Genetic analysis of the 15;17 chromosome translocation associated with acute promyelocytic leukemia.

Sheer, Denise; Hiorns, Lynne R.; Stanley, Karina; Goodfellow, Peter N.; Swallow, Dallas M.; Povey, S.; Heisterkamp, Nora; Groffen, John; Stephenson, John; Solomon, Ellen

doi:10.1073/pnas.80.16.5007

Cited by 43 publications

(16 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chromosomes from human lymphocytes, hybrids, and CMGT transfectants were all prepared as described (10). Additional I1To whom reprint requests should be addressed.…”

Section: Methodsmentioning

confidence: 99%

Construction of a genetic map of human chromosome 17 by use of chromosome-mediated gene transfer.

Xu¹,

Gorman²,

Rider³

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

We used somatic-cell hybrids, containing as their only human genetic contribution part or all of chromosome 17, as donors for chromosome-mediated gene transfer. A total of 54 independent transfectant clones were isolated and analyzed by use of probes or isoenzymes for >20 loci located on chromosome 17. By combining the data from this chromosomemediated gene transfer transfectant panel, conventional somatic-cell hybrids containing well-defined breaks on chromosome 17, and in situ hybridization, we propose the following order for these loci: pter- (i) although substantial lengths of DNA may be transferred intact (8, 9), interstitial deletions do frequently occur; (ii) multiple fragments of transfected chromatin can be found in the same clone; and (iii) there is also selection for centromeric sequences (8,9).In addition, during the course of this study we obtained some transfectants that contained only the small regions of chromosome 17 particularly relevant to the study of APL and von Recklinghausen neurofibromatosis. MATERIALS AND METHODSCulture Conditions. The cells were cultured either in RPMI 1640 or in Dulbecco's minimal essential medium supplemented with 10% fetal bovine serum. PCTBA1.8, PJT2/A1, and all CMGT transfectants, PLT, KLT, and TLT were maintained in HAT medium (100 ,uM hypoxanthine/10 ,M methotrexate/10 ,M thymidine), which selects for thymidine kinase. The hybrids GPT17.3.2K41 and TRID62 were maintained in MX medium [mycophenolic acid (25 ,ug/ml)/xanthine (250 ,g/ml)]. Back selection of CMGT transfectants with 5-bromo-2-deoxyuridine was done by the addition of 5-bromo-2-deoxyuridine (50 ,g/ml) to the medium, thus selecting for those CMGT transfectants that have lost the TKI locus. This medium was replenished every 3 days for 2-3 weeks. Resulting colonies were maintained in RPMI 1640/ 10% fetal bovine serum and were denoted by the letter B after the name of the original transfectant.Cytogenetic Analysis. Chromosomes from human lymphocytes, hybrids, and CMGT transfectants were all prepared as described (10). Additional

show abstract

“…Chromosomes from human lymphocytes, hybrids, and CMGT transfectants were all prepared as described (10). Additional I1To whom reprint requests should be addressed.…”

Section: Methodsmentioning

confidence: 99%

Construction of a genetic map of human chromosome 17 by use of chromosome-mediated gene transfer.

Xu¹,

Gorman²,

Rider³

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Associated with the polymorphic glycoproteins of the HLA-A, -B, and -C antigens is a nonglycosylated 12,000-dalton invariant chain, 82-microglobulin, that has been assigned to human chromosome 15 (20,21). The class II genes, or the immune response genes, consist of heterodimers composed of a 34,000-dalton (HLA-DR a) glycoprotein for which no electrophoretic polymorphisms have been demonstrated and a polymorphic 29,000-dalton (HLA-DR (3) glycopeptide (21)(22)(23)(24)(25). Polymorphism of the HLA-DR , gene has permitted a regional assignment of this locus to chromosome 6 proximal to 6p22.4 by a family study (15).…”

Section: Introductionmentioning

confidence: 99%

“…Genetic mapping by somatic cell hybridization (3)(4)(5)(6)(7) and by the segregation of unique chromosomal rearrangements involving the MHC in families (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) has permitted localization of the serologically polymorphic 45,000-dalton glycoproteins of the class I genes (HLA-A, -B, and -C) to the short arm of human chromosome 6, with the shortest region of overlap being the 6p21 band (7,17). Associated with the polymorphic glycoproteins of the HLA-A, -B, and -C antigens is a nonglycosylated 12,000-dalton invariant chain, 82-microglobulin, that has been assigned to human chromosome 15 (20,21). The class II genes, or the immune response genes, consist of heterodimers composed of a 34,000-dalton (HLA-DR a) glycoprotein for which no electrophoretic polymorphisms have been demonstrated and a polymorphic 29,000-dalton (HLA-DR (3) glycopeptide (21)(22)(23)(24)(25).…”

mentioning

confidence: 99%

Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization.

