Genetic analysis of phytic acid content in pearl millet

Satija, D. R.; Thukral, Swati

doi:10.1007/bf00252299

Cited by 9 publications

(11 citation statements)

References 9 publications

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“…Development of cultivars with low phytin content is another way to increase bioavailability. The reduction of phytin content in seeds is one of the major breeding objectives in several crops, including wheat (Batten, 1986), field beans (Griffiths and Thomas, 1981), soybean (Raboy et al, 1984), and pearl millet (Satija and Thukral, 1985). One problem, however, is that cultivars with a low phytin content often have low phosphorus and protein contents as well (Raboy et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Temporal and Spatial Patterns of Accumulation of the Transcript of Myo-Inositol-1-Phosphate Synthase and Phytin-Containing Particles during Seed Development in Rice1

et al. 1999

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Myo-inositol-1-phosphate (I[1]P) synthase (EC 5.5.1.4) catalyzes the reaction from glucose 6-phosphate to I(1)P, the first step of myo-inositol biosynthesis. Among the metabolites of I(1)P is inositol hexakisphosphate, which forms a mixed salt called phytin or phytate, a storage form of phosphate and cations in seeds. We have isolated a rice (Oryza sativa L.) cDNA clone, pRINO1, that is highly homologous to the I(1)P synthase from yeast and plants. Northern analysis of total RNA showed that the transcript accumulated to high levels in embryos but was undetectable in shoots, roots, and flowers. In situ hybridization of developing seeds showed that the transcript first appeared in the apical region of globular-stage embryos 2 d after anthesis (DAA). Strong signals were detected in the scutellum and aleurone layer after 4 DAA. The level of the transcript in these cells increased until 7 DAA, after which time it gradually decreased. Phytin-containing particles called globoids appeared 4 DAA in the scutellum and aleurone layer, coinciding with the localization of the RINO1 transcript. The temporal and spatial patterns of accumulation of the RINO1 transcript and globoids suggest that I(1)P synthase directs phytin biosynthesis in rice seeds.Myo-inositol and I(1)P are essential for the survival of eukaryotic cells, because various metabolic routes diverge from these two compounds. A number of possible roles for myo-inositol and I(1)P metabolites in plant development have been demonstrated (for review, see Bohnert et al., 1995). I(1)P is utilized for the synthesis of plasma membrane phosphoinositides, which are involved in signal transduction as sources of second messengers (Gross and Boss, 1993). Myo-inositol combined with Gal is incorporated into the raffinose family of the vegetative storage form of carbohydrates (Kandler and Hopf, 1982). Oxidized inositols serve as noncellulosic cell wall components (Loewus and Loewus, 1983), and methylated inositols were shown to be involved in osmoprotection in a halophytic plant (Ishitani et al., 1996). In addition, I(1)P is further phosphorylated to inositol hexakisphosphoric acid, or phytic acid, which strongly binds metallic cations such as K, Mg, Mn, Ca, Fe, and Zn via ionic associations with negatively charged phosphates (Maga, 1982;Lott et al., 1995) to form a mixed salt called phytin or phytate (Ashton, 1976). The synthesis of phytin as a storage form of phosphate and cations is a metabolic pathway unique to plants (Loewus and Dickinson, 1982).Phytin is mainly stored in protein bodies in seeds as spherical inclusions called globoids (Lott et al., 1995). Cereal grains and oil seeds are particularly rich sources of phytin, and the phosphorus of phytin represents 80% to 90% of total phosphorus in these seeds (Maga, 1982). In rice (Oryza sativa) seeds phytin accumulates in the aleurone and scutellum cells but not in the starchy endosperm cells (Tanaka et al., 1973;Ogawa et al., 1977). During germination phytin is digested by an acid phosphatase called phytase (Laboure et al....

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Section: Discussionmentioning

confidence: 99%

Temporal and Spatial Patterns of Accumulation of the Transcript of Myo-Inositol-1-Phosphate Synthase and Phytin-Containing Particles during Seed Development in Rice1

et al. 1999

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“…2), which is in accordance with the results of Ahmad et al (2009) in tomato, Hemanthkumar et al (2008) in gladiolus, Hussein and Misiha (1978) in petunia and Lou et al (2010) in zinnia. Romanus et al (2008) and also Satija and Thukral (1985) have provided evidence for the greater importance of additive gene action in hybrid breeding outcomes. Reciprocal effects mean squares were significant and highly significant for all the measured traits, showing that inheritance of these traits governs not only nuclear but also plasma-genes (Tab.…”

Section: Resultsmentioning

confidence: 99%

Estimation of Heterosis and Combining Ability in Petunia (Petunia hybrida Hort.)

