Genetic Analysis of Mps3 SUN Domain Mutants in<i>Saccharomyces cerevisiae</i>Reveals an Interaction with the SUN-Like Protein Slp1

Friederichs, Jennifer M.; Gardner, Jennifer M.; Smoyer, Christine J.; Whetstine, Christine R.; Gogol, Madelaine; Slaughter, Brian D.; Jaspersen, Sue L.

doi:10.1534/g3.112.004614

Cited by 33 publications

(40 citation statements)

References 86 publications

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“…Perhaps, the SPB has a role in controlling lipid homeostasis in the NE as part of a normal coordination of events during cell division. Previous work indicates that the SPB protein Mps3 is involved in NE homeostasis in addition to its role in SPB duplication 37 , 42 . Although we found that two Mps3 ts mutations, mps3–1 -and mps3–7 , also affect Trm1-II-GFP location, our work suggests a broader role for the SPB in NE homeostasis.…”

Section: Discussioncontrasting

confidence: 56%

Requirement of the spindle pole body for targeting and/or tethering proteins to the inner nuclear membrane

Diaz-Muñoz

Harchar

Lai

et al. 2014

Nucleus

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Appropriate targeting of inner nuclear membrane (INM) proteins is important for nuclear function and architecture. To gain new insights into the mechanism(s) for targeting and/or tethering peripherally associated proteins to the INM, we screened a collection of temperature sensitive S. cerevisiae yeast mutants for defects in INM location of the peripheral protein, Trm1-II-GFP. We uncovered numerous genes encoding components of the Spindle Pole Body (SPB), the yeast centrosome. SPB alterations affect the localization of both an integral (Heh2) and a peripheral INM protein (Trm1-II-GFP), but not a nucleoplasmic protein (Pus1). In wild-type cells Trm1-II-GFP is evenly distributed around the INM, but in SPB mutants, Trm1-II-GFP mislocalizes as a spot(s) near ER-nucleus junctions, perhaps its initial contact site with the nuclear envelope. Employing live cell imaging over time in a microfluidic perfusion system to study protein dynamics, we show that both Trm1-II-GFP INM targeting and maintenance depend upon the SPB. We propose a novel targeting and/or tethering model for a peripherally associated INM protein that combines mechanisms of both integral and soluble nuclear proteins, and describe a role of the SPB in nuclear envelope dynamics that affects this process.

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Section: Discussioncontrasting

confidence: 56%

Requirement of the spindle pole body for targeting and/or tethering proteins to the inner nuclear membrane

Diaz-Muñoz

Harchar

Lai

et al. 2014

Nucleus

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“…These findings expand also the known roles of the mid-SUN-domain proteins. The other known members of this family show similar ER/NE localization and have been implicated in a diversity of processes, including folding of integral membrane proteins in budding yeast (15,16), cell proliferation and differentiation in amoeba (14), regulation of bone formation in mouse (13,17), and dehydration signaling in chickpea (30). Sordaria represents thus the first instance, to our knowledge, in which a member of this family is implicated in karyogamy.…”

Section: Discussionmentioning

confidence: 94%

“…The precise role of those proteins remains unknown. Budding yeast Slp1 is implicated in folding of integral membrane proteins and is involved directly or indirectly in Sun1/Mps3 localization to the NE (15,16). Dictyostellum discoideum SunB protein plays roles in both cell proliferation and differentiation (14).…”

Section: Significancementioning

confidence: 99%

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Absence of SUN-domain protein Slp1 blocks karyogamy and switches meiotic recombination and synapsis from homologs to sister chromatids

Vasnier

Muyt

Zhang

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Karyogamy, the process of nuclear fusion is required for two haploid gamete nuclei to form a zygote. Also, in haplobiontic organisms, karyogamy is required to produce the diploid nucleus/cell that then enters meiosis. We identify sun like protein 1 (Slp1), member of the mid-Sad1p, UNC-84-domain ubiquitous family, as essential for karyogamy in the filamentous fungus Sordaria macrospora, thus uncovering a new function for this protein family. Slp1 is required at the last step, nuclear fusion, not for earlier events including nuclear movements, recognition, and juxtaposition. Correspondingly, like other family members, Slp1 localizes to the endoplasmic reticulum and also to its extensions comprising the nuclear envelope. Remarkably, despite the absence of nuclear fusion in the slp1 null mutant, meiosis proceeds efficiently in the two haploid "twin" nuclei, by the same program and timing as in diploid nuclei with a single dramatic exception: the normal prophase program of recombination and synapsis between homologous chromosomes, including loading of recombination and synaptonemal complex proteins, occurs instead between sister chromatids. Moreover, the numbers of recombinationinitiating double-strand breaks (DSBs) and ensuing recombinational interactions, including foci of the essential crossover factor Homo sapiens enhancer of invasion 10 (Hei10), occur at half the diploid level in each haploid nucleus, implying per-chromosome specification of DSB formation. Further, the distribution of Hei10 foci shows interference like in diploid meiosis. Centromere and spindle dynamics, however, still occur in the diploid mode during the two meiotic divisions. These observations imply that the prophase program senses absence of karyogamy and/or absence of a homolog partner and adjusts the interchromosomal interaction program accordingly.sisters versus homologs | recombination/synapsis K aryogamy is the process by which two nuclei fuse to produce a single nucleus. This process is critical in diploid organisms (e.g., mammals and plants) when haploid egg and sperm nuclei fuse to produce a diploid nucleus and zygote. In organisms with a haploid vegetative cycle (e.g., fungi and most algae), karyogamy is required to produce the diploid nucleus/cell, which will then enter meiosis. In both situations, karyogamy involves two steps: nuclear movement leading to nuclear juxtaposition (congression) and final fusion of the nuclear membranes. Cooperation of the cytoskeleton components is required for nuclear movement and correct positioning of the nuclei (e.g., ref. 1).In budding yeast, premeiotic karyogamy requires several genes (named Kar1 to Kar9) with their mutant defects corresponding to two steps: nuclear congression, which involves cytoskeleton components, motor proteins and the spindle pole body (SPB, mammal centrosome equivalent) and fusion of the two haploid nuclear envelopes (NEs), which involves either the endoplasmic reticulum (ER) per se or protein translocation into the perinuclear space (reviewed in refs. 2-4 and references t...

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“…This has recently been supported by the characterization of the S. cerevisiae mid-SUN protein SLP1 (SUN-like protein) and its interaction with the classical C-terminal yeast SUN protein Msp3. 13 Findings presented by several participants (Bass, Graumann and Tatout) on the previously uncharacterized mid-SUN proteins provide an example in which the plant field leads research efforts to identify and characterize novel nuclear envelope components. Both Graumann and Christophe Tatout (Blaise Pascal University, France) presented information about the molecular interactions of Arabidopsis SUN proteins.…”

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confidence: 99%

SUNrises on the International Plant Nucleus Consortium

Graumann

Bass

Parry

2013

Nucleus

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Genetic Analysis of Mps3 SUN Domain Mutants inSaccharomyces cerevisiaeReveals an Interaction with the SUN-Like Protein Slp1

Cited by 33 publications

References 86 publications

Requirement of the spindle pole body for targeting and/or tethering proteins to the inner nuclear membrane

Requirement of the spindle pole body for targeting and/or tethering proteins to the inner nuclear membrane

Absence of SUN-domain protein Slp1 blocks karyogamy and switches meiotic recombination and synapsis from homologs to sister chromatids

SUNrises on the International Plant Nucleus Consortium

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