Genetic and Cytological Analyses of the Natural Variation of Seed Number per Pod in Rapeseed (Brassica napus L.)

Yang, Yuhua; Wang, Ying; Zhan, Jiasui; Shi, Jiaqin; Wang, Xinfa; Liu, Guihua; Wang, Hanzhong

doi:10.3389/fpls.2017.01890

Cited by 23 publications

(23 citation statements)

References 29 publications

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“…Previous studies have shown that the number of ovules per ovary, the proportion of fertile ovules, the proportion of fertile ovules to be fertilized, the proportion of fertilized ovules all determined the seed number per silique (Li et al, 2014;Li S. et al, 2015;Yang et al, 2017). The above experimental results showed that the number of ovules per ovary and the proportion of fertile ovules had no difference between NIL-Zhongshang11 and NIL-No.…”

Section: Cytological Analysis Of Qsna7mentioning

confidence: 54%

“…The previous studies have investigated the regulatory mechanisms of seed number per silique difference between the different cultivars, through cytological observation using several techniques. The results showed that ovule number and fertility, double fertilization and seed development all affect the final seed number per silique, although their relative contributions might differ in different cultivars (Li et al, 2014;Li S. et al, 2015;Yang et al, 2017).…”

Section: The Regulatory Mechanism Of the Seed Number Per Silique In Rmentioning

confidence: 94%

“…In our previous study, the 2nGoCGm model was adopted to reciprocal crosses experiments between four high-SNPS and five low-SNPS lines. The results showed that the maternal, embryo, and cytoplasm genotype respectively explained 47.6, 35.2, and 7.5% of the genetic variance (Yang et al, 2017).…”

Section: Introductionmentioning

confidence: 98%

“…In our previous study, the representative four high-SNPS and five low-SNPS lines were subjected artificial self-and reciprocal crosspollination experiment. The results showed that the seed number per silique of hybrid F 1 was mainly determined by the general combining ability (63%) of the parents, followed by the special combining ability (37%) of parental combination, which was mainly determined by the additive effect, followed by the dominant effect (Yang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…The cytological observation of Huashuang4, Zhongshuang11, and nine double haploid lines revealed that the difference of the seed number per silique in these lines was mainly due to the abortion of ovules (Li et al, 2014). Due to the complexity of seed number per silique, our previous study proposed the idea of decomposed it into four components: i.e., the number of ovules per ovary, the proportion of fertile ovules, the proportion of fertile ovules to be fertilized, the proportion of fertilized ovules to develop into seeds (Yang et al, 2017). The relative contributions of the four components were then studied using the representative four high-seed number per silique (SNPS) and five low-SNPS lines selected from a core association population (Li et al, 2019), which accounted for 30.7, 18.2, 7.1, and 43.9%, respectively (Yang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Validation and Characterization of a Seed Number Per Silique Quantitative Trait Locus qSN.A7 in Rapeseed (Brassica napus L.)

et al. 2020

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Seed number is a key character/trait tightly related to the plant fitness/evolution and crop domestication/improvement. The seed number per silique (SNPS) shows a huge variation from several to more than 30, however the underlying regulatory mechanisms are poorly known, which has hindered its improvement. To answer this question, several representative lines with extreme SNPS were previously subjected to systematic genetic and cytological analyses. The results showed that the natural variation of seed number per silique is mainly controlled by maternal and embryonic genotype, which are co-determined by ovule number per ovary, fertile ovule ratio, ovule fertilization rate, and fertilized ovule development rate. More importantly, we also mapped two repeatable quantitative trait loci (QTLs) for SNPS using the F 2:3 population derived from Zhongshuang11 and No. 73290, of which the major QTL qSN.A6 has been finemapped. In the current study, the near-isogenic lines (NILs) of qSN.A7 were successfully developed by the successive backcross of F 1 with Zhongshuang11. First, the effect of qSN.A7 was validated by evaluating the SNPS of two types of homozygous NILs from BC 3 F 2 population, which showed a significant difference of 2.23 on average. Then, qSN.A7 was successfully fine-mapped from the original 4.237 to 1.389 Mb, using a BC 4 F 2 segregating population of 2,551 individuals. To further clarify the regulatory mechanism of qSN.A7, the two types of homologous NILs were subjected to genetic and cytological analyses. The results showed that the difference in SNPS between the two homologous NILs was determined by the embryonic genotypic effect. Highly accordant with this, no significant difference was observed in ovule number per ovary, ovule fertility, fertilization rate, and pollen fertility between the two homologous NILs. Therefore, the regulatory mechanism of qSN.A7 is completely different from the cloned qSS.C9 and qSN.A6. These results will advance the understanding of SNPS and facilitate gene cloning and molecular breeding in Brassica napus.

