Genetic analysis of starch paste viscosity parameters in glutinous rice (Oryza sativa L.)

Abstract

Starch paste viscosity plays an important role in estimating the cooking, eating, and processing quality of rice. The inheritance of starch paste viscosity in glutinous rice remains undefined. In the present study, 118 glutinous rice accessions were collected, and the genotypes of 17 starch synthesis-related genes (SSRG) were analyzed by using 43 gene-specific molecular markers. Association analysis indicated that 10 of 17 SSRGs were involved in controlling the rapid visco analyzer (RVA) profile parameters. Among these, the PUL gene was identified to play an important role in control of peak viscosity (PKV), hot paste viscosity (HPV), cool paste viscosity (CPV), breakdown viscosity (BDV), peak time (PeT), and paste temperature (PaT) in glutinous rice. Other SSRGs involved only a few RVA profile parameters. Furthermore, interactions between SSRGs were found being responsible for PeT, PaT, and BDV. Some of the RVA parameters, including PKV, HPV, CPV, CSV, and PaT, were mainly governed by single SSRG, whereas other parameters, such as BDV, SBV, and PeT, were controlled by a few SSRGs, functioning cooperatively. Further, three near-isogenic lines (NIL) of a japonica glutinous cv. Suyunuo as genetic background, with PUL, SSIII-1, and SSIII-2 alleles replaced with those of indica cv. Guichao 2, were employed to verify the genetic effects of the various genes, and the results were consistent with those obtained from the association analysis. These findings indicated that starch paste viscosity in glutinous rice had a complex genetic system, and the PUL gene played an important role in determining the RVA profile parameters in glutinous rice. These results provide important information for potentially improving the quality of glutinous rice.

Analysis of minor quantitative trait loci for eating and cooking quality traits in rice using a recombinant inbred line population derived from two indica cultivars with similar amylose content

“…Pullulanase plays a dominant role in control of peak viscosity (PKV), hot paste viscosity (HPV), cool paste viscosity (CPV), breakdown viscosity (BDV), peak time, and pasting temperature in glutinous rice. Yan et al (2011) found that 10 of 17 starch-related genes had effects on RVA profile parameters, whereas nine other-related starch genes had minor effects on only some RVA profile parameters. However, RVA parameters such as starch paste viscosity and other starch quality traits are probably controlled by a complex genetic system involving many starch-related genes ).…”

Effects of allelic variations in starch synthesis-related genes on grain quality traits of Korean nonglutinous rice varieties under different temperature conditions

“…Tanaka et al (2009) reported that PKV and BDV increased while SBV decreased significantly under high temperature in three Japonic a rice varieties. Zhong et al (2005) showed that CPV and SBV decreased significantly under high temperature in four Indica rice varieties. In this study, the average BDV of the 187 varieties increased significantly as the temperature rose while the other RVA characteristics and ADV did not change significantly in response to higher temperature.…”

“…Lee et al (2009) sequenced complete or partial genic regions of eight SSRGs from 27 rice varieties with diverse genetic backgrounds and developed 25 cleaved amplified polymorphic sequence (CAPs) or derived CAPs markers, among which SNPs in GBSSI and AGPase-S were strongly associated with AC. Yan et al (2011) developed 43 sequence tagged site (STS) and CAPs markers for genotyping glutinous rice germplasms by sequencing 17 SSRGs from 13 representative varieties and studied the roles of SSRGs in controlling the RVA characteristics of 118 glutinous rice varieties. Through massive parallel sequencing, Kharabian-Masouleh et al (2011) found 501 SNPs and 113 insertion/deletion (InDels) markers in 17 SSRGs from 233 Australian rice breeding lines.…”

A Novel Mutation of OsPPDKB, Encoding Pyruvate Orthophosphate Dikinase, Affects Metabolism and Structure of Starch in the Rice Endosperm

“…The low amylose content of M14 led to different RVA profiles, including the PV, HV, and FV (Figure 3E; Supplemental Table S1). GBSSI , SSIIa and PUL together with BEI and BEIIb affect the starch pasting properties in rice [30,31,32]. Indeed, the expression of these starch synthesis genes significantly reduced in M14 (Figure 7) and, as a result, the pasting properties of M14 starch also changed.…”