Genetic analysis of pod and seed resistance to pea weevil in a Pisum sativum×P. fulvum interspecific cross

Abstract

Interspecific populations derived from crossing cultivated field pea, Pisum sativum, with the wild pea relative, Pisum fulvum, were scored for pod and seed injury caused by the pea weevil, Bruchus pisorum. Pod resistance was quantitatively inherited in the F2 population, with evidence of transgressive segregation. Heritability of pod resistance between F2 and F3 generations was very low, suggesting that this trait would be difficult to transfer in a breeding program. Seed resistance was determined for the F2 population by testing F3 seed tissues of individual F2 plants and pooling data from seed reaction for each F2 plant (inferred F2 genotype). Segregation for seed resistance in the F2 population of the cross Pennant/ATC113 showed a trigenic mode of inheritance, with additive effects and dominant epistasis towards susceptibility. Seed resistance was conserved over consecutive generations (F2 to F5) and successfully transferred to a new population by backcross introgression. Seed resistance in the backcross introgressed population segregated in a 63 : 1 ratio, supporting the three-gene inheritance model. It is proposed that complete resistance to pea weevil is controlled by three major recessive alleles assigned pwr1, pwr2, and pwr3, and complete susceptibility by three major dominant alleles assigned PWR1, PWR2, and PWR3. It is recommended that large populations (>300 F2 plants) would be required to effectively transfer these recessive alleles to current field pea cultivars through hybridisation and repeated backcrossing.

Legume Crops Phylogeny and Genetic Diversity for Science and Breeding

“…Many pea germplasm screens have been conducted for biotic and abiotic stresses, agronomic traits and seed quality (e.g., nutrition) but the studies are small (less than 20 accessions), the data is unavailable, published in difficult-to-access sources or unpublished. The most attention has been given to P. fulvum, as a donor of bruchid resistance and source of novel powdery mildew resistance (Clement et al, 2002(Clement et al, , 2009Fondevilla et al, , 2008Byrne et al, 2008). Incomplete levels of resistance to powdery mildew, rust (Uromyces pisi (Pers.)…”

“…Durieu and Ochatt (2000) have tested protoplast fusion and regeneration of calli between Pisum sativum and Lathyrus sativus, and although heterokaryons were detected and up to 6 cell divisions were observed, no further growth or plant regeneration could be achieved. Pisum fulvum was used to introduce resistance to powdery mildew , bruchid pests (Clement et al, 2002(Clement et al, , 2009Byrne et al, 2008) and Orobanche crenata , while primitive landraces were used in order to incorporate virus and Fusarium resistances (Providenti, 1990;McPhee et al, 1999). Wild accessions of P. sativum subsp.…”

Importance of Spatial and Spectral Data Reduction in the Detection of Internal Defects in Food Products

“…Five commercial field pea cultivars, which are grown mainly for human consumption (Pennant, Dunwa, Helena, Kaspa, and Yarrum) and seven pea weevil-resistant field pea backcross lines (BC 1 F 6 ) 34,35 were included as the training data in this study; their average reflection profiles are presented in Fig. 1a.…”

Breeding Food Legumes for Resistance to Storage Insect Pests: Potential and Limitations

“…When available, the information often suggests limited success. For instance, genetic analysis of pod and seed resistance to pea weevil (B. pisorum) in an inter-specific cross between Pisum sativum × P. fulvum showed low heritability values implying that only a limited success is expected from the breeding program [68].…”