Genetic analysis of testis weight and fertility in an interspecies hybrid congenic strain for Chromosome X

Rw, Elliott; Miller, Darla R.; Rs, Pearsall; Hohman, Colleen; Zhang, Y; Poslinski, Diane; Da, Tabaczynski; Vm, Chapman

doi:10.1007/s003350010234

Cited by 32 publications

(18 citation statements)

References 27 publications

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“…To avoid the deleterious effect of these genes in further generations, the production of IRCSs was started from fertile backcross males. Of 157 N 2 males, 18 (11%, approximately one-eighth) proved to be fertile, consistent with the previous report of three genetically independent hybrid sterility genes ( Guénet et al 1990;Pilder 1997;Elliott et al 2001). …”

Section: Establishment Of Ircsssupporting

confidence: 90%

Interspecific Recombinant Congenic Strains Between C57BL/6 and Mice of the Mus spretus Species: A Powerful Tool to Dissect Genetic Control of Complex Traits

Burgio

Szatanik

Guénet³

et al. 2007

Genetics

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Complex traits are under the genetic control of multiple genes, often with weak effects and strong epistatic interactions. We developed two new collections of mouse strains to improve genetic dissection of complex traits. They are derived from several backcrosses of the Mus spretus SEG/Pas or STF/Pas strains on the C57BL/6J background. Each of the 55 interspecific recombinant congenic strains (IRCSs) carries up to eight SEG/Pas chromosomal segments with an average size of 11.7 Mb, totalizing 1.37% of the genome. The complete series covers 39.7% of the SEG/Pas genome. As a complementary resource, six partial or complete interspecific consomic strains were developed and increased genome coverage to 45.6%. To evaluate the usefulness of these strains for QTL mapping, 16 IRCSs were compared with C57BL/6J for seven hematological parameters. Strain 66H, which carries three SEG/Pas chromosomal segments, had lower red blood cell volume and higher platelet count than C57BL/6J. Each chromosomal segment was isolated in a congenic strain to evaluate individual effects. Congenic strains were combined to assess epistasis. Our data show that both traits were controlled by several genes with complex epistatic interactions. IRCSs are therefore useful to unravel QTL with small effects and gene-by-gene interactions.

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Section: Establishment Of Ircsssupporting

confidence: 90%

Interspecific Recombinant Congenic Strains Between C57BL/6 and Mice of the Mus spretus Species: A Powerful Tool to Dissect Genetic Control of Complex Traits

Burgio

Szatanik

Guénet³

et al. 2007

Genetics

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“…Molecular markers on the X chromosome show reduced introgression (relative to the autosomal loci surveyed) across the European hybrid zone between M. domesticus and M. musculus (Tucker et al 1992;Dod et al 1993;Munclinger et al 2002), and the X chromosome has been associated with hybrid male sterility in crosses involving lab strains (Guenet et al 1990;Elliott et al 2001;Storchova et al 2004). In particular, an X-linked QTL for hybrid male sterility (Hstx1) was recently localized to an interval between 64.0 and 70.1 Mb by introgressing pieces of the wild M. musculus X chromosome on to the autosomal background of C57BL/6 (Storchova et al 2004).…”

Section: Reproductive Isolation In Musmentioning

confidence: 99%

Signatures of Reproductive Isolation in Patterns of Single Nucleotide Diversity Across Inbred Strains of Mice

