Genetic analysis of wild-type Dobrava hantavirus in Slovenia: co-existence of two distinct genetic lineages within the same natural focus

Avšič–Županc, Tatjana; Nemirov, Kirill; Petrovec, Miro; Trilar, Tomi; Poljak, Mario; Vaheri, Antti; Plyusnin, Alexander

doi:10.1099/0022-1317-81-7-1747

Cited by 78 publications

(62 citation statements)

References 28 publications

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“…Nested PCR assays were used to detect the presence of hantaviruses (PUUV, DOBV, and TULV). [24][25][26] All primer sequences are listed in Table 1. PCR products were size-fractionated in 1% agarose gels and visualized using ethidium bromide before purification for dideoxy sequencing.…”

Section: Methodsmentioning

confidence: 99%

Molecular Survey of Zoonotic Agents in Rodents and Other Small Mammals in Croatia

Tadin¹,

Tokarz²,

Markotić³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Croatia is a focus for many rodent-borne zoonosis. Here, we report a survey of 242 rodents and small mammals, including 43 Myodes glareolus, 131 Apodemus flavicollis, 53 Apodemus agrarius, three Apodemus sylvaticus, six Sorex araneus, four Microtus arvalis, one Microtus agrestis, and one Muscardinus avellanarius, collected at eight sites in Croatia over an 8-year period. Multiplex MassTag polymerase chain reaction (PCR) was used for detection of Borrelia, Rickettsia, Bartonella, Babesia, Ehrlichia, Anaplasma, Francisella tularensis, and Coxiella burnetii. Individual PCR assays were used for detection of Leptospira, lymphocytic choriomeningitis virus, orthopoxviruses, flaviviruses, hantaviruses, and Toxoplasma gondii. Of the rodents, 52 (21.5%) were infected with Leptospira, 9 (3.7%) with Borrelia miyamotoi, 5 (2%) with Borrelia afzelii, 29 (12.0%) with Bartonella, 8 (3.3%) with Babesia microti, 2 (0.8%) with Ehrlichia, 4 (1.7%) with Anaplasma, 2 (0.8%) with F. tularensis, 43 (17.8%) with hantaviruses, and 1 (0.4%) with an orthopoxvirus. Other agents were not detected. Multiple infections were found in 32 rodents (13.2%): dual infections in 26 rodents (10.7%), triple infections in four rodents (2.9%), and quadruple infections in two rodents (0.8%). Our findings indicate that rodents in Croatia harbor a wide range of bacteria and viruses that are pathogenic to humans.

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Section: Methodsmentioning

confidence: 99%

Molecular Survey of Zoonotic Agents in Rodents and Other Small Mammals in Croatia

Tadin¹,

Tokarz²,

Markotić³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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“…Although a large overlap between the distribution of A. agrarius and A. flavicollis exists in Europe, so far the two hostspecific subtypes have been detected sympatrically only in the border region between central and southeastern Europe (3,23). The coexistence of two different virus lineages in the same geographic region indicates some genetic stability and evolutionary distinctness.…”

mentioning

confidence: 99%

Genetic Interaction between Distinct Dobrava Hantavirus Subtypes in Apodemus agrarius and A. flavicollis in Nature

Klempa

Schmidt

Ulrich

et al. 2003

J Virol

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Dobrava virus (DOBV) occurs in two different rodent species, Apodemus flavicollis (DOBV-Af) and A. agrarius (DOBV-Aa). We sequenced the S and M genomic segments from sympatric DOBV-Af and DOBV-Aa strains which fell into two distinct genetic lineages. Molecular phylogenetic analyses gave evidence for genetic reassortment between S and M segments of DOBV-Af and DOBV-Aa and indicated homologous recombination events in DOBV evolution. DOBV-Af and DOBV-Aa are distinct but also subject to genetic exchanges that affect their evolutionary trajectories.

