Genetic analysis of inbreeding depression in plus tree 850.55 of Pinus radiata D. Don. I. Genetic map with distorted markers

Kuang, Hanhui; Richardson, Thomas E.; Carson, S. D.; Wilcox, Phillip; Bongarten, B. C.

doi:10.1007/s001220051123

Cited by 51 publications

(44 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies in the same species have detected up to 34 % of segregation distortion (Kuang et al 1999), however the smaller number of offspring compared to earlier studies (Wilcox et al 2001, Devey et al 2004 could be responsible for the low representativeness of the expected allelic proportions.…”

Section: Resultsmentioning

confidence: 61%

“…Polymorphic information, mode of inheritance, genomic size and complexity are factors that influence the development of a particular technique and the selection of informative molecular markers (Plomion et al 2007). Among the molecular techniques implemented for P. radiata are restriction fragment length polymorphism (RFLP; Devey et al 2004), random amplified polymorphic DNA (RAPD; Kuang et al 1999, Wilcox et al 2001, amplified fragment length polymorphism (AFLP; Wilcox et al 2001), simple sequence repeat (SSR; Fisher et al 1998, Devey et al 2002, Chagné et al 2004) and single-nucleotide polymorphism (SNP; Dillon et al 2010).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata

Moraga-Suazo¹,

Hasbún²,

Balocchi³

et al. 2012

Bosque (Valdivia)

View full text Add to dashboard Cite

SUMMARYPinus radiata is a conifer of recognized economic value. Studies at genetic and molecular level to support breeding programs of radiata pine have been conducted with difficulty due to its large and complex genome. The development of molecular markers has allowed performing studies on genetic variation, identification of clones and construction of linkage maps. In order to increase the genetic molecular markers in P. radiata, two inter simple sequence repeat (ISSR) markers and twelve selective amplifications of microsatellite polymorphic loci (SAMPL) primer combinations were tested, using a first-generation full-sib family of 86 individuals. Polymerase chain reaction (PCR) products were visualized by capillary electrophoresis and polymorphism was detected by presence or absence of a particular fragment in one P. radiata parental. A total of 18 polymorphic fragments were found for two ISSR primers tested, with an average segregation distortion of 33 %. SAMPL markers yielded 85 polymorphic fragments with eight primer combinations, with an average of 22.3 % segregation distortion. Both methodologies showed a good reproducibility, with easy implementation and useful for genetic analysis on P. radiata.Key words: capillary electrophoresis, segregation distortion, forest trees. RESUMENPinus radiata es una conífera de reconocido valor económico, en la cual los estudios a nivel genético y molecular, han sido conducidos con dificultad en programas de mejoramiento debido a su genoma complejo y de gran tamaño. El desarrollo de marcadores moleculares ha permitido realizar estudios de variación genética, validar clones y construir mapas de ligamiento. Para lograr incrementar la disponibilidad de marcadores moleculares en P. radiata, se examinaron dos marcadores ISSR (inter simple sequence repeat) y 12 combinaciones de partidores SAMPL (selective amplification of microsatellite polymorphic loci), usando una familia F1 de 86 hermanos completos de primera generación. Los productos de PCR (polymerase chain reaction) se visualizaron mediante electroforesis capilar y la selección de fragmentos polimórficos se efectuó a base de la presencia o ausencia de un fragmento en uno de los parentales de la familia de P. radiata. Un total de 18 fragmentos polimórficos fueron encontrados para los dos partidores ISSR, con una distorsión de segregación promedio de 33 %. Los marcadores SAMPL generaron 85 fragmentos polimórficos empleando ocho combinaciones, con un promedio de 22,3 % de distorsión de segregación. Ambas metodologías mostraron buena reproducibilidad, fácil implementación y utilidad para análisis genético en P. radiata.Palabras clave: electroforesis capilar, distorsión de segregación, especies forestales.

show abstract

Section: Resultsmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata

Moraga-Suazo¹,

Hasbún²,

Balocchi³

et al. 2012

Bosque (Valdivia)

View full text Add to dashboard Cite

show abstract

“…In more species chromosomal information is need for understand of species relationships in respect to chromosomes in a large genus Pinus. On the other hand the genome studies are progressing in several pine species of P. radiate (Kuang et al 1999), P. thumbergii (Kondo et al 2000), P. contorta (Li and Yeh 2001), P. elliottii (Brown et al 2001), P. pinaster (Chagné et al 2003), P. sylvestris (Hurne et al 2000;Komulainen et al 2003), P. densiflora (Kim et al 2005), P. taeda (Echt et al 2011;Martínez-García et al 2013), P. lambertiana (Jermstad et al 2011) and P. balfouriana (Friedline et al 2015) and their genome maps are constructed by various molecular markers such as AFLPs, RFLPs, RAPDs, ESTPs, cDNA and SSRs and compared among species in respect to synteny (see Jermstad et al 2011). In pine genome study correspondence between individual chromosomes and linkage groups of genetic map is not established in Pinus.…”

