Genetic Analysis of Osmotic and Cold Stress Signal Transduction in Arabidopsis: Interactions and Convergence of Abscisic Acid-Dependent and Abscisic Acid-Independent Pathways

Ishitani, Manabu; Xiong, Liming; Stevenson, Becky; Zhu, Jian‐Kang

doi:10.2307/3870555

Cited by 233 publications

(361 citation statements)

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“…AtADH1 was reported to be a cold, dehydration, hypoxia, and ABA-inducible gene [52,53]. Regulation of the ADH1 gene in Arabidopsis by AtCBF2 is suggested by experiments, which alter accumulation of CBF2 [54][55][56] and overexpression of CBF2 [57]. Maruyama et al [17] suggested that the specific promoter elements regulated by DREB2 are ACCGAC, GTCGGT and CCGACT, while DREB1A/CBF3 regulated primarily ACCGAC.…”

Section: Gmdreb1a;2mentioning

confidence: 99%

Functionality of soybean CBF/DREB1 transcription factors

Yamasaki

Randall

2016

Plant Science

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Soybean (Glycine max) is considered to be cold intolerant and is not able to significantly acclimate to cold/freezing stress. In most cold tolerant plants, the Crepeat/DRE Binding Factors (CBF/DREBs) are critical contributors to successful cold-responses; rapidly increasing following cold treatment and regulating the induction of many cold responsive genes. In soybean vegetative tissue, we found strong, transient accumulation of CBF transcripts in response to cold stress; however, the soybean transcripts of typical cold responsive genes (homologues to Arabidopsis genes such as dehydrins, ADH1, RAP2.1, and LEA14) were not significantly altered.Soybean CBFs were found to be functional, as when expressed constitutively in Arabidopsis they increased the levels of AtCOR47 and AtRD29a transcripts and increased freezing tolerance as measured by a decrease in leaf freezing damage and ion leakage. Furthermore the constitutive expression of GmDREB1A;2 and GmDREB1B;1 in Arabidopsis led to stronger up-regulation of downstream genes and more freezing tolerance than GmDREB1A;1, the gene whose transcript is the major contributor to total CBF/DREB1 transcripts in soybean. The inability for the soybean CBFs to significantly up regulate the soybean genes that contribute to cold tolerance is consistent with poor acclimation capability and the cold intolerance of soybean.

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Section: Gmdreb1a;2mentioning

confidence: 99%

Functionality of soybean CBF/DREB1 transcription factors

Yamasaki

Randall

2016

Plant Science

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“…Some of these encode proteins responsible for the biosynthesis of hormones, such as abscisic acid (ABA), which can act as signaling molecules that amplify and spread the initial stress signal. Interestingly, different stresses as well as ABA treatment can change the expression of a common set of genes, indicating that stress responses are mediated in part by overlapping signaling pathways (Ishitani et al, 1997;Shinozaki and Yamaguchi-Shinozaki, 2000;Rabbani et al, 2003). However, these common signaling pathways might be activated in a different temporal and spatial manner by individual stresses (Xiong et al, 2002;Delessert et al, 2004;Kilian et al, 2007;Dinneny et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Stress‐induced changes in the Arabidopsis thaliana transcriptome analyzed using whole‐genome tiling arrays

et al. 2009

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SUMMARYThe responses of plants to abiotic stresses are accompanied by massive changes in transcriptome composition. To provide a comprehensive view of stress-induced changes in the Arabidopsis thaliana transcriptome, we have used whole-genome tiling arrays to analyze the effects of salt, osmotic, cold and heat stress as well as application of the hormone abscisic acid (ABA), an important mediator of stress responses. Among annotated genes in the reference strain Columbia we have found many stress-responsive genes, including several transcription factor genes as well as pseudogenes and transposons that have been missed in previous analyses with standard expression arrays. In addition, we report hundreds of newly identified, stress-induced transcribed regions. These often overlap with known, annotated genes. The results are accessible through the Arabidopsis thaliana Tiling Array Express (At-TAX) homepage, which provides convenient tools for displaying expression values of annotated genes, as well as visualization of unannotated transcribed regions along each chromosome.