Kirsch¹,

Nance²,

Evans³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

We have determined the localization and orientation of two genetic probes within the human major histocompatibility complex by chromosomal in situ hybridization.Our data indicate that a cloned genomic probe cross-hybridizing to HLA-A, -B, and -C heavy chain loci is homologous to sequences located on chromosome 6 at band p21.3 while a subclone of the genomic HLA-DR a-chain gene corresponding to the nonpolymorphic p34 protein is homologous to sequences in band 6p21.1. Our data suggest that this technique may permit the estimation of map distances between linked gene loci, assuming a uniform frequency of map units in the human genome. The relative positions of these genes was confirmed in a mother and son carrying a chromosome rearrangement involving 6p and 14p in which the sequences hybridizing to a DR a-chain genomic clone were found at the distal end of the 6p-chromosome [der(6)] while the sequences hybridizing to the HLA-A, -B, -C a-chain probe were found in the 14p+ chromosome [der(14)].The major histocompatibility complex (MHC) in humans is a cluster of at least three gene families encoding cell surface glycoproteins-i.e., genes for class I (HLA-A, -B, and -C) and class II (HLA-DR) antigens in addition to class III genes (complement factors)-and spans 2-3 x 103 kilobase pairs (kb) of DNA (1, 2). Genetic mapping by somatic cell hybridization (3-7) and by the segregation of unique chromosomal rearrangements involving the MHC in families (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) has permitted localization of the serologically polymorphic 45,000-dalton glycoproteins of the class I genes (HLA-A, -B, and -C) to the short arm of human chromosome 6, with the shortest region of overlap being the 6p21 band (7,17). Associated with the polymorphic glycoproteins of the HLA-A, -B, and -C antigens is a nonglycosylated 12,000-dalton invariant chain, 82-microglobulin, that has been assigned to human chromosome 15 (20,21). The class II genes, or the immune response genes, consist of heterodimers composed of a 34,000-dalton (HLA-DR a) glycoprotein for which no electrophoretic polymorphisms have been demonstrated and a polymorphic 29,000-dalton (HLA-DR (3) glycopeptide (21)(22)(23)(24)(25). Polymorphism of the HLA-DR , gene has permitted a regional assignment of this locus to chromosome 6 proximal to 6p22.4 by a family study (15).Recently, the HLA-DR a-chain locus has been mapped by using somatic cell hybrids (26) and, furthermore, this assignment has been refined to the region 6p2105-6p23 (27) by study of genomic blotting patterns in cell lines containing individual chromosomal deletions of the short arm of chromosome 6. Data on recombination within the HLA complex and with closely linked markers such as glyoxylase 1 (GLO 1) have permitted inferences to be drawn about the order and map distances between marker loci on the proximal portion of the short arm of chromosome 6, as follows: GLO 1-4 centimorgans (cM)-HLA-DR-0.3 cM-BF, C4B, C4A, C2-0.7 cM-HLA-B-0.1 cM-HLA-C-0.7 cM-HLA-A (16-18).Improvements in chromosomal i...

show abstract

“…Q-and G-banding has resulted in general agreement over the siting of the breakpoints at 15q22, possibly 15q2200, and 17q12-21 (Fourth International Workshop on Chromosomes in Leukemia, 1984;Hagemeijer et al, 1982;Larson et al, 1984). Analysis of interspecific somatic cell hybrids derived from one APL patient (JD) has confirmed the localisation of the translocation breakpoints to 15q22 and 17ql2-21 (Sheer et al, 1983).…”

Section: Methodsmentioning

confidence: 85%

“…The chromosome breakpoints in one patient (J.D.) were localised to 15q22 and 17ql2-21 by genetic analysis of an interspecific somatic cell hybrid containing the 15q+ translocation chromosome (Sheer et al, 1983). No other clonal chromosome abnormalities were found, other than trisomy 10 which was present in 50% of metaphases analysed in one patient.…”

Section: Methodsmentioning

confidence: 94%

Incidence of the 15q+;17q- chromosome translocation in acute promyelocytic leukaemia (APL)

Sheer¹,

Lister²,

Amess³

et al. 1985

Br J Cancer

Self Cite

View full text Add to dashboard Cite

Summary Cytogenetic analysis was carried out on peripheral blood cultures from seven patients with acute promyelocytic leukaemia . A reciprocal 15;17 chromosome translocation, t(lSq +; 17q -), was found in all cases, and the breakpoints estimated to be 15q22 and 17ql2-21. In addition to the t(15q+;17q-), trisomy 10 was found in 50% of cells analysed in one case. These results suggest that the 15;17 chromosome translocation may be observed in most cases of APL where the leukaemic cells are cultured before cytogenetic analysis is performed. The use of conditioned media in the culture of leukaemic cells is also described.

show abstract

Genetic analysis of the 15;17 chromosome translocation associated with acute promyelocytic leukemia.

Cited by 43 publications

References 47 publications

Construction of a genetic map of human chromosome 17 by use of chromosome-mediated gene transfer.

Construction of a genetic map of human chromosome 17 by use of chromosome-mediated gene transfer.

Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization.

Incidence of the 15q+;17q- chromosome translocation in acute promyelocytic leukaemia (APL)

Contact Info

Product

Resources

About