Genetic and Enzymatic Properties of Metallo-β
            <b>-</b>
            Lactamase VIM-5 from a Clinical Isolate of
            <i>Enterobacter cloacae</i>

Gacar, Gülçin; Midilli, Kenan; Kolayli, Fetiye; Ergen, Kıvanç; Gündeş, Sibel; Hoşoğlu, Salih; Karadenizli, Aynur; Vahaboğlu, Haluk

doi:10.1128/aac.49.10.4400-4403.2005

Cited by 33 publications

(30 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In 2009 a study among ESBL isolates showed that 2.5% of E. coli and 7.84% of K. pneumoniae were carbapenem resistant [2,3]. The carbapenemases described in Turkey were the OXA-48 and VIM-5 [1,[6][7]. One case of a K. pneumoniae producing OXA-48 was reported in an Egyptian patient hospitalized in France [11].…”

Section: Discussionmentioning

confidence: 99%

“…The introduction of such resistant strains, particularly those carrying the NDM-1 gene, constitutes an alarming threat. Reports on carbapenem resistant E. coli and K. pneumoniae have been published from different countries in the region including Turkey [1,[5][6][7], Greece [8][9][10], Egypt [11], Saudi Arabia [12], Oman [13], Israel [14], and Iraq [15].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae producing NDM-1 in Lebanon

El-Herte

Araj

Matar

et al. 2012

J Infect Dev Ctries

View full text Add to dashboard Cite

Carbapenem resistance has been encountered globally with poor outcome of infected patients. NDM-1 (New Delhi metallo-beta-lactamase) gene containing organisms have emerged and are now spreading in all continents. This is the first report of Iraqi patients referred to Lebanon from whom carbapenem resistant Enterobacteriaceae were recovered. The genes involved in carbapenem resistance were bla-OXA-48 and the novel NDM-1. This report highlights the alarming introduction of such resistance among Enterobacteriaecae to this country.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Detection of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae producing NDM-1 in Lebanon

El-Herte

Araj

Matar

et al. 2012

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…Class 1 integron positive gram-negative bacteria includes Acinetobacter, Aeromonas, Alacaligenes, Burkholderia, Campylobacter, Citrobacter, Enterobacter, Escherichia, Unsellable, Pseudomonas, Salmonella, Serratia, Shigella, Stenotrophomonas and Vibrio [21]. Also, the presence of class 1 integrons has been shown in various gram-negative bacteria in our country, Turkey [22][23][24].…”

Section: Discussionmentioning

confidence: 99%

A Study on Class I Integrons and Antimicrobial Resistance among Clinical Staphylococci Isolates from a Turkish Hospital

Güney¹

2014

Clin Microbiol

View full text Add to dashboard Cite

Purpose: Methicillin-resistant staphylococci (MRS) are increasingly significant in the hospital setting due to multidrug resistance. Integrons are genetic elements that can play a role in the dissemination of antimicrobial resistance. While their role is well established in gram-negative bacteria, less is known about the presence of integrons in gram-positive bacteria. Our aim was to investigate the presence of class 1 integrons in clinical MRS isolates. Methods:One hundred clinical MRS isolates were included in the study. Identification of MRS isolates was confirmed by multiplex PCR in which staphylococcal 16S rRNA, nuc and mecA genes-specific primers were used. The presence of class 1 integron was investigated by PCR with intI 1-specific primers.Results: All methicillin-resistant Staphylococcus aureus (MRSA) isolates were positive for 16S rRNA, nuc and mecA genes. All methicillin-resistant coagulase negative staphylococci (MRCoNS) were positive for 16S rRNA and mecA, and negative for nuc gene. The existence of class 1 integron was not detected in all the isolates tested. Conclusions: Class 1 integron positive clinicial MRS strains have only been shown in a few studies from East Asia. Further studies are needed in order to determine the significance of integrons in staphylococci and their role in DNA transfer between gram-positive and gram-negative bacteria.

show abstract

“…9,10 Di yar ba kır' dan 2002 yı-lın dan ön ce izo le edi len bir En te ro bac ter clo a ca e kö ke nin den VIM-5 ti pi MBL izo le edil miş tir. 11 Son za man lar da bir çok önem li Gram ne ga tif bak te ri de MBL sap tan ma sık lık la rı ve tip le ri hız la art mak ta dır. Ör ne ğin; baş lan gıç ta sa de ce Ja pon ya' -ya ve ba zı Uzak Do ğu As ya ül ke le riy le sı nır lı ol duğu dü şü nü len IMP ti pi MBL bir çok fark lı ül ke den bil di ril miş ve IMP ti pi MBL'ın sa yı sı gü nü müz de 20'yi aş mış tır.…”