Section: Abstract: Chromosome Diploxylon Pine Fluorescent Band Karmentioning

confidence: 99%

Chromosome banding in the genus <i>Pinus</i> V. Fluorescent banding patterns in 16 diploxylon pines

et al. 2016

View full text Add to dashboard Cite

ABSTRACT:In Pinus , Subgenus Pinus 16 species were investigated on their somatic chromosomes by a fluorescent banding technique using chromomycin A 3 (CMA) and 4',6-diamidino-2-phenylindole (DAPI). The chromosome number of 2n=24 was commonly counted in all the species studied. Their karyotypes were composed of many long metacentric chromosomes and a few short submetacentric chromosomes, and two karyotypes were recognized in respect to number of short chromosomes in the subgenus. CMA-bands appeared at an interstitial and/or proximal region of chromosomes and DAPI-band did at a proximal region. Thin DAPI-bands appeared at interstitial regions and DAPI-dots appeared at a centromeric region in most chromosomes. In a chromosome complement each homologous chromosome was identified on the base of its shape and fluorescent banding pattern. The typical banding patterns were compared among the species studied. Many interstitial CMA-bands at the secondary constrictions appeared on many metacentric chromosomes. Proximal fluorescent bands varied in fluorescent nature and number and divided into several groups. Section Pinus short three chromosome pairs were two or three patterns of fluorescent banding. The section Trifolae species had many proximal DAPI-bands and less proximal CMA-bands than section Pinus. The shortest chromosome had proximal bands indicating two groups on the kinds of fluorescent band.

show abstract

“…Nevertheless, 32% of the loci presented segregation distortion. Polymorphisms that showed significant departures from Mendelian expectations have been reported for all types of markers (RFLP, RAPD, SCAR, AFLP, microsatellite, isoenzyme) used in crops and forest trees (Aravanopoulos et al, 1993;Kuang et al, 1999), but the causes of this phenomenon are not well understood. The percentage of loci showing segregation distortion is highly variable in forest trees: 69% in Cryptomeria japonica (Nikaido et al, 1999), 20% in Quercus robur (Barreneche et al, 1998), 18% in Pseudotsuga menziesii (Krutovskii et al, 1998), 14% in Populus (Bradshaw et al, 1994), and 15% in Salix exigua (Aravanopoulos et al, 1993).…”

Section: Marker Polymorphism and Segregationmentioning

confidence: 99%

“…Any distorted markers were eliminated from the dataset. Kuang et al (1999) showed that recombination frequency is not affected by the segregation distortion caused by a viability gene or sampling error. Nevertheless, distorted markers were eliminated based on low marker coverage of the genome and low number of progeny.…”

Section: Linkage Analysis and Map Constructionmentioning

confidence: 99%

Linkage mapping of the Mediterranean cypress, Cupressus sempervirens, based on molecular and morphological markers

Hamamouch¹,

Maios²,

Harfouche³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Gene mapping for a Cupressus species is presented for the first time. Two linkage maps for the Mediterranean cypress (Cupressus sempervirens) varieties, C. sempervirens var. horizontalis and C. sempervirens var. pyramidalis, were constructed following the pseudo-testcross mapping strategy and employing RAPD, SCAR and morphological markers. A total of 427 loci (425 RAPDs, two SCARs) representing parents and F 1 progeny were screened for polymorphism with 32 random decamer and two SCAR primers. A morphological marker defined as "crown form" was also included. Of 274 polymorphic loci, the 188 that presented Mendelian inheritance formed the mapping dataset. Of these loci, 30% were mapped into seven linkage groups for the horizontalis (maternal) and four linkage groups for the pyramidalis (paternal) map. The putative "crown form" locus was included in a linkage group of both maps. The horizontalis and the pyramidalis maps covered 160.1 and 144.5 cM, respectively, while genome length was estimated to be 1696 cM for the former variety and 1373 cM for the latter. The four RAPD markers most tightly linked to crown form were cloned and converted to SCARs. Each of the cloned RAPD markers yielded two to three different sequences behaving as co-migrating fragments. Two SCAR markers, SC-D05 432 and SC-D09 667 , produced amplified bands of the expected sizes and maintained linkage with the appropriate phenotype, but to a lesser extent compared to their original RAPD counterparts. These linkage maps represent a first step towards the localization of QTLs and genes controlling crown form and other polygenic traits in cypress.

show abstract

Genetic analysis of inbreeding depression in plus tree 850.55 of Pinus radiata D. Don. I. Genetic map with distorted markers

Cited by 51 publications

References 25 publications

Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata

Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata

Chromosome banding in the genus <i>Pinus</i> V. Fluorescent banding patterns in 16 diploxylon pines

Linkage mapping of the Mediterranean cypress, Cupressus sempervirens, based on molecular and morphological markers

Contact Info

Product

Resources

About