Genetic analysis of phenylpropanoid metabolites associated with resistance against Verticillium longisporum in Brassica napus

Obermeier, Christian; Hossain, M. A.; Snowdon, Rod J.; Knüfer, Jessica; Tiedemann, Andreas von; Friedt, Wolfgang

doi:10.1007/s11032-012-9794-8

Cited by 49 publications

(54 citation statements)

References 48 publications

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“…Functional analysis of the lignans and their glucosides might add these compounds to the list of anti‐fungal defense compounds. The importance of the phenylpropanoid pathway in the defense of the aerial plant parts against V. longisporum is also supported by studies on Brassica napus (Eynck et al ., ; Obermeier et al ., ). Here, soluble and cell wall‐bound phenolics seem to be involved in the early defense against the fungus, whereas lignin might be important at later infection stages (Eynck et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…In line with this, Obermeier et al . () found that quantitative trait loci (QTL) for some phenylpropanoids co‐localized with a QTL for V. longisporum resistance in B. napus . Concentrations of phenylpropanoids were correlated with V. longisporum resistance, making genes of the phenylpropanoid pathway interesting candidates for resistance breeding in oilseed rape (Obermeier et al ., ).…”

Section: Discussionmentioning

confidence: 99%

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Soluble phenylpropanoids are involved in the defense response of Arabidopsis against Verticillium longisporum

König¹,

Feussner²,

Kaever

et al. 2014

New Phytologist

117

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SummaryVerticillium longisporum is a soil-borne vascular pathogen causing economic loss in rape. Using the model plant Arabidopsis this study analyzed metabolic changes upon fungal infection in order to identify possible defense strategies of Brassicaceae against this fungus.Metabolite fingerprinting identified infection-induced metabolites derived from the phenylpropanoid pathway. Targeted analysis confirmed the accumulation of sinapoyl glucosides, coniferin, syringin and lignans in leaves from early stages of infection on. At later stages, the amounts of amino acids increased.To test the contribution of the phenylpropanoid pathway, mutants in the pathway were analyzed. The sinapate-deficient mutant fah1-2 showed stronger infection symptoms than wild-type plants, which is most likely due to the lack of sinapoyl esters. Moreover, the coniferin accumulating transgenic plant UGT72E2-OE was less susceptible. Consistently, sinapoyl glucose, coniferyl alcohol and coniferin inhibited fungal growth and melanization in vitro, whereas sinapyl alcohol and syringin did not. The amount of lignin was not significantly altered supporting the notion that soluble derivatives of the phenylpropanoid pathway contribute to defense.These data show that soluble phenylpropanoids are important for the defense response of Arabidopsis against V. longisporum and that metabolite fingerprinting is a valuable tool to identify infection-relevant metabolic markers.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Soluble phenylpropanoids are involved in the defense response of Arabidopsis against Verticillium longisporum

König¹,

Feussner²,

Kaever

et al. 2014

New Phytologist

117

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“…Here, we present a method which enabled us to accurately map agronomic QTL to a number of genomic rearrangement events. The 60K Illumina SNP array marker data were used to generate high‐density genetic maps in half‐sib DH populations from three synthetic B. napus accessions, carrying interesting disease resistance (Obermeier et al ., ), agronomic and yield‐related traits (Basunanda et al ., , ; Radoev et al ., ) and seed quality characters (Badani et al ., ; Stein et al ., ). Although the putative colocalization of HE events with QTL in B. napus has been suggested in previous studies (Chalhoub et al ., ; Liu et al ., ; Stein et al ., ) to our knowledge, this is the first study providing independent validation of QTL‐HE colocalization.…”

Section: Discussionmentioning

confidence: 99%

Mapping of homoeologous chromosome exchanges influencing quantitative trait variation in Brassica napus

Stein

Coriton

Rousseau‐Gueutin

et al. 2017

Plant Biotechnology Journal

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SummaryGenomic rearrangements arising during polyploidization are an important source of genetic and phenotypic variation in the recent allopolyploid crop Brassica napus. Exchanges among homoeologous chromosomes, due to interhomoeologue pairing, and deletions without compensating homoeologous duplications are observed in both natural B. napus and synthetic B. napus. Rearrangements of large or small chromosome segments induce gene copy number variation (CNV) and can potentially cause phenotypic changes. Unfortunately, complex genome restructuring is difficult to deal with in linkage mapping studies. Here, we demonstrate how high‐density genetic mapping with codominant, physically anchored SNP markers can detect segmental homoeologous exchanges (HE) as well as deletions and accurately link these to QTL. We validated rearrangements detected in genetic mapping data by whole‐genome resequencing of parental lines along with cytogenetic analysis using fluorescence in situ hybridization with bacterial artificial chromosome probes (BAC‐FISH) coupled with PCR using primers specific to the rearranged region. Using a well‐known QTL region influencing seed quality traits as an example, we confirmed that HE underlies the trait variation in a DH population involving a synthetic B. napus trait donor, and succeeded in narrowing the QTL to a small defined interval that enables delineation of key candidate genes.

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“…Current European oilseed rape cultivars possess a low level of Verticillium resistance, and the availability of novel germplasm for resistance breeding is limited because of the narrow genetic basis of currently used cultivars (Cowling, ; Seyis et al ., ). However, three quantitative trait loci (QTLs) that significantly correlate with V. longisporum resistance have been identified, one on the C1 and two on the C5 chromosome, of the partly resistant oilseed rape cultivar Express 617 (Obermeier et al ., ). These QTLs indicate sources of quantitative resistance available in the C‐genome derived from oilseed rape parental cabbage lines.…”

Section: Disease Managementmentioning

confidence: 97%

Verticillium longisporum, the invisible threat to oilseed rape and other brassicaceous plant hosts

Depotter

Deketelaere

Inderbitzin

et al. 2016

Molecular Plant Pathology

104

132

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Genetic analysis of phenylpropanoid metabolites associated with resistance against Verticillium longisporum in Brassica napus

Cited by 49 publications

References 48 publications

Soluble phenylpropanoids are involved in the defense response of Arabidopsis against Verticillium longisporum

Soluble phenylpropanoids are involved in the defense response of Arabidopsis against Verticillium longisporum

Mapping of homoeologous chromosome exchanges influencing quantitative trait variation in Brassica napus

Verticillium longisporum, the invisible threat to oilseed rape and other brassicaceous plant hosts

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