Genetic analysis of hydratidiform moles utilizing the oligonucleotide-DNA typing of the HLA-DRB gene

Yabe, Noriko; Maeda, Takahiro; Kashiwagi, Noboru; Obata, Fumiya

doi:10.1016/s0143-4004(05)80422-9

Placenta

1994

DOI: 10.1016/s0143-4004(05)80422-9

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Genetic analysis of hydratidiform moles utilizing the oligonucleotide-DNA typing of the HLA-DRB gene

Noriko Yabe

Takahiro Maeda

Noboru Kashiwagi

et al.

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1997

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“…Conversely, partial HM are most often triploid, containing one set of maternal genes together with two sets from the father.5 Karyotyping can, therefore, provide useful information regarding the genetic constitution of HM but its usefulness may be limited by the absence of suitable markers with which to assign maternal or paternal origin to molar chromosomes. 6 Flow cytometry has been used as an adjunct to the classification of HM, based on the difference in DNA ploidy observed within complete and partial HM.2…”

mentioning

confidence: 99%

Genetic analysis of hydatidiform moles in paraffin wax embedded tissue using rapid, sequence specific PCR-based HLA class II typing.

Bateman

Hemmatpour²,

Theaker³

et al. 1997

Journal of Clinical Pathology

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Aims-To determine the applicability of rapid, sequence specific polymerase chain reaction (PCR)-based HLA class II genotyping for the distinction ofcomplete from partial hydatidiform moles (HM) using DNA extracted from formalin fixed and paraffin wax embedded tissue. Methods-Nine HM were studied. DNA was extracted from formalin fixed and paraffin wax embedded tissue after mechanical separation of decidual and molar components. HLA class II DRB (DRB1, -3, -4, and -5) and DQB1 genotyping was performed using a parallel series of PCR reactions, each of which containeUd sequence specific primers designed to amplify different HLA DRB and DQB1 alleles or allele groups (PCR-SSP analysis). In each case the HLA DRB and DQB1 genotypes identified within the decidua and HM were compared. Results-Within the decidual tissue, HLA DRB genotypes were assignable in all nine cases, and HLA DQBl genotypes were identified in seven cases. Within the molar tissue, HLA DRB genotypes were assignable in seven cases, and at least one HLA DQB1 allele was identified in seven cases. Interpretation based on HLA class II genotyping was therefore possible in two cases classified on histological appearances as complete HM, in four classified as partial HM, and in one HM of uncertain type. Different HLA DRB and DQB1 haplotypes were identified within the decidual and molar tissue from both complete HM, consistent with a solely paternal origin and supporting the histological diagnosis. HLA DRB and DQB1 alleles common to the decidual and molar tissue were present within the four partial HM and the HM of histologically uncertain type, consistent with combined maternal and paternal genetic input to these HM, supporting the histological diagnosis in four cases and suggesting that the histologically equivocal case was also a partial HM.Conclusion-PCR-SSP HLA class II DRB and DQB1 typing is reliably applicable to DNA extracted from formalin fixed and paraffin wax embedded tissue. Therefore, in a suitably equipped HLA typing laboratory, this technique provides a useful adjunct to histological examination for differentiation of complete from partial HM. (7 Clin Pathol 1997;50:288-293)

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mentioning

confidence: 99%

Genetic analysis of hydatidiform moles in paraffin wax embedded tissue using rapid, sequence specific PCR-based HLA class II typing.

Bateman

Hemmatpour²,

Theaker³

et al. 1997

Journal of Clinical Pathology

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Genetic analysis of hydratidiform moles utilizing the oligonucleotide-DNA typing of the HLA-DRB gene

Cited by 1 publication

References 34 publications

Genetic analysis of hydatidiform moles in paraffin wax embedded tissue using rapid, sequence specific PCR-based HLA class II typing.

Genetic analysis of hydatidiform moles in paraffin wax embedded tissue using rapid, sequence specific PCR-based HLA class II typing.

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