Genetic Analysis on Hibiscus Species by Using Rapd Markers

Abstract

This study was undertaken for genetic analysis within the 7 varieties of Hibiscus rosa-sinensis by using RAPD markers the method involves a extraction of DNA from leaves of Hibiscus rosa-sinensis and yield of DNA ranged from 158-200µg and purity (ratio) was between 1.3-1.6 indicating minimum level of contaminating metabolites. Genetic analysis and relationship among seven Hibiscus rosasinensis germplasm were analyzed using Random Amplified Polymorphic DNA (RAPD). Total nine primers were used as, RPI-07, RPI-08, RPI-10, RPI-12, RPI-18, RPI-19, RPI-21, RPI-22 and RPI-23. No PCR product was generated through the primer RPI-23 indicating that no binding sites for this primer. In all the primers RPI-07, RPI-08, RPI-10, RPI-19 and RPI-21 are more suitable for the genetic variation analysis in Hibiscus species as it has shown maximum polymorphic bands. Primers RPI-12, RPI-18 and RPI-22 are weak in showing the polymorphic banding pattern for the all species of Hibiscus rosa-sinensis. By using PAST and MEGA programme Consensus Phylogenetic tree generated based on genetic distances segregated the seven Hibiscus germplasm into two different clusters. 75% times of species Hibiscus rosa-sinensis Red S.P. and D.P. (Single petal, Scarlet and Double petal, Rubroplenus) clustered in a single cluster. 87% times the cluster of Hibiscus rosasinensis Pink varieties clustered with Hibiscus rosa-sinensis White varieties. The results of the present study indicated that the RAPD analysis could be utilized by breeders for further improvement of Hibiscus rosa-sinensis species.

Valencia ve Navel grup portakal çeşitlerinin SSR markörleri yardımıyla genetik çeşitlilik analizi

“…In later years, PAST has grown into a comprehensive statistics package that is used not only by paleontologists, but also in many fields of life science, earth science, and even engineering and economics (Hammer et al, 2001). PAST program was used in DNA fingerprinting (ISSR and RAPD) of Prosopis cineraria and P.juliflora (Elmeer and Almalki, 2011), molecular (SSR) analysis of old apple cultivars (Király et al, 2012), genetic analysis (RAPD) of Hibiscus species (Kadve et al, 2012), molecular (SSR) determination of genetic structure of Brazilian soybean cultivars (Piriolli et al, 2013), SSR-based genetic diversity assessment in tetraploid and hexaploid wheat populations (Abouzied et al, 2013), molecular diversity (SSR) in cultivated groundnut (Goswami et al, 2013). …”

Does Matk, Trnh-Psba, Trnl-F And Its1 Identification Mirror Morphological Identification In Hibisceae?

“…The difficulties and arising errors in morphological identification and taxonomy may be overcome by combining it with molecular identification (Batovska et al, 2016). While molecular based studies (RAPD-PCR, ISSR-PCR or AFLP) have been used in identifying Hibiscus species (Kadve et al, 2012;Khafaga, 2013;Omalsaad et al, 2014;Tang et al, 2003), DNA barcoding has not been widely used. Loci such as atpB, rbcL, and ndhF, have focused on phylogeny at higher taxonomic levels (Pfeil et al, 2002;Pfeil & Crisp, 2005;Tate & Simpson, 2003).…”

Diversity Analysis of 15 Hibiscus Accession Based on RAPD Marker

“…It has great variation of morphological characters such as flower colors and sizes, and the leaf shapes. The great variation in Hibiscus rosa-sinensis was observed because of crosses and mutation (Kadve et al, 2012). Another variation is observed in the flower shape either single and double petals (Hammad, 2009).…”

“…One of the most used molecular marker is Random Amplified Polymorphic DNA (RAPD). RAPD is easy and uses random primer DNA sequences as marker (Kadve et al, 2012). RAPD usually uses 10 bases short primer sequences to randomly amplify DNA genom.…”