Section: Clinical Microbiologyunclassified

A New Multiplex PCR Method for Rapid Detection of Genes Encoding VIM and IMP Types of Metallo Beta Lactamases

Küçükbasmacı¹,

Midilli²,

Issa³

et al. 2010

Turkiye Klinikleri J Med Sci

View full text Add to dashboard Cite

Ö ÖZ ZE ET T A Am ma aç ç: : Bu ça lış ma da VIM ve IMP ti pi me tal lo be ta-lak ta maz la rı (MBL) kod la yan gen le rin tama mı nı hız lı şekil de sap ta ya cak mul tip leks po li me raz zin cir re ak si yo nu (PCR) pro to ko lü ta rif edilmek te dir. Ay rı ca bu yön tem İstan bul' da bü yük bir üni ver si te has ta ne sin de izo le edi len P.ae ru gi no sa kö ken le rin de MBL var lı ğı ve tip le ri nin araş tı rıl ma sın da kul la nıl mış tır. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : bla IMP1-22 ve bla VIM1-12 pro to tip se kans la rı Gen Bank 'tan in di ril miş ve Bi o e dit bil gi sa yar pa ke tinden Clus talW mul tipl align ment prog ra mı kul la nı la rak hi za lan mış tır. Fark lı pri mer kon san tras yon ve oran la rı nın kul la nıl dı ğı op ti mi zas yon de ney le rin den son ra en uy gun mul tip leks PCR for ma tı 1:2 VIM / IMP ola rak be lir len miş ve tüm ta ra ma test le rin de bu for mat kul la nıl mış tır. Mul tip leks PCR yön te mi imi pe ne me di renç li ve ya or ta di renç li 51 adet P.ae ru gi no sa kö ke nin de de kul la nıl mış -tır. İmi pe nem, me ro pe nem, sef ta zi dim ve se fe pim için mi ni mum in hi bi tör kon san tras yon la rı (MİK) agar di lüs yon yön te mi ile sap tan mış tır. Kon trol kö ken le ri ola rak IMP-1, IMP2, IMP13, VIM1, VIM2, VIM4 ti pi en zim le ri üre ten P.ae ru gi no sa kö ken le ri ve VIM-5 ti pi en zim üre ten K.pne u moni a e kö ke ni kul la nıl mış tır. B Bu ul l g gu u l la ar r: : Tüm kon trol kö ken le ri be lir ti len mul tip leks PCR yön te mi ile tes pit edil miş tir. Elli bir adet P.eru gi no sa kö ke nin de mul tip leks PCR yön te mi ile bla IMP ve bla -VIM var lı ğı sap tan ma mış tır. İmi pe nem, me ro pe nem, sef ta zi dim ve se fe pim için MİK50 de ğer le ri sı -ra sıy la 32μg/ml, 32μg/ml, 128μg/ml, 64μg/ml ola rak bu lun muş tur. S So o n nu uç ç: : Bu ça lış ma da be lir ti len mul tip leks PCR pro to ko lü IMP ve VIM ti pi MBL gen le ri nin çe şit li kli nik bi rim ler de epi de mi yo lojik ya yı lım la rı nı in ce le mek ama cıy la kul la nıl ma sı fay da lı ola bi le cek bir yön tem dir.A An na ah h t ta ar r K Ke e l li i m me e l le er r: : Pseudomonas aeruginosa; PCR; karbapenemler; beta-laktamazlar A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : This study des cri bes a mul tip lex polymerase chain reaction (PCR) as say proto col for ra pid de tec ti on of ge nes en co ding VIM and IMP types of me tal lo be ta lac ta ma ses (MBL). This as say is al so used for eva lu a te the pre sen ce and types of MBL among Pse u do mo nas ae ru gi nosa iso la tes in a lar ge uni ver sity hos pi tal in Is tan bul. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : bla IMP1-22 and bla -VIM1-12 pro toty pe se qu en ces we re down lo a ded from Gen Bank and alig ned using Clus talW mul tiple align ment prog ram in Bi o e dit soft wa re pac ka ge. Fol lo wing op ti mi sa ti on using dif fe rent con cen tra ti ons and com bi na ti on of pri mers, the 1.2 VIM/IMP ra ti o for pri mers we re de ter mi ned and used thro ...

show abstract

Genetic and Enzymatic Properties of Metallo-β - Lactamase VIM-5 from a Clinical Isolate of Enterobacter cloacae

Cited by 33 publications

References 18 publications

Detection of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae producing NDM-1 in Lebanon

Detection of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae producing NDM-1 in Lebanon

A Study on Class I Integrons and Antimicrobial Resistance among Clinical Staphylococci Isolates from a Turkish Hospital

A New Multiplex PCR Method for Rapid Detection of Genes Encoding VIM and IMP Types of Metallo Beta Lactamases

Contact Info

Product

Resources

About