Genetic Analysis on Floury Endosperm Characteristics of 'Namil(SA)-flo1', a Japonica Rice Mutant Line

모영준,; 정지웅,; 강경호,; 이점식,; 김보경,

doi:10.7740/kjcs.2013.58.3.283

Cited by 4 publications

(6 citation statements)

References 14 publications

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“…Electron microscope visualization of the transverse sections of the Namsil(SA)-flo1 and wild-type mature endosperms showed that the Namsil(SA)-flo1 mutant was loosely packed with irregular and round-shaped compound starch granules, while the wild type contained densely packed polyhedral starch granules (Figure 1b). However, the physicochemical characteristics of Namsil(SA)-flo1 were different from those reported for flo4-1, flo4-2, and flo4-3, which were generated via T-DNA insertion into the cyOsPPDK gene [15,33,34]. We speculate that the cyOsPPDK gene may have diverse roles in the rice seed filling stage.…”

Section: Discussionmentioning

confidence: 86%

“…Physicochemical analysis of the Suweon 542 endosperm revealed a loosely packed structure of irregular and globular-shaped starch granules, a low protein content and grain weight, and high amylose content compared with its wild-type progenitor, Namil. During dry milling, Suweon 542 had significantly lower grain hardness, finer particle size, more loosely packed starch granules, and lower starch damage than Namil and other rice cultivars [33]. Genetic analysis of the floury endosperm characteristics of Suweon 542 revealed that the location of the target gene was in the 19.33-19.73 Mbp region on chromosome 5 between markers RM18624 and RM18639, and map-based cloning revealed a G→A SNP in exon 8 of cyOsPPDK (flo4-4), which was responsible for the floury endosperm of Suweon 542 [33,34].…”

Section: Introductionmentioning

confidence: 86%

“…During dry milling, Suweon 542 had significantly lower grain hardness, finer particle size, more loosely packed starch granules, and lower starch damage than Namil and other rice cultivars [33]. Genetic analysis of the floury endosperm characteristics of Suweon 542 revealed that the location of the target gene was in the 19.33-19.73 Mbp region on chromosome 5 between markers RM18624 and RM18639, and map-based cloning revealed a G→A SNP in exon 8 of cyOsPPDK (flo4-4), which was responsible for the floury endosperm of Suweon 542 [33,34]. Moreover, the floury endosperm of Namil(SA)-flo1 controlled by one recessive gene and the locus of Namil(SA)-flo1 was localized to the 17.7-20.7 Mbp region of chromosome 5 [35].…”

Section: Introductionmentioning

confidence: 86%

“…In a previous study, the F2 population of Namil(SA)-flo1/Milyang 23 (non-waxy Tongil cultivar) was used for genetic analysis [33]. To investigate the candidate genomic region for FLO4-5, F 2 lines heterozygous for the genomic region between RM18624 and RM18639 were grown to the F 3 generation.…”

Section: Fine Mapping Of Flo4-5mentioning

confidence: 99%

“…The Namil(SA)-flo1 locus was previously refined to a region on chromosome 5 and, alongside FLO4-4 (identified from Namil(SA)-flo2, also known as Suweon 542), was linked to chromosome 5 markers RM18624 and RM18639 [33,35]. To map the Namil(SA)-flo1 target gene more precisely, the whole genomes of Namil(SA)-flo1 and Milyang 23 were re-sequenced and aligned against one another to select homologous SNPs for developing CAPS markers.…”

Section: Fine Mapping Of the Floury Endosperm Locusmentioning

confidence: 99%

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A New SNP in Rice Gene Encoding Pyruvate Phosphate Dikinase (PPDK) Associated with Floury Endosperm

