Genetic and Developmental Analyses of LDH Isozymes in Trout

Wright, James E.; Heckman, James R.; Atherton, Louisa M.

doi:10.1016/b978-0-12-472703-8.50030-5

Cited by 25 publications

(15 citation statements)

References 28 publications

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“…Similar associations between the initial expression of locus-specific LDH isozymes and developmental rates in three species of salmonid fishes have been reported [4].…”

Section: Discussionmentioning

confidence: 51%

Allelic differences in initial expression of paternal alleles at an isocitrate dehydrogenase locus in rainbow trout (Salmo gairdneri)

1984

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We examined a temporal series of embryos from 14 full-sib families of rainbow trout with starch gel electrophoresis to determine the time of initial detection of enzyme produced by Zdh-3. Maternal enzyme was detected in unfertilized eggs, whereas paternal alleles showed evidence of initial expression after gastrulation and epiboly. Two alleles, 40 and 71, were expressed synchronously several days before the 114 allele. Measurement of enzyme activity by spectrophotometric analysis and serial dilution supported these observations. The degree of delay of expression of the 114 allele between families was coupled with other estimates of developmental rate. These data suggest the existence of allelic variation at a cis-acting genetic element controlling the pattern of ontogenetic expression of structural alleles at Idh-3.

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“…Similar associations between the initial expression of locus-specific LDH isozymes and developmental rates in three species of salmonid fishes have been reported [4].…”

Section: Discussionmentioning

confidence: 51%

Allelic differences in initial expression of paternal alleles at an isocitrate dehydrogenase locus in rainbow trout (Salmo gairdneri)

1984

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“…The variant allele may encode a polypeptide with the same electrophoretic mobility as that encoded by the common allele at LDH-A2, or it could be a null (i.e., enzymatically inactive) allele. An analogous situation occurs 0t LDH-B1; this polymorphism was first described and shown to have a Mendelian basis in rainbow trout by Wright, Heckman and Atherton (1975).…”

Section: Null Allelesmentioning

confidence: 88%

“…LDH-AI and LDH-A2 are expressed only in skeletal muscle and are paralogous genes that are orthologous to the LDH-A locus of other vertebrates (Bailey & Wilson, 1968;Markert, Shaklee & Whitt, 1975). Similarly, the LDH-BI and LDH-B2 loci are paralogous duplicates arising from the orthologous LDH-B locus found in many groups of fish (Wright, Heckman & Atherton, 1975;Markert, Shaklee & Whitt, 1975). LDH-C is the only duplicate of the LDH-C locus (Markert, Shaklee & Whitt, 1975) that can still be detected.…”

Section: Introductionmentioning

confidence: 96%

Null alleles at two lactate dehydrogenase loci in rainbow trout are associated with decreased developmental stability

1993

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Previous studies with rainbow trout (Oncorhynchus mykiss) have shown that allozymic heterozygotes have increased developmental stability, as measured by reduced fluctuating bilateral asymmetry. In this paper, we examine the phenotypic effects of null alleles at two lactate dehydrogenase (LDH) loci. If the association between allozymic heterozygosity and developmental stability is due largely to linked chromosomal segments, then we would expect null allele heterozygotes to have increased developmental stability. In contrast, heterozygotes for LDH null alleles in three populations have reduced developmental stability. This suggests that the reduction in enzyme activity at these loci is having a deleterious effect on development that is strong enough to mask any beneficial effects that may be associated with heterozygosity for these chromosomal segments. The LDH loci examined in this study are members of two different paralogous pairs of duplicate genes produced by the polyploidization of the ancestral salmonid genome. The apparent deleterious effects of these null alleles in heterozygotes could retard the possible loss of duplicate gene expression.

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“…Progeny tests for Mdh-4 variants in the crosses between strains of the guppy isocitrate dehydrogense, a malic enzyme, a phosphoglucomutase, and an aspartate aminotransferase locus in brook trout (Stoneking et al, 1981;May et al, 1979), at an isocitrate dehydrogenase locus in Japanese char (Fujio et al, 1985), at a lactate dehydrogenase locus in rainbow trout (Wright et al, 1975), and at a malate dehydrogenase and a creatine kinase locus in brown trout (Allendorf et al, 1976). The existence of null allele polymorphism is of interest and importance in the process of gene loci duplication such as in the salmonid fishes which are thought to have been derived from an autotetraploid ancestor (Ohno, 1970;Allendorf et al, 1975;Gold, 1979).…”

Section: Discussionmentioning

confidence: 99%

Detection of a null allele for MDH isozymes in the guppy (Poecilia reticulata), with special reference to sex-linked inheritance.

Fujio

Macaranas

1989

The Japanese journal of genetics

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Malate dehydrogenase (MDH) was examined by starch gel electrophoresis for strain differences in the guppy, Poecilia reticulata.The electrophoretic patterns revealed duplicate gene loci encoding a mitochondrial form of MDH and the existence of a null allele at one of the duplicate loci.Different phenotypic frequencies at Mdh-4 were observed among 9 strains and between males and females within each strain. The progeny tests for Mdh-4 variants in the inter-strain crosses indicated a mode of partial sex-linked inheritance.

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Genetic and Developmental Analyses of LDH Isozymes in Trout

Cited by 25 publications

References 28 publications

Allelic differences in initial expression of paternal alleles at an isocitrate dehydrogenase locus in rainbow trout (Salmo gairdneri)

Allelic differences in initial expression of paternal alleles at an isocitrate dehydrogenase locus in rainbow trout (Salmo gairdneri)

Null alleles at two lactate dehydrogenase loci in rainbow trout are associated with decreased developmental stability

Detection of a null allele for MDH isozymes in the guppy (Poecilia reticulata), with special reference to sex-linked inheritance.

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