Genetic Analysis of the Hypothalamic Neurotensin System

Garlow, Steven J.; Boone, Ericka; Kinkead, Becky; Nemeroff, Charles B.

doi:10.1038/sj.npp.1300870

Cited by 10 publications

(9 citation statements)

References 41 publications

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“…For CART, the B6 parent strain had a lower C t (i.e., higher transcript abundance) than the D2 strain. We have observed similar SDP amongst B6 and D2 strains in previous studies of hypothalamic peptide transcripts in our laboratory (Garlow et al, 2005(Garlow et al, , 2006. Comparison of the SDP for hypothalamic CART and corticotrophin-releasing factor (Garlow et al, 2005) transcription abundance expression reveals a significant correlation in strain mean transcript abundance between the targets (Pearson's r=0.46, p<0.0192).…”

Section: Resultssupporting

confidence: 54%

“…See Garlow et al, (2005Garlow et al, ( 2006 for complete RNA quantification methods. Amplification and detection primers for RT-PCR assays were from ABI "Assays-On-Demand" and included CART (ABI assay # Mm00489086; Accession # NM_013732.3), which generated an amplicon of 71 base pairs.…”

Section: Rna Quantificationmentioning

confidence: 99%

“…For a complete description of housing and colony conditions, please see Garlow et al, (2005Garlow et al, ( , 2006. Animals were sacrificed by cervical dislocation at PND 75-90, under low stress conditions.…”

Section: Animals and Tissue Preparationmentioning

confidence: 99%

“…Quantitative trait analysis, using panels of BxD recombinant inbred (RI) mice, continues to be one of the leading methods for the study of genes involved in complex neurobehavioral traits. The BxD series has been used to nominate and map QTL that contribute to a wide variety of complex traits including behavioral, pharmacological, neurochemical and neuroanatomical phenotypes (Erwin et al, 1997;Garlow et al, 2005Garlow et al, , 2006Jones et al, 1999Jones et al, , 2003Gora-Masalak et al, 1991;Belknap et al, 1992;Zhang and Gershenfeld 2003). The major goal of this study is to conduct quantitative trait analysis of CART expression in hypothalamus, by utilizing the BxD series of RI mice.…”

Section: Introductionmentioning

confidence: 99%

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Genetic regulation of hypothalamic cocaine and amphetamine-regulated transcript (CART) in BxD inbred mice

Boone

Hawks

et al. 2008

Brain Research

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Cocaine-Amphetamine Regulated Transcript (CART) peptides are implicated in a wide range of behaviors including in the reinforcing properties of psychostimulants, feeding and energy balance and stress and anxiety responses. We conducted a complex trait analysis to examine natural variation in the regulation of CART transcript abundance (CARTta) in the hypothalamus. CART transcript abundance was measured in total hypothalamic RNA from 26 BxD recombinant inbred (RI) mouse strains and in the C57BL/6 (B6) and DBA/2J (D2) progenitor strains. The strain distribution pattern for CARTta was continuous across the RI panel, which is consistent with this being a quantitative trait. Marker regression and interval mapping revealed significant quantitative trait loci (QTL) on mouse chromosome 4 (around 58.2cM) and chromosome 11 (between 20-36cM) that influence CARTta and account for 31% of the between strain variance in this phenotype. There are numerous candidate genes and QTL in these chromosomal regions that may indicate shared genetic regulation between CART expression and other neurobiological processes referable to known actions of this neuropeptide.

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Section: Resultssupporting

confidence: 54%

Section: Rna Quantificationmentioning

confidence: 99%

Section: Animals and Tissue Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic regulation of hypothalamic cocaine and amphetamine-regulated transcript (CART) in BxD inbred mice

Boone

Hawks

et al. 2008

Brain Research

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“…This confirmed that genes located on chromosome 1, which affect nerve system development (16), and on chromosome 19, which usually associate with genetic diseases (17), were mainly correlated with the development of NF1. The key cluster of genes that stimulate nerve growth on chromosomes 1 and 19 require identification in future studies.…”

supporting

confidence: 68%

Microarray-based identification of nerve growth-promoting genes in neurofibromatosis type I

Liu

Kang

et al. 2013

Molecular Medicine Reports

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Abstract. As a genetic disease, neurofibromatosis type 1 (NF1) is characterized by abnormalities in multiple tissues derived from the neural crest, including neoplasms in the ends of the limbs. The exact mechanism of nerve growth in NF1 is unclear. In the present study, the gene expression profile of nerves in healthy controls and NF1 patients with macrodactylia of the fingers or toes were analyzed in order to identify possible genes associated with nerve growth. The Whole Human Genome Microarray was selected to screen for different gene expression profiles, and the result was analyzed with Feature Extraction software. The target genes were verified at the transcriptional and translational levels by reverse transcription-polymerase chain reaction and western blotting, respectively. A common set of 28 genes were identified to be changed >5-fold in the NF1 patients compared with the healthy controls. Among them, the metastasis-associated genes, lymphocyte function-associated antigen 1, C-X-C chemokine receptor type 4 and intracellular adhesion molecule 1, and the inflammatory cytokines, interleukin (IL) 1β, IL-6 and IL-8, were downregulated significantly. Mainly genes that changed >10-fold were analyzed in this study, and HOXC8 demonstrated activity in promoting nerve growth. Through the analysis of the mRNA expression of the nerves in the NF1 patients, target molecules contributing to nerve growth during NF1 development were investigated, which aided in improving our understanding of this disease, and may provide a novel direction for nerve repair and regeneration.

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Evidence for genes on chromosome 2 contributing to alcohol dependence with conduct disorder and suicide attempts

Dick

Meyers

Alıev

et al. 2010

American J of Med Genetics Pt B

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Twin studies provide strong evidence that there is a shared genetic liability that predisposes to a number of different psychiatric outcomes related to behavioral disinhibition. Further, alcohol dependence comorbid with other disinhibitory disorders is particularly heritable. Chromosome 2p14–2q14.3 has been linked to multiple psychiatric conditions related to behavioral undercontrol. In the Collaborative Study on the Genetics of Alcoholism (COGA), we previously reported linkage to this region with alcohol dependence (AD), suicide attempts (SUI), and conduct disorder (CD). In this study, we follow-up on these previous reports of linkage by combining the phenotypes in analyses that jointly consider the presence of multiple conditions. Linkage analyses of the combined phenotype of AD with CD or SUI results in a maximum LOD score of 5.4 in this region. In addition to this primary linkage peak, independent samples have reported linkage to other alcohol-related phenotypes across chromosome 2. Accordingly, we followed-up these linkage signals by testing for association with SNPs across chromosome 2 in a case–control sample, in which a subset of the cases consisted of alcohol-dependent probands from the linkage sample. We find evidence of association with the combined AD with CD or SUI phenotype, with 23 genes surviving permutation testing. The number of associated genes across the chromosome may explain the persistent linkage findings reported on chromosome 2 across a number of independent studies of alcohol and disinhibitory phenotypes. Further, none of the genes were located directly under the primary COGA linkage peak, which has implications for association tests following-up linkage peaks.

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Genetic Analysis of the Hypothalamic Neurotensin System

Cited by 10 publications

References 41 publications

Genetic regulation of hypothalamic cocaine and amphetamine-regulated transcript (CART) in BxD inbred mice

Genetic regulation of hypothalamic cocaine and amphetamine-regulated transcript (CART) in BxD inbred mice

Microarray-based identification of nerve growth-promoting genes in neurofibromatosis type I

Evidence for genes on chromosome 2 contributing to alcohol dependence with conduct disorder and suicide attempts

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