Wang

Ham

et al. 2020

Genes

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Rice varieties with suitable flour-making qualities are required to promote the rice processed-food industry and to boost rice consumption. A rice mutation, Namil(SA)-flo1, produces grains with floury endosperm. Overall, grains with low grain hardness, low starch damage, and fine particle size are more suitable for use in flour processing grains with waxy, dull endosperm with normal grain hardness and a high amylose content. In this study, fine mapping found a C to T single nucleotide polymorphism (SNP) in exon 2 of the gene encoding cytosolic pyruvate phosphate dikinase (cyOsPPDK). The SNP resulted in a change of serine to phenylalanine acid at amino acid position 101. The gene was named FLOURY ENDOSPERM 4-5 (FLO4-5). Co-segregation analysis with the developed cleaved amplified polymorphic sequence (CAPS) markers revealed co-segregation between the floury phenotype and the flo4-5. This CAPS marker could be applied directly for marker-assisted selection. Real-time RT-PCR experiments revealed that PPDK was expressed at considerably higher levels in the flo4-5 mutant than in the wild type during the grain filling stage. Plastid ADP-glucose pyrophosphorylase small subunit (AGPS2a and AGPS2b) and soluble starch synthase (SSIIb and SSIIc) also exhibited enhanced expression in the flo4-5 mutant.

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Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 86%

Section: Fine Mapping Of Flo4-5mentioning

confidence: 99%

Section: Fine Mapping Of the Floury Endosperm Locusmentioning

confidence: 99%

See 3 more Smart Citations

A New SNP in Rice Gene Encoding Pyruvate Phosphate Dikinase (PPDK) Associated with Floury Endosperm

Wang

Ham

et al. 2020

Genes

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Characterization of Physicochemical Properties of Starch in Barley Irradiated with Proton Beam

Kim¹,

Park²,

Kim

2013

Korean Journal of Crop Science

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The study was carried out to determine the gel pasting properties of barley (Hordeum vulgare L. cv. Geoncheonheugbori) as affected by different proton beam irradiation. The λmax, blue value, and amylose content were significantly associated with increasing proton beam irradiation. The pasting time in barley flour irradiated with proton beam ranged 0.09 to 0.16 min shorter than nonirradiated barley flour. Gel pasting temperature ranged 57.4 to 60.5℃. Gel pasting temperature in barley flour decreased with increasing proton beam irradiation. Proton beam irradiation caused a significant decrease in the onset temperature (To), peak temperature (Tp), conclusion temperature (Tc) and enthalpy change (ΔH). Gelatinization range (R) in barley starch was more broaden than that of non-irradiated barley starch. Barley starches gave the strong diffraction peak at around 2θ values15°, 18°, 20°, and 23° 2θ. Peak intensity tended to increase with increased proton beam irradiation. The granule crystallinity is closely associated with decreased amylose and increased amylopectin component. The crystallinity degree of barley starch irradiated with proton beam was significantly increased and it ranged from 24.9 to 32.9% compared to the non-irradiated barley starches. It might be deduced that proton beam irradiation causes significant changes of properties of starch viscosity in rice, especially at high irradiation of proton beam.

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Agronomic and Genetic Evaluation on a Dull Mutant Line Derived from the Sodium Azide Treated ‘Namil’, a Non-Glutinous Japonica Rice

Chun¹,

Jeung²,

Cho³

et al. 2015

Korean J. Crop Sci.

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Genetic Analysis on Floury Endosperm Characteristics of 'Namil(SA)-flo1', a Japonica Rice Mutant Line

Cited by 4 publications

References 14 publications

A New SNP in Rice Gene Encoding Pyruvate Phosphate Dikinase (PPDK) Associated with Floury Endosperm

A New SNP in Rice Gene Encoding Pyruvate Phosphate Dikinase (PPDK) Associated with Floury Endosperm

Characterization of Physicochemical Properties of Starch in Barley Irradiated with Proton Beam

Agronomic and Genetic Evaluation on a Dull Mutant Line Derived from the Sodium Azide Treated ‘Namil’, a Non-Glutinous Japonica Rice

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