Genetic and functional heterogeneities among fluorescent Pseudomonas isolated from environmental samples

Mehri, Inès; Turki, Yousra; Chérif, Hanène; Hassen, A.; Meyer, Jean‐Marie; Gtari, Maher

doi:10.2323/jgam.57.101

Cited by 23 publications

(25 citation statements)

References 41 publications

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“…16S rDNA genes from nine strains used to compare DGGE profiles are also amplified with the same primers (PsF, PsR, and F968‐GC, Psr). * PsWw.128: P. fluorescens (I), PsS.83: P. putida (I), PsS.73: P. fluorescens (II), PsS.79: P. putida (II), PsWt.146: P. putida (II), PsTp.171: P. mosselii , PsWw.118: P. otitidis , PsC.132: P. aeruginosa and Syr: P. syringae . Access numbers of selected strains are respectively: HM627582‐HM627581‐HM627594‐HM627629‐HM627604‐HM627603‐HM627606‐HM627574‐AM265392.…”

Section: Methodsmentioning

confidence: 99%

Influence of Biological Treatment and Ultraviolet Disinfection System on Pseudomonas spp. Diversity in Wastewater as Assessed by Denaturing Gradient Gel Electrophoresis

Mehri

Turki

Chérif

et al. 2013

CLEAN Soil Air Water

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Biological treatment methods use natural processes of ubiquitous living organisms to improve or upgrade the quality of a wastewater. To investigate the dominance of Pseudomonas spp. community in untreated and treated wastewater from full-scale biodiscs treatment plant, PCR-denaturing gradient gel electrophoresis coupled with sequence analysis of 16S rRNA gene fragments from dominant bands, were performed. The Pseudomonas species distribution over the total treatment process and over seasons (summer and winter) was obtained by comparing the denaturing gradient gel electrophoresis (DGGE) patterns to a normalized mix of nine reference Pseudomonas strains. Results indicated that Pseudomonas putida and Pseudomonas fluorescens strains showed dominance in wastewater, remarkable stability in the biofilm and resistance to UV irradiation. A reduction of Pseudomonas aeruginosa in treated wastewater was also observed. In addition, climatic and operational conditions results in selection of microbial community. The high temperature and intense solar radiation contribute to the total absence of the Pseudomonas syringae strain. In this work, the effect of increasing UV 254 germicidal dose on the Pseudomonas community in secondary treated wastewater effluent was also tested. At a dose of 1200 mWs/cm 2 , total disappearance of the band corresponding to P. aeruginosa, was noted. The DGGE approach can be used as an effective method to assess directly the Pseudomonas community shifts in studied wastewater treatment plant.

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Section: Methodsmentioning

confidence: 99%

Influence of Biological Treatment and Ultraviolet Disinfection System on Pseudomonas spp. Diversity in Wastewater as Assessed by Denaturing Gradient Gel Electrophoresis

Mehri

Turki

Chérif

et al. 2013

CLEAN Soil Air Water

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“…While P. aeruginosa is an important opportunist pathogen responsible for worldwide nosocomial infections, other species such as P. syringae is wide known as being deleterious to plants, and P. putida or P. fluorescens are considered plant growth promoters (Mehri et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Genetic Lineages and Antimicrobial Resistance inPseudomonasspp. Isolates Recovered from Food Samples

Estepa

Rojo–Bezares

Torres

et al. 2015

Foodborne Pathogens and Disease

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Raw food is a reservoir of Pseudomonas isolates that could be disseminated to consumers. The presence of Pseudomonas spp. was studied in food samples, and the phenotypic and genotypic characterizations of the recovered isolates were analyzed. Two samples of meat (3%, turkey and beef) and 13 of vegetables (22%, 7 green peppers and 6 tomatoes) contained Pseudomonas spp. A total of 20 isolates were identified, and were classified as follows (number of isolates): P. aeruginosa (5), P. putida (5), P. nitroreducens (4), P. fulva (2), P. mosselli (1), P. mendocina (1), P. monteilii (1), and Pseudomonas sp. (1). These 20 Pseudomonas isolates were clonally different by pulsed-field-gel-electrophoresis, and were resistant to the following antibiotics: ticarcillin (85%), aztreonam (30%), cefepime (10%), imipenem (10%), and meropenem (5%), but were susceptible to ceftazidime, piperacillin, piperacillin-tazobactam, doripenem, gentamicin, tobramycin, amikacin, ciprofloxacin, norfloxacin, and colistin. Only one strain (Ps158) presented a class 1 integron lacking the 3' conserved segment. The five P. aeruginosa strains were typed by multilocus sequence typing in five different sequence-types (ST17, ST270, ST800, ST1455, and ST1456), and different mutations were detected in protein OprD that were classified in three groups. One strain (Ps159) showed a new insertion sequence (ISPa47) truncating the oprD gene, and conferring resistance to imipenem.

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“…tarda isolates. Enterobacterial repetitive intergenic consensus (ERIC) PCR, the repetitive extragenic palindromic (REP) PCR and BOX‐based REP‐PCR (BOX‐PCR) are the three types of interspersed repetitive elements sequence‐based PCR fingerprinting (rep‐PCR), which enable differentiation of eubacterial species and strains (Gillings and Holley 1997; Rice 2009; Mehri et al. 2011).…”

Section: Introductionmentioning

confidence: 99%

Genetic relationships of Edwardsiella strains isolated in China aquaculture revealed by rep-PCR genomic fingerprinting and investigation of Edwardsiella virulence genes

Wang

Xiao

et al. 2011

Journal of Applied Microbiology

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Aims: The aim of this study was to classify Edwardsiella strains isolated from China aquaculture based on biochemical and molecular methods. Methods and Results: In this study, biochemical characterization of 19 Edwardsiella tarda isolates and two Edwardsiella ictaluri isolates was performed with API 20E system. Other pathogenicity‐related phenotypes such as haemagglutination, haemolytic activities and lethality to fish were also examined in these strains. As it was difficult to categorize the subgroups of Edw. tarda according to their origins or phenotypic properties, three PCR‐based methods, i.e. PCR amplification of virulence genes, Enterobacterial repetitive intergenic consensus‐PCR and BOX‐PCR, were carried out to further resolve the relatedness of the Edw. tarda isolates. As a result, all Edw. tarda isolates could be generally grouped into pathogenic and nonpathogenic branches before being classified into strain‐specific or origin‐specific clades. Conclusions: Biochemical characterization was sensitive for interspecific typing, while PCR‐based approaches permitted a more accurate discrimination for intraspecific typing resulting in pathogenic and nonpathogenic clusters and further more delicate clades for Edwardsiella. Significance and Impact of the Study: PCR‐based genomic fingerprinting to study the relatedness and trace the pathogenicity of the Edwardsiella strains will be helpful in investigating the virulence factors of Edwardsiella and in the development of vaccines and diagnostics for edwardsiellosis.

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Genetic and functional heterogeneities among fluorescent Pseudomonas isolated from environmental samples

Cited by 23 publications

References 41 publications

Influence of Biological Treatment and Ultraviolet Disinfection System on Pseudomonas spp. Diversity in Wastewater as Assessed by Denaturing Gradient Gel Electrophoresis

Influence of Biological Treatment and Ultraviolet Disinfection System on Pseudomonas spp. Diversity in Wastewater as Assessed by Denaturing Gradient Gel Electrophoresis

Genetic Lineages and Antimicrobial Resistance inPseudomonasspp. Isolates Recovered from Food Samples

Genetic relationships of Edwardsiella strains isolated in China aquaculture revealed by rep-PCR genomic fingerprinting and investigation of Edwardsiella virulence genes

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