This has led to a need for extensive research into the basic biology and taxonomy of Cannabis sativa 4–8 , and the possible benefits and threats from Cannabis consumption 3,9 .…”

Section: Introductionmentioning

confidence: 99%

Research grade marijuana supplied by the National Institute on Drug Abuse is genetically divergent from commercially availableCannabis

et al. 2019

Preprint

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Public comfort withCannabis(marijuana and hemp) has recently increased, resulting in previously strictCannabisregulations now allowing hemp cultivation, medical use, and in some states, recreational consumption. There is a growing interest in the potential medical benefits of the various chemical constituents produced by theCannabisplant. Currently, the University of Mississippi, funded through the National Institutes of Health/National Institute on Drug Abuse (NIH/NIDA), is the sole Drug Enforcement Agency (DEA) licensed facility to cultivateCannabisfor research purposes. Hence, most federally funded research where participants consumeCannabisfor medicinal purposes relies on NIDA-supplied product. Previous research found that cannabinoid levels in research grade marijuana supplied by NIDA did not align with commercially availableCannabisfrom Colorado, Washington and California. Given NIDA chemotypes were misaligned with commercialCannabis, we sought to investigate where NIDA’s research grade marijuana falls on the genetic spectrum ofCannabisgroups. NIDA research grade marijuana was found to genetically group with Hemp samples along with a small subset of commercial drug-typeCannabis. A majority of commercially available drug-typeCannabiswas genetically very distinct from NIDA samples. These results suggest that subjects consuming NIDA research grade marijuana may experience different effects than average consumers.

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Genetic and Genomic Tools forCannabis sativa

Cited by 54 publications

References 86 publications

A Bibliometric Analysis of Cannabis Publications: Six Decades of Research and a Gap on Studies with the Plant

A Bibliometric Analysis of Cannabis Publications: Six Decades of Research and a Gap on Studies with the Plant

Genomic and Chemical Diversity inCannabis

Research grade marijuana supplied by the National Institute on Drug Abuse is genetically divergent from commercially availableCannabis

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