Genetic analysis of microsatellites associated with resistance against bacterial leaf blight and blast diseases of rice (<i>Oryza sativa</i> L.)

Chukwu, Samuel Chibuike; Rafii, Mohd Y.; Ramlee, Shairul Izan; Ismail, Siti Izera; Oladosu, Yusuff; Muhammad, Isma’ila; Ubi, Benjamin Ewa; Nwokwu, G. N.

doi:10.1080/13102818.2020.1809520

Cited by 9 publications

(6 citation statements)

References 33 publications

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“…Our results met the expectations based on common genetic knowledge. Similar data were reported for rice, wheat, and maize 39 – 41 . As a side effect, our results demonstrate the accurateness of hand crosses because we found no self-pollination events.…”

Section: Discussionsupporting

confidence: 86%

Genomic background selection to reduce the mutation load after random mutagenesis

Karunarathna

Patiranage

Harloff

et al. 2021

Sci Rep

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Random mutagenesis is a standard procedure to increase allelic variation in a crop species, especially in countries where the use of genetically modified crops is limited due to legal constraints. The chemical mutagen EMS is used in many species to induce random mutations throughout the genome with high mutation density. The major drawback for functional analysis is a high background mutation load in a single plant that must be eliminated by subsequent backcrossing, a time and resource-intensive activity. Here, we demonstrate that genomic background selection combined with marker-assisted selection is an efficient way to select individuals with reduced background mutations within a short period. We identified BC1 plants with a significantly higher share of the recurrent parent genome, thus saving one backcross generation. Furthermore, spring rapeseed as the recurrent parent in a backcrossing program could accelerate breeding by reducing the generation cycle. Our study depicts the potential for reducing the background mutation load while accelerating the generation cycle in EMS-induced winter oilseed rape populations by integrating genomic background selection.

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Section: Discussionsupporting

confidence: 86%

Genomic background selection to reduce the mutation load after random mutagenesis

Karunarathna

Patiranage

Harloff

et al. 2021

Sci Rep

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“…The current study tested five sets of molecular markers that had previously shown functional polymorphisms for each of the target traits, including Wx -SSR, fgr -SNP, pTA248, RM8225, and RM202 [ 19 , 20 , 21 , 22 , 23 , 24 ]. All of the selected markers showed functional polymorphisms with expected sizes of amplified products ( Table 5 ), indicating that they can be employed for multiplexing.…”

Section: Resultsmentioning

confidence: 99%

Novel PCR-Based Multiplex Assays for Detecting Major Quality and Biotic Stress in Commercial and Weedy Rice

Hanafiah

Cheng

Lim

et al. 2022

Life

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While previous research has demonstrated that multiplex polymerase chain reaction (PCR) can be a cost-effective approach to detect various genes in crops, the availability of multiplex assays to simultaneously screen both grain quality and biotic stress resistance traits in rice (Oryza sativa) is limited. In this work, we report six novel multiplex assays that use a universal protocol to detect major rice grain quality (amylose content and fragrance) and biotic stress (blast, sheath blight, and bacterial leaf blight) traits with amplified products consisting of up to four primer pairs that can be analyzed using a standard agarose-based gel electrophoresis system. Recent studies have suggested that weedy rice has novel sources of disease resistance. However, an intensive screening of weedy biotypes has not been reported in Malaysia. Accordingly, we employed one of the developed multiplex assays to screen reported genes or quantitative trait loci (QTLs) associated with blast, sheath blight, and bacterial leaf blight diseases in 100 weedy rice biotypes collected from five local fields, with phenotyping performed to validate the genotyping results. In conclusion, our universal multiplex protocol is effective for the large-scale genotyping of rice genetic resources, and it can be employed in routine molecular laboratories with limited resources.

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“…Genes such as Ammonium transport (AMT), nitrate transport (NRT), glutamine synthetase (GS), and glutamate synthase (GOGAT) play pivotal roles in nitrogen metabolism. Studies have demonstrated that transgenic crops overexpressing these genes exhibit elevated tissue nitrogen levels, increased amino acids, and enhanced biomass and greater seed production (Curatti and Rubio, 2014). For instance, the gene OsDREB1C, responsible for promoting nitrogen use efficiency and resource allocation while shortening growth, has led to a substantial increase in rice yield, ranging from 41.3% to 68.3% compared to wild types when overexpressed (Wei et al, 2022).…”

Section: Enhancing Physiological Efficiency Of Plantsmentioning

confidence: 99%

Synergizing biotechnology and natural farming: pioneering agricultural sustainability through innovative interventions

Badiyal,

Mahajan,

Rana

et al. 2024

Front. Plant Sci.

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The world has undergone a remarkable transformation from the era of famines to an age of global food production that caters to an exponentially growing population. This transformation has been made possible by significant agricultural revolutions, marked by the intensification of agriculture through the infusion of mechanical, industrial, and economic inputs. However, this rapid advancement in agriculture has also brought about the proliferation of agricultural inputs such as pesticides, fertilizers, and irrigation, which have given rise to long-term environmental crises. Over the past two decades, we have witnessed a concerning plateau in crop production, the loss of arable land, and dramatic shifts in climatic conditions. These challenges have underscored the urgent need to protect our global commons, particularly the environment, through a participatory approach that involves countries worldwide, regardless of their developmental status. To achieve the goal of sustainability in agriculture, it is imperative to adopt multidisciplinary approaches that integrate fields such as biology, engineering, chemistry, economics, and community development. One noteworthy initiative in this regard is Zero Budget Natural Farming, which highlights the significance of leveraging the synergistic effects of both plant and animal products to enhance crop establishment, build soil fertility, and promote the proliferation of beneficial microorganisms. The ultimate aim is to create self-sustainable agro-ecosystems. This review advocates for the incorporation of biotechnological tools in natural farming to expedite the dynamism of such systems in an eco-friendly manner. By harnessing the power of biotechnology, we can increase the productivity of agro-ecology and generate abundant supplies of food, feed, fiber, and nutraceuticals to meet the needs of our ever-expanding global population.

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Genetic analysis of microsatellites associated with resistance against bacterial leaf blight and blast diseases of rice (Oryza sativa L.)

Cited by 9 publications

References 33 publications

Genomic background selection to reduce the mutation load after random mutagenesis

Genomic background selection to reduce the mutation load after random mutagenesis

Novel PCR-Based Multiplex Assays for Detecting Major Quality and Biotic Stress in Commercial and Weedy Rice

Synergizing biotechnology and natural farming: pioneering agricultural sustainability through innovative interventions

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