Genetic and Antigenic Variability of Human Respiratory Syncytial Virus (Groups A and B) Isolated over Seven Consecutive Seasons in Argentina (1995 to 2001)

Galiano, Mónica; Palomo, Concepción; Videla, Cristina; Arbiza, Juan; Melero, José A.; Carballal, Guadalupe

doi:10.1128/jcm.43.5.2266-2273.2005

Cited by 50 publications

(61 citation statements)

References 45 publications

(70 reference statements)

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“…A total of 353 of those samples were positive for HRSV and were classified in antigenic group A or B by multiplex nested RT-PCR of the G protein gene, as described by Stockton et al (44). As found in other studies done in Argentina (16,52), group A sequences were detected more frequently than group B sequences throughout the study period, although group B samples were more abundant than group A sequences in 1999 and 2002 (Table 1). HRSV group a Number of samples that tested positive by multiplex nested RT-PCR for HRSV.…”

Section: Resultssupporting

confidence: 83%

Natural History of Human Respiratory Syncytial Virus Inferred from Phylogenetic Analysis of the Attachment (G) Glycoprotein with a 60-Nucleotide Duplication

Trento

Viegas

Galiano

et al. 2006

J Virol

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158

172

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. Phylogenetic analysis indicated that BA sequences with that duplication shared a common ancestor (dated about 1998) with other HRSV G sequences reported worldwide after 1999. The duplicated nucleotide sequence was an exact copy of the preceding 60 nucleotides in early viruses, but both copies of the duplicated segment accumulated nucleotide substitutions in more recent viruses at a rate apparently higher than in other regions of the G protein gene. The evolution of the viruses with the duplicated G segment apparently followed the overall evolutionary pattern previously described for HRSV, and this genotype has replaced other prevailing antigenic group B genotypes in Buenos Aires and other places. Thus, the duplicated segment represents a natural tag that can be used to track the dissemination and evolution of HRSV in an unprecedented setting. We have taken advantage of this situation to reexamine the molecular epidemiology of HRSV and to explore the natural history of this important human pathogen.

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Section: Resultssupporting

confidence: 83%

Natural History of Human Respiratory Syncytial Virus Inferred from Phylogenetic Analysis of the Attachment (G) Glycoprotein with a 60-Nucleotide Duplication

Trento

Viegas

Galiano

et al. 2006

J Virol

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158

172

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“…The predominant genotypes were A5, A2 and B3, and these also represented the most frequently encountered in other regions of the world, such as in South Africa, Uruguay and Argentina. [22][23][24] The association of immunofluorescence and culture for RSV rendered high sensitivity to the diagnostic method. Although the possibility exists of false negative results from our method which might be identified by the use of RT-PCR, the known difference in sensitivity between these methods does not suggest that the interpretations of our results or the conclusions of this study would be altered with the use of RT-PCR.…”

Section: Discussionmentioning

confidence: 99%

Lower Respiratory Tract Infection Caused by Respiratory Syncytial Virus in Infants: The Role Played by Specific Antibodies

Vieira¹,

Gilio

Durigon

et al. 2007

Clinics

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Vieira SE, Gilio AE, Durigon EL, B Ejzenberg. Lower respiratory tract infection caused by respiratory syncytial virus in infants: the role played by specific antibodies. Clinics. 2007; 62(6):709-16. INTRODUCTION:Respiratory syncytial virus (RSV) is a major etiological agent of lower respiratory tract infection in infants. Genotypes of this virus and the role of the infants' serum antibodies have yet to be fully clarified. This knowledge is important for the development of effective therapeutic and prophylactic measures. OBJECTIVES: To evaluate the types and genotypes of RSV causing respiratory tract infection in infants, to analyze the association of subtype-specific serum antibodies with the occurrence of infection and to evaluate the presence of subtype-specific antibodies in the infants' mothers and their association with the profile of the childrens' serum antibodies. METHODS:This was a prospective study on infants hospitalized with respiratory infection. Nasopharyngeal secretions were collected for viral investigation using indirect immunofluorescence and viral culture and blood was collected to test for antibodies using the Luminex Multiplex system. RESULTS: 192 infants were evaluated, with 60.9% having RSV (73.5%-A and 20.5% B). Six genotypes of the virus were identified: A5, A2, B3, B5, A7 and B4. The seroprevalence of the subtype-specific serum antibodies was high. The presence and levels of subtype-specific antibodies were similar, irrespective of the presence of infection or the viral type or genotype. The mothers' antibody profiles were similar to their infants'. CONCLUSIONS: Although the prevalence of subtype-specific antibodies was elevated, these antibodies did not provide protection independently of virus type/genotype. The similarity in the profiles of subtype-specific antibodies presented by the mothers and their children was consistent with transplacental passage.

