Genetic Analysis of F₂ Population Derived from the Cross Between Perilla Frutescens var. Frutescens and var. Crispa

Abstract

In this study, genetic analysis was conducted on the inheritance of qualitative traits, such as leaf color, flower color, seed size, and seed hardness, in the F2 population derived from the cross between var. frutescens and var. crispa. As determined by the separation pattern of each trait in the F2 population, F2 plants with a green color were more common than those with a purple color on the leaf surface, whereas F2 plants with a purple color were more common than those with a green color on the reverse side of the leaf. For flower color, more F2 plants were observed to have a white color. For seed size, more F2 plants were observed to have a seed size of above 2 mm, and for seed hardness, more F2 plants were observed to have characteristics of seed hardness. Our examination of the genetic separation ratios with respect to Mendel's law of genetics revealed that three traits, leaf color (surface and reverse side) and flower color, did not follow the separation ratio of 1:2:1, and the seed size did not follow the segregation ratio of 3:1, but seed hardness was found to follow the separation ratio of 3:1. The results of this study are expected to provide basic information to help understand the genetic segregation of leaf and seed characteristics in the F2 population derived from the cross between var. frutescens and var. crispa, and also to provide useful information for breeding studies to assist the development of varieties of Perilla crop.

Utilization of Novel Perilla SSR Markers to Assess the Genetic Diversity of Native Perilla Germplasm Accessions Collected from South Korea

“…frutescens could be an escaped form from cultivation, or otherwise likely derived from hybrids between accessions of the CWTPC. Although there is still no accurate report on the natural hybridization rate in the Perilla crop and its weedy varieties, recently Lim et al (2019, 2021) [ 17 , 18 ] and Kim et al (2021) [ 19 ] reported artificial hybridization between cultivated var. frutescens and weedy var.…”

“…The accessions of the CWTPC have the same number of chromosomes, 2n = 40 [ 13 ]. Additionally, cross experiments have been carried out by securing mating between accessions of the CWTPC using artificial pollination [ 14 , 15 , 16 , 17 , 18 , 19 ]. Therefore, it is important to understand the genetic variation and phylogenetic relationships between accessions of the CWTPC, and this will provide a rich source of information for making full use of germplasm resources in Perilla crop breeding programs.…”

Genetic variation and association mapping in the F2 population of the Perilla crop (Perilla frutescens L.) using new developed Perilla SSR markers

“…In addition, more than 500 seeds of the F 2 population are produced in F 1 plant. For this reason, genetic analysis of the entire F 2 population is not possible (Lim et al 2019). Therefore, in this study, some lines of the F 2 population were selected based on leaf characteristics such as leaf color (green or purple) and leaf shape (nonwrinkled or wrinkled) (see Fig.…”

“…frutescens and var. crispa can be crossed with each other by arti cial pollination (Honda et al 1990(Honda et al , 1994Lim et al 2019). For example, Perilla frutescens var.…”

“…Their chromosome number is the same, 2n = 40 (Honda et al 1994;Yamane 1950). A cross experiment between the two cultivated types by Nagai (1935), Honda et al (1990Honda et al ( , 1994 and Lim et al (2019) showed that they are cross-fertile with each other by arti cial pollination. Furthermore, studies by Koezuka et al (1985Koezuka et al ( , 1986, Honda et al (1990), and Ohnishi (2001, 2003) failed to distinguish the two cultivated types of Perilla crop because of the existence of intermediate types.…”