Genetic analysis of sperm function in fertilization

Olds‐Clarke, Patricia

doi:10.1002/mrd.1120200214

Cited by 27 publications

(10 citation statements)

References 173 publications

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“…However, intraspeci®c studies are rare. Within species, the most sensitive test of this prediction is to compare the fertilization success of males derived from different populations, under conditions of sperm competition (Olds-Clarke, 1988;Price, 1997). The ubiquity of conspeci®c sperm precedence (Price, 1997) supports this notion at a higher taxonomic level, whilst the intraspeci®c studies on C. maculatus (pers.…”

Section: Discussionmentioning

confidence: 87%

“…The oocytes in most mammalian species are surrounded by at least two investing layers, the zona pellucida and the cumulus oophorus, with sperm±egg interactions being highly complex, presenting many opportunities for sperm selection. Intraspeci®c sperm selection can be implied by the study of Wabik-Sliz (1985), who found that the susceptibility of oocyte investments (both cumulus oophorus and zona pellucida) to enzymatic digestion differed among various strains of mice, whilst the sperm of different strains of mice have been shown to vary in relation to glycosidase activity (an acrosome enzyme) and level of surface galactosyltransferase (thought to be involved with sperm±zona binding) (Olds-Clarke, 1988).…”

Section: Mechanisms and Sites Of Sperm Selectionmentioning

confidence: 99%

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Postcopulatory, prezygotic reproductive isolation

Eady

2001

Journal of Zoology

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Prezygotic reproductive isolation has traditionally been studied from a precopulatory perspective even though postcopulatory events have been known to influence success at fertilization. Postcopulatory, prezygotic reproductive isolation (gametic isolation) has received relatively little attention mainly because the focus of research has been on the events that occur either earlier (pre-mating) or later (postzygotic) in the chain of events that lead to successful reproduction. However, recent evidence from an array of taxa from sea urchins to beetles reveals that postejaculatory, prezygotic events may be an important factor in reproductive isolation. Such a mechanism may be the driving force behind speciation in some taxa, such as free-spawning marine invertebrates, whilst it may complement pre-mating and postzygotic mechanisms in others. In this paper we draw an analogy between sperm–egg interactions and classical sexual selection theory and argue that gametic incompatibility may arise through cryptic female choice

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Section: Discussionmentioning

confidence: 87%

Section: Mechanisms and Sites Of Sperm Selectionmentioning

confidence: 99%

Postcopulatory, prezygotic reproductive isolation

Eady

2001

Journal of Zoology

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“…For example, competition between sperm from different mouse strains incorporated into male chimeras was only apparent when mating occurred after ovulation (Buehr and McLaren, 1984). Conversely, prolonged motility or survival might confer a selective advantage if ovulation occurred later and sperm were already waiting in the oviduct (see Braden, 1958;McGrath and Hillman, 1980;Olds-Clarke, 1988, 1997 for discussion). The IVF experiments provide direct evidence that sperm function is compromised in HT mice and rule out the possibility that the effect is mediated solely by differential rates of sperm transport to the oviduct.…”

Section: Discussionmentioning

confidence: 96%

“…Since glycolysis is important for ATP generation and fertilization in mouse sperm (Fraser and Quinn, 1981), this would compromise sperm function. Other unknown genetic differences may also result in sperm selection in mixed inseminations as described elsewhere (Edwards, 1955;Overstreet and Adams, 1971;Sharma and Hays, 1975;Buehr and McLaren, 1984;Olds-Clarke, 1988).…”

Section: Discussionmentioning

confidence: 98%

Failure of founder transgenic male mice to transmit an attenuated HSV thymidine kinase transgene results from mosaicism and sperm competition

et al. 2000

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“…There is substantial genotypic variability within the AE24 outbred line, which could lead to the independent assortment of secondary genetic elements affecting fertility. Expression of the sterile phenotype in male mice can be strictly dependent on genetic background, including sterility associated with the t complex, the hybrid sterility genes 1 and 2 , and specific autosomal chromosomal translocations (Forejt and Ivanyi 1975;Forejt 1976;Olds-Clarke and McCabe 1982;deBoer 1986;Olds-Clarke 1988).…”

Section: Incomplete Penetrance Of the Sterile Phenotypementioning

confidence: 99%

Inactivation of a sperm motility gene by insertion of an epidermal growth factor receptor transgene whose product is overexpressed and compartmentalized during spermatogenesis.

Merlino¹,

Stahle²,

Jhappan³

et al. 1991

Genes Dev.

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Transgenic mice were generated with a human epidermal growth factor (EGF) receptor cDNA driven by the chicken 13-actin gene promoter. One line (AE24) that exhibited a unique expression pattern in which dramatically elevated levels of EGF receptor RNA were found only in the testis was established, suggesting that the 13-actin promoter was being influenced by an adjacent testis-specific enhancer. EGF receptor RNA was detected in primary spermatocytes, whereas the synthesis of receptor protein was restricted to elongate spermatids, indicating that transgene expression was under translational control. At spermiation, the EGF receptor was sequestered in residual bodies and excluded from mature sperm by a compartmentalization mechanism. About half of AE24 homozygous males were sterile because of sperm paralysis, whereas heterozygous males and females of either genotype were completely fertile. Electron microscopic analysis of sperm flagella from sterile AE24 homozygotes revealed an aberrant axonemal structure in which outer doublet microtubules were missing from the middle piece, resembling changes observed in the sperm of some infertile humans. Flagellar axonemal disassembly was observed in the vas deferens and epididymis but not in the testis, suggesting that outer doublets were assembled in a grossly normal manner but possessed a latent instability. These results demonstrate that in the AE24 mouse line the EGF receptor transgene was integrated into and inactivated an endogenous autosomal gene, causing sperm flagellar axonemal disruption and male sterility.

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Genetic analysis of sperm function in fertilization

Cited by 27 publications

References 173 publications

Postcopulatory, prezygotic reproductive isolation

Postcopulatory, prezygotic reproductive isolation

Failure of founder transgenic male mice to transmit an attenuated HSV thymidine kinase transgene results from mosaicism and sperm competition

Inactivation of a sperm motility gene by insertion of an epidermal growth factor receptor transgene whose product is overexpressed and compartmentalized during spermatogenesis.

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