Genetic analysis of the individual T-DNA genes of Agrobacterium tumefaciens; further evidence that two genes are involved in indole-3-acetic acid synthesis

Inzé, Dirk; Follin, A.; Lijsebettens, Mieke Van; Simoens, Chris; Genetello, C.; Montagu, Marc Van; Schell, Jeff

doi:10.1007/bf00383526

Cited by 229 publications

(78 citation statements)

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“…4. Novel pathways for plant hormone synthesis: T-DNA encoded genes for auxin and cytokinin biosynthesis responsible for tumor phenotype (Akiyoshi et al, 1984;Barry et al, 1984;Buchmann et al, 1985;Inze et al, 1984;Schro È der et al, 1984;Thomashow et al, 1984). 5.…”

Section: Nic Sites and Nicking Enzymes (Table 1 Item 8)mentioning

confidence: 99%

The transfer of DNA from Agrobacterium tumefaciens into plants: a feast of fundamental insights

et al. 2000

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This review is dedicated to Jeff Schell, one of the founders of modern`Agrobiology', the genetic and molecular dissection of crown gall disease. Together with notable scientists at the University of Gent, Belgium, Jeff spearheaded the discovery of the Ti-plasmid. The elusive`tumor inducing principle' was uncloaked and provided impetus for an incredibly fruitful subsequent 25 years of analyses. Scientists all over the world were caught up in unraveling the underlying mechanisms of Agrobacterium-mediated gene transfer to plants, and along the way uncovered a movable feast of fundamental insights. Below we summarize a sampling of Agrobacterium's most recently recognized accomplishments.

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Section: Nic Sites and Nicking Enzymes (Table 1 Item 8)mentioning

confidence: 99%

The transfer of DNA from Agrobacterium tumefaciens into plants: a feast of fundamental insights

et al. 2000

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“…The set of auxin genes is composed of two auxin genes ( aux1 and aux2) and an intergenic promoter sequence. aux1 and aux2 respectively encode tryptophan monooxygenase and indoleacetamide hydrolase, the enzymes needed for two-step synthesis of IAA [6], [7]. The cloning of auxin genes makes it possible to analyze the function of auxin genes in formation of leafy head.…”

Section: Introductionmentioning

confidence: 99%

Leafy head formation of the progenies of transgenic plants of Chinese cabbage with exogenous auxin genes

Xue

Sun

et al. 2000

Cell Res

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The experiment was performed to evaluate the progenies of plant lines transgenic for auxin synthesis genes derived from Ri T-DNA. Four lines of the transgenic plants were self-crossed and the foreign auxin genes in plants of T5 generation were confirmed by Southern hybridization. Two lines, D1232 and D1653, showed earlier folding of expanding leaves than untransformed line and therefore had early initiation of leafy head. Leaf cuttings derived from plant of transgenic line D1653 produced more adventitious roots than the control whereas the cuttings from folding leaves had much more roots than rosette leaves at folding stage, and the cuttings from head leaves had more roots than rosette leaves at heading stage. It is demonstrated that early folding of transgenic leaf may be caused by the relatively higher concentration of auxin. These plant lines with auxin transgenes can be used for the study of hormonal regulation in differentiation and development of plant organs and for the breeding of new variety with rapid growth trait.

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“…The iaaM gene encodes a mono-oxygenase which converts tryptophan to indole-3-acetamide [10,11]. Indole-3-acetamide is then hydrolyzed into indole-3-acetic acid by an aminohydrolase encoded by the iaaH gene [12,13]. Once the T-DNA is integrated into the plant genome, the expression of ipt, iaaM and iaaH results in elevated levels of these growth hormones culminating in the formation of the crown gall tumor [14,15].…”

Section: Introductionmentioning

confidence: 99%

A single amino acid substitution beyond the C2H2-zinc finger in Ros derepresses virulence and T-DNA genes in Agrobacterium tumefaciens

Archdeacon

2000

FEMS Microbiology Letters

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Ros is a chromosomally-encoded repressor containing a novel C2H2 zinc finger in Agrobacterium tumefaciens. Ros regulates the expression of six virulence genes and an oncogene on the Ti plasmid. Constitutive expression of these genes occurs in the spontaneous mutant 4011R derived from the octopine strain Ach-5, resulting in T-DNA processing in the absence of induction, and in the biosynthesis of cytokinin. Interestingly, the mutation in 4011R is an Arg to Cys conversion at amino acid residue 125 near the C-terminus well outside the zinc finger of Ros. Yet, Ros bearing this mutation is unable to bind to the Ros-box and is unable to complement other ros mutants. ß

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Genetic analysis of the individual T-DNA genes of Agrobacterium tumefaciens; further evidence that two genes are involved in indole-3-acetic acid synthesis

Cited by 229 publications

References 50 publications

The transfer of DNA from Agrobacterium tumefaciens into plants: a feast of fundamental insights

The transfer of DNA from Agrobacterium tumefaciens into plants: a feast of fundamental insights

Leafy head formation of the progenies of transgenic plants of Chinese cabbage with exogenous auxin genes

A single amino acid substitution beyond the C2H2-zinc finger in Ros derepresses virulence and T-DNA genes in Agrobacterium tumefaciens

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