Genetic and biological characterization of avian influenza H5N1 viruses isolated from wild birds and poultry in Western Siberia

Sharshov, Kirill; Romanovskaya, Alesia; Uzhachenko, Roman V.; Durymanov, A. G.; Zaykovskaya, А. V.; Kurskaya, Olga; Ilinykh, Philipp A.; Silko, Nikita Y.; Kulak, Mikhail V.; Alekseev, Alexander; Zolotykh, S. I.; Shestopalov, А. М.; Drozdov, I. G.

doi:10.1007/s00705-010-0676-2

Cited by 25 publications

(17 citation statements)

References 31 publications

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“…We collected data from participants via interview regarding their exposures to wild birds and performed serologic testing for antibodies against a clade 2.2 HPAI H5N1 virus strain. Clade 2.2 is the HPAI H5N1 virus clade that has spread to regions outside of Asia since 2005, with documented infection of wild birds as far east as Western Siberia …”

Section: Methodsmentioning

confidence: 99%

Characterizing wild bird contact and seropositivity to highly pathogenic avian influenza A (H5N1) virus in Alaskan residents

Reed

Bruden

Byrd

et al. 2014

Influenza Resp Viruses

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BackgroundHighly pathogenic avian influenza A (HPAI) H5N1 viruses have infected poultry and wild birds on three continents with more than 600 reported human cases (59% mortality) since 2003. Wild aquatic birds are the natural reservoir for avian influenza A viruses, and migratory birds have been documented with HPAI H5N1 virus infection. Since 2005, clade 2.2 HPAI H5N1 viruses have spread from Asia to many countries.ObjectivesWe conducted a cross-sectional seroepidemiological survey in Anchorage and western Alaska to identify possible behaviors associated with migratory bird exposure and measure seropositivity to HPAI H5N1.MethodsWe enrolled rural subsistence bird hunters and their families, urban sport hunters, wildlife biologists, and a comparison group without bird contact. We interviewed participants regarding their exposures to wild birds and collected blood to perform serologic testing for antibodies against a clade 2.2 HPAI H5N1 virus strain.ResultsHunters and wildlife biologists reported exposures to wild migratory birds that may confer risk of infection with avian influenza A viruses, although none of the 916 participants had evidence of seropositivity to HPAI H5N1.ConclusionsWe characterized wild bird contact among Alaskans and behaviors that may influence risk of infection with avian influenza A viruses. Such knowledge can inform surveillance and risk communication surrounding HPAI H5N1 and other influenza viruses in a population with exposure to wild birds at a crossroads of intercontinental migratory flyways.

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Section: Methodsmentioning

confidence: 99%

Characterizing wild bird contact and seropositivity to highly pathogenic avian influenza A (H5N1) virus in Alaskan residents

Reed

Bruden

Byrd

et al. 2014

Influenza Resp Viruses

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“…The West Siberian Plain is a territory of particular importance (Gilbert et al 2006;Sharshov et al 2007Sharshov et al , 2010Donchenko et al 2010) as there are a lot of rivers and lakes in the south of Western Siberia that are situated on migratory routes and serve as breeding grounds for many species that are ecologically connected with water bodies. Lake Chany is the biggest lake of the region and is known to be the site of the largest migratory stopover of birds (Veen et al 2005).…”

Section: Open Accessmentioning

confidence: 99%

Avian influenza virus ecology in wild birds of Western Siberia

Sharshov

Yurlov

et al. 2017

Avian Res

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Background: The aim of the study was to explore the ecological diversity of wild birds in Siberia, which are the natural reservoir of avian influenza virus (AIV). Methods:Cloacal swabs and intestinal fragments were collected from wild migratory birds from 2007-2014. Isolated viruses were grown in the allantoic cavity of embryonated chicken eggs. The presence of virus was determined using hemagglutination assays. Primary identification and subtyping of influenza viruses was confirmed by RT-PCR. Conclusions:Wild migratory waterfowl of orders Anseriformes and Charadriiformes are the main reservoir of AIV in the south of Western Siberia. This area plays a key role in persistence, evolution, and geographical distribution of avian influenza.

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“…Recent outbreaks of an avian-origin H5N8, H7N9 influenza virus raise concern of the emergence of novel reassortant viruses in Eurasia and the potential threat to the human population [2] [3]. The highly pathogenic influenza A virus subtype H5N1 (HPAI H5N1) spread throughout Asia since 2003, reached to Europe in 2005, and the Middle East, as well as Africa and caused a global concern for a potential pandemic threat last decade [4] [5] [6] [7]. In 2005, a great amount of migratory birds were infected by HPAI H5N1 in Qinghai Lake region (northwest of China) which resulted the death of over 6000 birds [4] [6] [8].…”

Section: Introductionmentioning

confidence: 99%

The Evidence of Clade 7.1 Avian Influenza Virus (H5N1) in Qinghai Lake

Wang¹,

Sharshov²,

Li³

et al. 2016

AiM

Self Cite

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The highly pathogenic influenza A virus subtype H5N1 spread throughout Asia since 2003, reached to Europe in 2005, and the Middle East, as well as Africa and caused a global concern for a potential pandemic threat last decade. A Clade 2.3.2 H5N1 virus became dominate in the Qinghai Lake region in 2009 with sporadic mammal cases of infection and transferred to Russia and Europe through wild migratory birds. Currently, HPAI H5N1 of clades 2.3.4, 2.3.2, and 7 are the dominant co-circulating H5N1 viruses in poultry in Asia. 2.3.2 Clade is dominant in wild birds through the world whereas there is no evident data about Clade 7 circulation in wild birds. We detected HPAI H5N1 virus of Clade 7.1 in Qinghai Lake, that closely related to Shanxi-like and Vietnam viruses co-circulating in poultry. This is the first report of Clade 7.1 H5N1 in wild birds. Based on phylogenetic analyses, the virus can be originated from Clade 7.1 virus gene pool that spread in Vietnam and Chinese poultry and could spread with migratory birds to Qinghai Lake. The Qinghai Lake continues to be significant hotspot for H5N1 surveillance since the regular outbreaks occurred there in wild birds and mammals. Based on these facts and findings, the related researchers should pay more attention to the Qinghai Lake basin as significant hotspot for H5N1 avian influenza surveillance since the regular H5N1 outbreaks occurred there in wild birds with sporadic mammal cases of infection.

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Genetic and biological characterization of avian influenza H5N1 viruses isolated from wild birds and poultry in Western Siberia

Cited by 25 publications

References 31 publications

Characterizing wild bird contact and seropositivity to highly pathogenic avian influenza A (H5N1) virus in Alaskan residents

Characterizing wild bird contact and seropositivity to highly pathogenic avian influenza A (H5N1) virus in Alaskan residents

Avian influenza virus ecology in wild birds of Western Siberia

The Evidence of Clade 7.1 Avian Influenza Virus (H5N1) in Qinghai Lake

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