et al. 2012

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Combining ability and heterosis were estimated for ornamental and vegetative traits of four petunia (Petunia hybrida Hort.) inbred lines viz. L5 (P1), L8 (P2), L11 (P3) and L17 (P4) and their diallel hybrids in horticultural research farm of Ferdowsi University of Mashhad, Iran in 2011. The measured traits were plant height at first flower, number of leaf to first flower, flower diameter, flower tube length, internode length, stem diameter, plant height at flowering stage, plant spreading, number of branches per plant, leaf length and width. The results of combining ability for all studied traits revealed that both additive and non-additive gene effects contributed to the inheritance of the traits. Estimates of general combining ability effects showed that parent P3 was a good general combiner for most of the studied traits. For flower diameter, hybrid combination P1 × P2 had the highest significant positive specific combining ability effects. Reciprocal effects were significant for all traits and hybrid combination P1 × P2 had the highest significant positive reciprocal effects for flower tube length and plant height. Heterosis was found significant relative to both the mid parent and batter parent for all traits. For flower diameter, the highest positive values of heterotic effects were recorded in hybrid combination P2 × P3 both relative to the parental mean (37.3%) and relative to the better parent (33.9%). It is obvious that heterotic effects represent an important resource in hybrid breeding of petunia.

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“…In order to select the best parents for hybridization, it is beneficial to measure both additive and nonadditive effects and also to understand the genetic interactions between lines (Kalloo et al 1974;Satija and Thukral 1985;Mebrahtu and Mohamed 2003).…”

Section: Generation Means and Heterosis Analysis Of Zinnia Elegansmentioning

confidence: 99%

“…Breeders can utilize this information to select the best parents and determine which breeding strategies will be effective for the improvement of specific traits. There are numerous reports where combining ability analysis has been used to describe the breeding value of parental lines with respect to their potential to produce hybrids, and also to provide basic genetic information on the inheritance of a given character in plant breeding (Reddy et al 2007;Borghi and Perenzin 1994;Dhaliwal and Gill 1973;Hegde et al 2007;Kalloo et al 1974;Kenga et al 2004;Krystkowiak et al 2009;Romanus et al 2008;Li et al 1997;Maris 1989;Mastebroek et al 2002;Satija and Thukral 1985;Shukla and Pandey 2008;Sprague and Tatum 1942;Stuber 1994;Mebrahtu and Mohamed 2003;Young and Virmani 1990). Although hybridization using male sterile lines is common practice in commercial applications of Zinnia elegans, there has been no report on the importance of combining ability and hybrid breeding of male sterile lines in the species.…”

Section: Introductionmentioning

confidence: 99%

Analysis of combining ability of two-types of male sterile and four restorer lines of Zinnia elegans

et al. 2010

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Eight cross combinations of Zinnia elegans were made using two recessive nuclear male sterile lines crossed with four restorers using the North Carolina Design II statistical method. Heterosis, combining ability and heritability was analysed using 12 horticultural traits and these demonstrated the advantage of heterosis in hybrid breeding of Zinnia elegans. Heterosis served to increase the number of whorls of ray florets across capitulum and the number of branches, and also decreased plant height, crown size, pedicel length and length of node. Thus, six horticultural traits were improved over mid parent and best parent status to fulfill major breeding goals of this herbaceous flower. The traits of plant height, number of whorls of ray florets across capitulum and pedicel length were primarily controlled by paternal additive effects, whereas crown size was mainly controlled by non-additive effects. Number of branches and length of node were affected both by paternal additive effects and non-additive effects. The ratio of general combining ability to specific combining ability indicated the importance of additive genes in the expression of these traits. Among the parental lines, AH003A and restorer A3 were chosen as primary female and male combiners, respectively. AH001A and restorer S5 were chosen as secondary combiners. The cross AH003A 9 A3 was determined as the most promising combination for producing potted plant characteristics, and AH001A 9 S5 was the best hybrid obtained in this study for cut flower traits. The analysis of combining ability for the parental lines showed that there was no causal relationship between general combining ability and specific combining ability effects.

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Genetic analysis of phytic acid content in pearl millet

Cited by 9 publications

References 9 publications

Temporal and Spatial Patterns of Accumulation of the Transcript of Myo-Inositol-1-Phosphate Synthase and Phytin-Containing Particles during Seed Development in Rice1

Temporal and Spatial Patterns of Accumulation of the Transcript of Myo-Inositol-1-Phosphate Synthase and Phytin-Containing Particles during Seed Development in Rice1

Estimation of Heterosis and Combining Ability in Petunia (Petunia hybrida Hort.)

Analysis of combining ability of two-types of male sterile and four restorer lines of Zinnia elegans

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