Genetic Analysis of
            <i>NDT80</i>
            Family Transcription Factors in
            <i>Candida albicans</i>
            Using New CRISPR-Cas9 Approaches

Min, Kyung-Hee; Biermann, Amy R.; Hogan, Deborah A.; Konopka, James B.

doi:10.1128/msphere.00545-18

Cited by 43 publications

(54 citation statements)

References 47 publications

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“…This result is in contrast to the findings in a recent study, where the deletion of RON1 using the CRISPR/Cas9 method did not affect filamentous growth in response to GlcNAc [28]. However, other phenotypes were indeed observed for the ron1/ron1 mutant strain in this study that were consistent with its role in GlcNAc catabolism [28]. Moreover, the ndt80/ndt80 ron1/ron1 double mutant strain exhibited increased filamentation in the presence of GlcNAc.…”

Section: Glcnac Catabolismcontrasting

confidence: 99%

“…Another transcription factor, Ron1, was discovered to regulate GlcNAc catabolism and GlcNAc-induced filamentous growth in C. albicans [24]. This result is in contrast to the findings in a recent study, where the deletion of RON1 using the CRISPR/Cas9 method did not affect filamentous growth in response to GlcNAc [28]. However, other phenotypes were indeed observed for the ron1/ron1 mutant strain in this study that were consistent with its role in GlcNAc catabolism [28].…”

Section: Glcnac Catabolismcontrasting

confidence: 78%

“…Moreover, the ndt80/ndt80 ron1/ron1 double mutant strain exhibited increased filamentation in the presence of GlcNAc. Taken together, these results suggest that Ron1 could function as a negative regulator of filamentation in the absence of Ndt80 [28]. The discrepancies between the two studies could be due to secondary mutations that may have arisen during construction of the different ron1/ron1 mutant strains used.…”

Section: Glcnac Catabolismmentioning

confidence: 87%

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N-Acetylglucosamine (GlcNAc) Sensing, Utilization, and Functions in Candida albicans

Ennis

Hernday

et al. 2020

JoF

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The sensing and efficient utilization of environmental nutrients are critical for the survival of microorganisms in environments where nutrients are limited, such as within mammalian hosts. Candida albicans is a common member of the human microbiota as well as an opportunistic fungal pathogen. The amide derivative sugar N-acetlyglucosamine (GlcNAc) is an important signaling molecule for C. albicans that could be a major nutrient source for this fungus in host settings. In this article, we review progress made over the past two decades on GlcNAc utilization, sensing, and functions in C. albicans and its related fungal species. GlcNAc sensing and catabolic pathways have been intensively studied in C. albicans. The C. albicans protein Ngt1 represents the first identified GlcNAc-specific transporter in eukaryotic organisms. In C. albicans, GlcNAc not only induces morphological transitions including the yeast to hyphal transition and the white to opaque phenotypic switch, but it also promotes fungal cell death. The Ras-cAMP/PKA signaling pathway plays critical roles in regulating these processes. Given the importance of GlcNAc sensing and utilization in C. albicans, targeting GlcNAc associated pathways and key pathway components could be promising in the development of new antifungal strategies.

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Section: Glcnac Catabolismcontrasting

confidence: 99%

Section: Glcnac Catabolismcontrasting

confidence: 78%

Section: Glcnac Catabolismmentioning

confidence: 87%

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N-Acetylglucosamine (GlcNAc) Sensing, Utilization, and Functions in Candida albicans

Ennis

Hernday

et al. 2020

JoF

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“…Subsequent rounds of marker recycling enable the deletion of multiple genes within a single strain using only 2 marker types. It is also possible to use traditional recyclable markers as repair constructs in CRISPR-based deletion schemes [17].…”

Section: Crispr/cas9 Cuts the Time To A C Albicans Double Mutant Bymentioning

confidence: 99%

Genetic interaction analysis comes to the diploid human pathogen Candida albicans

Glazier

Krysan

2020

PLoS Pathog

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“…In addition, as described above, a mutant that cannot catabolize GlcNAc can be stimulated by GlcNAc to form hyphae without obvious induction of hyphal-specific genes [23]. Other studies have shown that some transcription key factors needed for hyphal growth can be bypassed under special conditions [59][60][61]. Thus, although it is clear that transcriptional regulation is required to make cells competent to be induced to form hyphae, there is no clear evidence at this point that the hyphal-induced genes promote hyphal growth, which has led to development of alternative models, such as translational regulation [57].…”

Section: Hyphal-induced Genes Do Not Play An Obvious Role In Hyphal Mmentioning

confidence: 99%

N-Acetylglucosamine Regulates Morphogenesis and Virulence Pathways in Fungi

Min

Naseem

Konopka

2019

JoF

Self Cite

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N-acetylglucosamine (GlcNAc) is being increasingly recognized for its ability to stimulate cell signaling. This amino sugar is best known as a component of cell wall peptidoglycan in bacteria, cell wall chitin in fungi and parasites, exoskeletons of arthropods, and the extracellular matrix of animal cells. In addition to these structural roles, GlcNAc is now known to stimulate morphological and stress responses in a wide range of organisms. In fungi, the model organisms Saccharomyces cerevisiae and Schizosaccharomyces pombe lack the ability to respond to GlcNAc or catabolize it, so studies with the human pathogen Candida albicans have been providing new insights into the ability of GlcNAc to stimulate cellular responses. GlcNAc potently induces C. albicans to transition from budding to filamentous hyphal growth. It also promotes an epigenetic switch from White to Opaque cells, which differ in morphology, metabolism, and virulence properties. These studies have led to new discoveries, such as the identification of the first eukaryotic GlcNAc transporter. Other results have shown that GlcNAc can induce signaling in C. albicans in two ways. One is to act as a signaling molecule independent of its catabolism, and the other is that its catabolism can cause the alkalinization of the extracellular environment, which provides an additional stimulus to form hyphae. GlcNAc also induces the expression of virulence genes in the C. albicans, indicating it can influence pathogenesis. Therefore, this review will describe the recent advances in understanding the role of GlcNAc signaling pathways in regulating C. albicans morphogenesis and virulence.

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Genetic Analysis of NDT80 Family Transcription Factors in Candida albicans Using New CRISPR-Cas9 Approaches

Cited by 43 publications

References 47 publications

N-Acetylglucosamine (GlcNAc) Sensing, Utilization, and Functions in Candida albicans

N-Acetylglucosamine (GlcNAc) Sensing, Utilization, and Functions in Candida albicans

Genetic interaction analysis comes to the diploid human pathogen Candida albicans

N-Acetylglucosamine Regulates Morphogenesis and Virulence Pathways in Fungi

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