Payseur

Hoekstra

2005

Genetics

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Reproductive isolation is often caused by the disruption of genic interactions that evolve in geographically separate populations. Identifying the genomic regions and genes involved in these interactions, known as ''Dobzhansky-Muller incompatibilities,'' can be challenging but is facilitated by the wealth of genetic markers now available in model systems. In recent years, the complete genome sequence and thousands of single nucleotide polymorphisms (SNPs) from laboratory mice, which are largely genetic hybrids between Mus musculus and M. domesticus, have become available. Here, we use these resources to locate genomic regions that may underlie reproductive isolation between these two species. Using genotypes from 332 SNPs that differ between wild-derived strains of M. musculus and M. domesticus, we identified several physically unlinked SNP pairs that show exceptional gametic disequilibrium across the lab strains. Conspecific alleles were associated in a disproportionate number of these cases, consistent with the action of natural selection against hybrid gene combinations. As predicted by the Dobzhansky-Muller model, this bias was differentially attributable to locus pairs for which one hybrid genotype was missing. We assembled a list of potential Dobzhansky-Muller incompatibilities from locus pairs that showed extreme associations (only three gametic types) among conspecific alleles. Two SNPs in this list map near known hybrid sterility loci on chromosome 17 and the X chromosome, allowing us to nominate partners for disrupted interactions involving these genomic regions for the first time. Together, these results indicate that patterns produced by speciation between M. musculus and M. domesticus are visible in the genomes of lab strains of mice, underscoring the potential of these genetic model organisms for addressing general questions in evolutionary biology.

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“…These animals include Drosophila (Joly et al 1997), stickleback fish Pungitius (Takahashi et al 2005), caviomorph rodent Thrichomys (Borodin et al 2006), house musk shrew Suncus (Borodin et al 1998), wallaby Petrogale (Close et al 1996), and genus Mus (Forejt and Iványi 1974;Matsuda et al 1992;Hale et al 1993;Yoshiki et al 1993;Kaku et al 1995;Gregorová and Forejt 2000;Elliott et al 2001Elliott et al , 2004Good et al 2008). Although reproductive isolation by spermatogenic impairment is a well-known phenomenon, its underlying genetic mechanism and molecular basis have remained elusive.…”

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confidence: 99%

Reproductive Isolation in Hybrid Mice Due to Spermatogenesis Defects at Three Meiotic Stages

et al. 2010

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Early in the process of speciation, reproductive failures occur in hybrid animals between genetically diverged populations. The sterile hybrid animals are often males in mammals and they exhibit spermatogenic disruptions, resulting in decreased number and/or malformation of mature sperms. Despite the generality of this phenomenon, comparative study of phenotypes in hybrid males from various crosses has not been done, and therefore the comprehensive genetic basis of the disruption is still elusive. In this study, we characterized the spermatogenic phenotype especially during meiosis in four different cases of reproductive isolation: B6-ChrX MSM , PGN-ChrX MSM , (B6 3 Mus musculus musculus-NJL/Ms) F 1 , and (B6 3 Mus spretus) F 1 . The first two are consomic strains, both bearing the X chromosome of M. m. molossinus; in B6-ChrX MSM , the genetic background is the laboratory strain C57BL/6J (predominantly M. m. domesticus), while in PGN-ChrX MSM the background is the PGN2/Ms strain purely derived from wild M. m. domesticus. The last two cases are F 1 hybrids between mouse subspecies or species. Each of the hybrid males exhibited cell-cycle arrest and/or apoptosis at either one or two of three distinct meiotic stages: premeiotic stage, zygotene-to-pachytene stage of prophase I, and metaphase I. This study shows that the sterility in hybrid males is caused by spermatogenic disruptions at multiple stages, suggesting that the responsible genes function in different cellular processes. Furthermore, the stages with disruptions are not correlated with the genetic distance between the respective parental strains.

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Genetic analysis of testis weight and fertility in an interspecies hybrid congenic strain for Chromosome X

Cited by 32 publications

References 27 publications

Interspecific Recombinant Congenic Strains Between C57BL/6 and Mice of the Mus spretus Species: A Powerful Tool to Dissect Genetic Control of Complex Traits

Interspecific Recombinant Congenic Strains Between C57BL/6 and Mice of the Mus spretus Species: A Powerful Tool to Dissect Genetic Control of Complex Traits

Signatures of Reproductive Isolation in Patterns of Single Nucleotide Diversity Across Inbred Strains of Mice

Reproductive Isolation in Hybrid Mice Due to Spermatogenesis Defects at Three Meiotic Stages

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