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Section: Cytological Analysis Of Qsna7mentioning

confidence: 54%

Section: The Regulatory Mechanism Of the Seed Number Per Silique In Rmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Validation and Characterization of a Seed Number Per Silique Quantitative Trait Locus qSN.A7 in Rapeseed (Brassica napus L.)

et al. 2020

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Characterization of agronomic traits and genomic diversity in a newly assembled radish core collection

Huh,

Cho,

Yim

et al. 2023

Crop Science

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Crop breeding programs are reliant on the genetic diversity of varieties and germplasm collections. Raphanus sativus L. is a root crop species of the Brassicaceae family that accounts for approximately 2% of global vegetable production. We recently reported the assembly of a new radish core collection consisting of 125 accessions obtained from worldwide germplasm banks. To develop a comprehensive plant resource for radish research and breeding, we characterized the agronomic traits of 83 accessions and analyzed their genome‐wide DNA variations along with 17 previously analyzed accessions. A total of 14 agronomically important root, leaf, and flower phenotypic traits were examined according to the guidelines of the International Union for the Protection of New Plant Varieties. Chemical diversity in radish roots was investigated by measuring their sugar, glucosinolate, and anthocyanin contents. Through high‐depth whole‐genome resequencing of 100 accessions and multi‐sample genotyping analysis using the Rs2.0 genome assembly as a reference, we identified a total of 796,294 homozygous high‐quality single‐nucleotide polymorphisms. Genetic structural analysis using a neighbor‐joining algorithm and multi‐dimensional scaling analysis of the variations were applied to classify the accessions into three major groups, including 37 and 55 accessions of European and Asian cultivated radishes, respectively, and 8 wild radish accessions. For each accession, the mitochondria‐encoded Ogura cytoplasmic male‐sterility determinant orf138 and nuclear fertility restorer PPR‐B genes were reconstructed by reference‐guided assembly. It is anticipated that these genetic diversity and agronomic properties of the radish core collection will facilitate breeding programs to develop new radish cultivars with desirable profiles.This article is protected by copyright. All rights reserved

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Fine mapping and candidate gene analysis of a major locus controlling ovule abortion and seed number per silique in Brassica napus L.

et al. 2021

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Key message A major QTL controlling ovule abortion and SN was ne-mapped to a 80.1-kb region on A8 in rapeseed, and BnaA08g07940D and BnaA08g07950D are the most likely candidate genes.Abstact The seed number per silique (SN), an important yield determining trait of rapeseed, is the nal consequence of a complex developmental process including ovule initiation and the subsequent ovule/seed development. To elucidate the genetic mechanism regulating the natural variation of SN and its related components, quantitative trait locus (QTL) mapping was conducted using a doubled haploid (DH) population derived from the cross between C4-146 and C4-58B, which showed significant differences in SN and aborted ovule number (AON), but no obvious differences in ovule number (ON). QTL analysis identified 19 consensus QTLs for six SN-related traits across three environments. A novel QTL on chromosome A8, un.A8, which pleiotropically controls all these traits except for ON, was stably detected across the three environments.This QTL explained more than 50% of the SN, AON and percentage of aborted ovule (PAO) variations as well as a moderate contribution on silique length (SL) and thousand seed weight (TSW). The C4-146 allele at the locus increases SN and SL but decreases AON, PAO and TSW. Further ne mapping narrowed down this locus into a 80.1-kb interval anked by markers BM1668 and BM1672, and six predicted genes were annotated in the delimited region. Expression analyses and DNA sequencing showed that two homologs of Arabidopsis photosystem I subunit F (BnaA08g07940D) and zinc transporter 10 precursor (BnaA08g07950D) were the most promising candidate genes underlying this locus. These results provide a solid basis for cloning un.A8 to reduce the ovule abortion and increase SN in the yield improvement of rapeseed.

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Genetic and Cytological Analyses of the Natural Variation of Seed Number per Pod in Rapeseed (Brassica napus L.)

Cited by 23 publications

References 29 publications

Validation and Characterization of a Seed Number Per Silique Quantitative Trait Locus qSN.A7 in Rapeseed (Brassica napus L.)

Validation and Characterization of a Seed Number Per Silique Quantitative Trait Locus qSN.A7 in Rapeseed (Brassica napus L.)

Characterization of agronomic traits and genomic diversity in a newly assembled radish core collection

Fine mapping and candidate gene analysis of a major locus controlling ovule abortion and seed number per silique in Brassica napus L.

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