Genetic and DNA-methylation changes induced by potassium dichromate in Brassica napus L.

Labra, Massimo; Grassi, F.; Imazio, Serena; Fabio, Tiziana Di; Citterio, Sandra; Sgorbati, Sergio; Agradi, E.

doi:10.1016/j.chemosphere.2003.10.024

Cited by 109 publications

(64 citation statements)

References 39 publications

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“…The exposure of plants to diverse, abnormal temperatures or photoperiods can be a strong stress factor. Changes in the level of DNA methylation in MSAP analyses were reported in response to various environmental factors or treatments (Tatra et al 2000;Labra et al 2004;Baranek et al 2010). In rapeseed, the methylation/demethylation events at MSAP loci were the result of the salt stress (Lu et al 2007) and in Zea mays roots a genome-wide demethylation was observed under cold stress (Steward et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Vernalization and photoperiod-related changes in the DNA methylation state in winter and spring rapeseed

et al. 2012

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Vernalization-induced flowering is an effect of the epigenetic regulation of gene expression through DNA methylation and histone modifications. Vernalizationmediated silencing of a floral repressor through histone modifications was shown in Arabidopsis thaliana. However, for Brassica napus L., the mechanism underlying vernalization is unclear, and the roles of DNA methylation and histone modifications have not been established. This study revealed the profiles of changes in the DNA methylation state during vernalization (after 14, 35, 56 days) and the subsequent growth in long-or short-day photoperiods (after 2, 7, 14 days) in the winter and spring rapeseed using TLC and MSAP techniques. TLC analysis showed a significant decrease in the amount of 5-methylcytosine (m 5 C) in genomic DNA in both cultivars at the beginning of vernalization, but upon its termination, the winter rape showed a reduced level of m 5 C contrary to a significantly increased level in the spring rape. MSAP analysis revealed that winter and spring rapeseed differed in the MSAP loci which were demethylated/methylated in the course of the experiment and presented diverse profiles of changes in the methylation state. The winter rape showed permanent demethylations at 69.2 % of MSAP loci in the course of vernalization that were mostly preserved upon its termination. The spring rape showed similar numbers of demethylations and methylations that were mainly transient. The study provides evidence of the role of DNA methylation in vernalization for rapeseed and for the significant prevalence of demethylations at the beginning of vernalization, which is necessary for the transition to reproductive growth.

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Section: Discussionmentioning

confidence: 99%

Vernalization and photoperiod-related changes in the DNA methylation state in winter and spring rapeseed

et al. 2012

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“…The methylation level of rape genomic DNA has been suggested to be altered by chromium. Using MSAP and immuno-labeling techniques, the analysis revealed that the level of hypermethylation in the rape genome was correlated positively with the stress dosage of chromium, which suggested de novo synthesis of methylated cytosine (Labra et al 2004 ). However, in some reports, a contrary trend was observed.…”

Section: Metal Ions and Plant Genomic Dna Methylationmentioning

confidence: 98%

Epigenetic Mechanisms in Plants: An Overview

Munshi

Ahuja²,

Bahadur

2015

Plant Biology and Biotechnology

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Plant epigenetics has become one of the hottest topics of research not only as a subject of basic research but also as a possible new source of benefi cial traits for plant breeding. In addition, epigenetic mechanisms are also crucial to appropriate plant reactions to stress. Given the sessile lifestyle and the late differentiation of the germ line, plants can perceive stresses during vegetative growth and also memorize them, possibly by epigenetic mechanisms. Plants use three systems to initiate and regulate epigenetic gene regulation, like other higher organisms, which include DNA methylation, histone modifi cations, and RNA interference. New concepts are being evolved to show how these epigenetic components interact and stabilize each other. The role of epigenetic mechanisms in hybrid vigor and epigenetic transgene silencing is also being explored. In this chapter, we have tried to highlight the epigenetic mechanisms that play key roles in plants.

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“…An increase in cytosine methylation at CG sites was observed in rapeseed ( Brassica napus) under salt stress [5]. In tobacco ( Nicotiana tabacum), antisense silence of the DNA methyltransferase gene NtMET1 released a subset of stress-responsive genes, including a glycerophosphodiesterase-like gene, NtGP-DL [6].…”

Section: Epigenetic Changes In Plant Responses To Environmental Stressesmentioning

confidence: 99%

Role of Epigenetic Modifications in Plant Responses to Environmental Stresses

Liu¹,

Luo²,

Yang³

et al. 2015

Nuclear Functions in Plant Transcription, Signaling and Development

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Genetic and DNA-methylation changes induced by potassium dichromate in Brassica napus L.

Cited by 109 publications

References 39 publications

Vernalization and photoperiod-related changes in the DNA methylation state in winter and spring rapeseed

Vernalization and photoperiod-related changes in the DNA methylation state in winter and spring rapeseed

Epigenetic Mechanisms in Plants: An Overview

Role of Epigenetic Modifications in Plant Responses to Environmental Stresses

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