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“…In all 12 patients, sequences from the sequential sampling were identical and no genetic variation in the N-terminal region of the G gene could be demonstrated during the phase of illness. International data from molecular epidemiological studies of RSV are derived from long-term studies with 100 or more isolates [Peret et al, 1998;Choi and Lee, 2000;Seki et al, 2001;Venter et al, 2001;Scott et al, 2004;Kuroiwa et al, 2005;Sato et al, 2005;Viegas and Mistchenko, 2005], from long-term studies with a more limited number if isolates [Cane et al, 1994;Garcia et al, 1994;Zambon et al, 2001;Venter et al, 2002;Frabasile et al, 2003;Rafiefard et al, 2004;Galiano et al, 2005;Parveen et al, 2006], and also from shortterm studies like this one from Stockholm [Peret et al, 2000;Moura et al, 2004]. This study provides data from a complete season.…”

Section: Genetic Diversity Of Rsv Strains Found In Consecutive Samplementioning

confidence: 99%

“…The epidemiology and the genetic diversity of RSV have been studied in many parts of the world, among them, the Gambia [Cane et al, 1999], Korea [Choi and Lee, 2000], Uruguay and Argentina [Frabasile et al, 2003], Argentina [Galiano et al, 2005], Japan [Kuroiwa et al, 2005;Sato et al, 2005], South Africa [Venter et al, 2001;Venter et al, 2002;Madhi et al, 2003], Brazil [Moura et al, 2004], India [Parveen et al, 2006], USA [Peret et al, 1998;Peret et al, 2000], Kenya [Scott et al, 2004], Great Britain [Zambon et al, 2001], and Belgium [Zlateva et al, 2005]. At least three studies from Europe have been published during the past decade.…”

Section: Genetic Diversity Of Rsv Strains Found In Consecutive Samplementioning

confidence: 99%

Molecular epidemiology and genetic variability of respiratory syncytial virus (RSV) in Stockholm, 2002–2003

Östlund¹,

Lindell²,

Stenler³

et al. 2007

Journal of Medical Virology

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The epidemiology and genetic variability of circulating respiratory syncytial virus (RSV) strains in Stockholm during the season 2002-2003 were studied in consecutive RSV isolates derived from respiratory samples and diagnosed in the laboratory. Two hundred thirty-four viruses were sequenced. The samples were mainly from children under 1 year old (79%). The phylogeny of the N-terminal part of the G gene was studied after amplification and sequencing. One hundred fifty-two viruses belonged to subgroup B and 82 to subgroup A. The subgroup A viruses could be further divided into genotypes GA2 (25) and GA5 (57) and the subgroup B viruses into GB3 (137) and SAB1 (15) strains. These strains clustered with subgroup A and subgroup B strains from Kenya from the same period, as well as with strains from Great Britain from 1995 to 1998. The dominance of subgroup B strains in Stockholm during 2002-2003 is in agreement with findings from other parts of the world during the same years. Only two genotypes of subgroup A, GA2 and GA5, were circulating during this time, and GA2 has been circulating in Sweden for more than 20 years. Consecutive strains from the same individual displayed no variability in the sequenced region, which was also true of strains that had been passaged in cell cultures.

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Genetic and Antigenic Variability of Human Respiratory Syncytial Virus (Groups A and B) Isolated over Seven Consecutive Seasons in Argentina (1995 to 2001)

Abstract: The genetic and antigenic variability of human respiratory syncytial virus (HRSV) strains isolated in Buenos

Cited by 50 publications

References 45 publications

Natural History of Human Respiratory Syncytial Virus Inferred from Phylogenetic Analysis of the Attachment (G) Glycoprotein with a 60-Nucleotide Duplication

Natural History of Human Respiratory Syncytial Virus Inferred from Phylogenetic Analysis of the Attachment (G) Glycoprotein with a 60-Nucleotide Duplication

Lower Respiratory Tract Infection Caused by Respiratory Syncytial Virus in Infants: The Role Played by Specific Antibodies

Molecular epidemiology and genetic variability of respiratory syncytial virus (RSV) in Stockholm, 2002–2003

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