Genetic Analysis of Field Isolates of Infectious Bursal Disease Virus in Iraqi Farms

Abstract

Sixty samples of bursa of Fabricius were collected from broiler chickens suspected to be infected with infectious bursal disease virus (IBDV) in different areas of Iraq. The extracted nucleic acid was amplified using reverse transcriptase polymerase chain reaction (RT-PCR) targeting genes of segment A (Vp2, Vp3, Vp4 and Vp5 genes) and segment B (VP1 genes). The products of amplification were sent to Korea for sequencing using Sanger method. The sequencing analysis of the IBDV from the Iraqi isolates revealed that each gene had different transition and transversion (nonsense and missense of point mutation) compared to reference genes. The phylogenetic tree analysis showed that the VP2 of segment A of the Iraqi samples was similar to that of an Egyptian strain with 96%similarity, the polypeptide VP2-3-4 of segment A of the Iraqi samples were similar to those of a Chinese strain 99%similarity and the VP5 of segment A was similar to that of Chinese strain with 99% similarity. However, the phylogenetic tree analysis showed that the VP1 of segment B had 95% similarity with that of a Chinese strain.

Evaluation of secondary metabolites of herbal plant extracts as an antiviral effect on infectious bursal disease virus isolates in embryonated chicken eggs

“…Therefore, these results agreed with Cheggag et al [49], who reported that this technique could detect highly virulent IBDV strains from classic and variant IBDV strains. These results also agree with Jumaa et al [50], who confirmed the VP2 of Iraqi vvIBDV strain using sequence analysis.…”

“…These results also agree with Jumaa et al . [ 50 ], who confirmed the VP2 of Iraqi vvIBDV strain using sequence analysis.…”

Protection Activity of T Cell Lymphokines Against Infectious Bursal Disease in Layer Pullets

“…Birds from groups G3 and G2 had the highest prevalence of clinical symptoms and the highest rates of morbidity (both 100%), whereas birds from group G1 had the lowest incidence of clinical signs and the lowest rates of death (both 40 and 8%, respectively). The first group showed lower rates of compare the rates of morbidity and mortality to G2 and G3, which is consistent with Jumaa et al (11) who recorded that finding of cell-mediated immunity, though also linked to the immunopathogenesis of the virus, is crucial for the removal of IBDV and recovery. Rauf et al…”

“…Along with renal alterations, there is an increase in gastrointestinal mucous. The surface of the bursa's serosal cavity is covered in a gelatinous, yellowish transudate in cases of severe dehydration, edema, and hyperemia (11,15).…”