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“…To cope with such unfavorable growth conditions, plants have developed unique defense mechanisms and processes for acclimation that enhance their tolerance to detrimental conditions. A number of genetic and cellular events that occur under such stress have been widely documented (Bray, 1997;Ishitani et al, 1997;Zhu, 2002). Diverse sets of genes induced by drought and/or salt stress have been identified recently with the aid of combined molecular and genetic approaches (Ingram and Bartels, 1996;Cushman and Bohnert, 2000;Seki et al, 2001Seki et al, , 2002Ozturk et al, 2002;Oono et al, 2003).…”

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confidence: 99%

Heterologous Expression and Molecular and Cellular Characterization of CaPUB1 Encoding a Hot Pepper U-Box E3 Ubiquitin Ligase Homolog

et al. 2006

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The U-box motif is a conserved domain found in the diverse isoforms of E3 ubiquitin ligase in eukaryotes. From water-stressed hot pepper (Capsicum annuum L. cv Pukang) plants, we isolated C. annuum putative U-box protein 1 (CaPUB1), which encodes a protein containing a single U-box motif in its N-terminal region. In vitro ubiquitination and site-directed mutagenesis assays revealed that CaPUB1 possessed E3 ubiquitin ligase activity and that the U-box motif was indeed essential for its enzyme activity. RNA gel-blot analysis showed that CaPUB1 mRNA was induced rapidly by a broad spectrum of abiotic stresses, including drought, high salinity, cold temperature, and mechanical wounding, but not in response to ethylene, abscisic acid, or a bacterial pathogen, suggesting its role in the early events in the abiotic-related defense response. Because transgenic work was extremely difficult in hot pepper, in this study we overexpressed CaPUB1 in Arabidopsis (Arabidopsis thaliana) to provide cellular information on the function of this gene in the development and plant responses to abiotic stresses. Transgenic Arabidopsis plants that constitutively expressed the CaPUB1 gene under the control of the cauliflower mosaic virus 35S promoter had markedly longer hypocotyls and roots and grew more rapidly than the wild type, leading to an early bolting phenotype. Microscopic analysis showed that 35STCaPUB1 roots had increased numbers of small-sized cells, resulting in disordered, highly populated cell layers in the cortex, endodermis, and stele. In addition, CaPUB1-overexpressing plants displayed increased sensitivity to water stress and mild salinity. These results indicate that CaPUB1 is functional in Arabidopsis cells, thereby effectively altering cell and tissue growth and also the response to abiotic stresses. Comparative proteomic analysis showed that the level of RPN6 protein, a non-ATPase subunit of the 26S proteasome complex, was significantly reduced in 35STCaPUB1 seedlings as compared to the wild type. Pull-down and ubiquitination assays demonstrated that RPN6 interacted physically with CaPUB1 and was ubiquitinated in a CaPUB1-dependent manner in vitro. Although the physiological function of CaPUB1 is not yet clear, there are several possibilities for its involvement in a subset of physiological responses to counteract dehydration and high-salinity stresses in transgenic Arabidopsis seedlings.

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Genetic Analysis of Osmotic and Cold Stress Signal Transduction in Arabidopsis: Interactions and Convergence of Abscisic Acid-Dependent and Abscisic Acid-Independent Pathways

Cited by 233 publications

References 0 publications

Functionality of soybean CBF/DREB1 transcription factors

Functionality of soybean CBF/DREB1 transcription factors

Stress‐induced changes in the Arabidopsis thaliana transcriptome analyzed using whole‐genome tiling arrays

Heterologous Expression and Molecular and Cellular Characterization of CaPUB1 Encoding a Hot Pepper U-Box E3 Ubiquitin Ligase Homolog

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