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“…Moreover, a second DOBV cell culture isolate (Ano-Poroia or AP/Af) has been established from an A. flavicollis mouse trapped in Greece (32,34). Molecular phylogenetic analyses of nucleotide sequences from these cell culture-derived virus isolates, as well as from further South-East European A. flavicollis-associated strains, demonstrated their close mutual relationship (2,4,34,35). These strains form a unique A. flavicollisassociated DOBV clade, DOBV-Af (15).…”

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confidence: 99%

Central European Dobrava Hantavirus Isolate from a Striped Field Mouse ( Apodemus agrarius )

et al. 2005

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Dobrava virus (DOBV) is a hantavirus that causes hemorrhagic fever with renal syndrome (HFRS) inEurope. It is hosted by at least two rodent species, Apodemus flavicollis and A. agrarius. According to their natural hosts they form the distinct genetic lineages DOBV-Af and DOBV-Aa, respectively. We have now established a DOBV isolate named Slovakia (SK/Aa) from an A. agrarius animal captured in Slovakia. The complete S and M and partial L segment nucleotide sequences of the new isolate were determined. Phylogenetic analyses showed that the SK/Aa isolate clustered together with the other DOBV-Aa sequences amplified from A. agrarius before and can be taken as the representative of this genetic lineage. SK/Aa, in comparison with a DOBV-Af isolate, was used for serotyping neutralizing antibodies of HFRS patients in Central Europe. Most patients' sera exhibited a higher endpoint titer when probed with our new isolate, suggesting that DOBV-Aa strains are responsible for most of the DOBV-caused HFRS cases in this region.Hantaviruses represent a unique genus Hantavirus within the Bunyaviridae family. They are "emerging viruses" that cause two human zoonoses: hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome. The virus genome consists of three segments of negative-stranded RNA; the large (L) segment encodes the viral RNA-dependent RNA polymerase, the medium (M) segment the glycoprotein precursor (GPC), and the small (S) segment of the nucleocapsid (N) protein. In contrast to other genera of the Bunyaviridae family, hantaviruses are not transmitted by arthropods. Instead, they are spread by aerosolized rodent excreta and produce a chronic infection with no apparent harm in their natural hosts, rodents of the family Muridae (18,28,45,46). In Europe, at least two hantaviruses, Dobrava virus (DOBV) and Puumala virus (PUUV), are known to be pathogenic for humans (for reviews, see references 18 and 37). In addition, a single case of renal syndrome with pulmonary involvement has been very recently associated with infection by Tula virus (14). Currently, DOBV is intensively studied because of its unique properties; different virus lineages exist in different regions of Europe, and these lineages are harbored by different host reservoirs and probably display different levels of virulence in humans.A viable DOBV strain was isolated from lungs of a yellownecked mouse (Apodemus flavicollis) captured in a natural focus of HFRS in Dobrava village, Slovenia, South-East Europe (5). This virus isolate represents the DOBV prototype strain (named Slovenia or Slo/Af here) from A. flavicollis. Moreover, a second DOBV cell culture isolate (Ano-Poroia or AP/Af) has been established from an A. flavicollis mouse trapped in Greece (32, 34). Molecular phylogenetic analyses of nucleotide sequences from these cell culture-derived virus isolates, as well as from further South-East European A. flavicollis-associated strains, demonstrated their close mutual relationship (2, 4, 34, 35). These strains form a unique A. fla...

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Genetic analysis of wild-type Dobrava hantavirus in Slovenia: co-existence of two distinct genetic lineages within the same natural focus

Cited by 78 publications

References 28 publications

Molecular Survey of Zoonotic Agents in Rodents and Other Small Mammals in Croatia

Molecular Survey of Zoonotic Agents in Rodents and Other Small Mammals in Croatia

Genetic Interaction between Distinct Dobrava Hantavirus Subtypes in Apodemus agrarius and A. flavicollis in Nature

Central European Dobrava Hantavirus Isolate from a Striped Field Mouse ( Apodemus agrarius )

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