Genetic analysis of transgenome structure and size of chromosome-mediated gene transfer lines

Abstract

The TK-selected chromosome-mediate gene transfer lines were analysed using DNA dot blot method, G-11 banding and in situ hybridization. The results showed that CMGT can provide a wide variety of intermediate size of the transgenome from greater than 80,000kb to less than 2,000 kb. Some of transfectants are intergrated into mouse chromosome which can be detected by G-11 banding and in situ hybridization