Genetic analysis of stripe rust resistance in a set of European winter wheat genotypes

Identification of a novel stripe rust resistance gene from the European winter wheat cultivar ‘Acienda’: A step towards rust proofing wheat cultivation

“…In this study we report the phenotypic and genetic characterisation of a stripe rust resistance locus Yraci from the European winter wheat cultivar ‘Acienda’. Analysis of segregation patterns of resistance in backcross populations developed from ‘Acienda’ and the Indian spring wheat ‘HD 2967’ support monogenic inheritance of the locus, confirming the previous findings in [ 12 ]. Genotyping the backcross population with the high-density 35K Affymetrix Wheat Breeders SNP array produced 2,359 informative markers for mapping.…”

“…In India, Acienda showed strong resistance to all prevalent stripe rust races and pathotypes in field screening experiments. Further, it did not show presence of other effective stripe rust resistance genes currently in use in India when screened with available linked molecular markers [ 12 ]. Via backcross introgression into an agronomically elite Indian spring wheat background we mapped the novel Yraci locus to chromosome 1A and created advanced germplasm carrying the locus for use in breeding.…”

“…The European winter wheat cultivar ‘Acienda’ was identified as having all stage stripe rust resistance in field trials at Punjab Agricultural University (PAU), Ludhiana, India (30° 54’ N latitude, 75° 48’ E longitude, and 247m above m s l) over five seasons (2011–2016) [ 12 ]. The resistant donor parent ‘Acienda’ was selected from the 376-line European winter wheat Triticeae Genome association mapping panel [ 20 ].…”

“…Plant material was inoculated with the mixture of major stripe rust pathotypes Pst 78S84, Pst 46S119, 110S119 and 238S119 [ 17 ]. Primary inoculations and disease pressure were built up as per the standard protocol [ 12 ]. Infector rows of the susceptible cultivars ‘PBW 343’ and ‘HD 2967’ were planted at the borders of each population to ensure disease pressure.…”

“…A single dominant stripe rust resistance source in the European bread wheat cultivar ‘Acienda’ was reported in our previous report [ 12 ]. In the present study, we report molecular mapping of this all-stage stripe rust resistance gene using high throughput SNP genotyping in backcross populations.…”