Genetic analysis of downy mildew resistance and identification of molecular markers linked to resistance gene Ppa207 on chromosome 2 in cauliflower

Saha, Partha; Ghoshal, Chandrika; Ray, Soham; Saha, Namita Das; Srivastava, Madhulika; Kalia, Pritam; Tomar, B S

doi:10.1007/s10681-020-02696-6

Cited by 12 publications

(12 citation statements)

References 39 publications

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“…It is a soil-borne disease and can infect at any growth stage of the crop starting from cotyledon to curd stage ( Crute and Gordon, 1987 ). Downy mildew is prevalent in many cruciferous crop growing countries ( Farinhó et al, 2007 ; Carlier et al, 2012 ) and causes 50–60% loss in seed production of cole crops ( Saha et al, 2020 ). The characteristics symptoms of downy mildew appear as angular translucid spots in the intervenial spaces of the leaves showing purplish brown on underside and the upper surface looks tan to yellow color ( Figure 2 ).…”

Section: Downy Mildewmentioning

confidence: 99%

“…tronchuda ( Monteirio et al, 2005 ) and three to four dominant complementary genes in broccoli ( Hoser-Krauze et al, 1995 ). Resistance at adult-plant stage in cauliflower is governed by single dominant gene ( Mahajan et al, 1995 ; Verma and Singh, 2018 ; Saha et al, 2020 ) and recessive epistatic gene ( Mahajan et al, 1995 ). In other varieties of B. oleracea also, the genetic control of adult-plant resistance was found to be controlled by a single dominant gene in broccoli ( Natti and Atkin, 1960 ; Barnes, 1968 ; Coelho and Monteiro, 2003b ), cabbage ( Barnes, 1968 ) and in B. oleracea var.…”

Section: Downy Mildewmentioning

confidence: 99%

“…Singh et al (2015) again reported the same RAPD (OPC141186 and OPE141881) and ISSR (ISSR231103) markers to be linked to downy mildew resistance in cauliflower. Very recently, Saha et al (2020) identified a new monogenic dominant downy mildew resistance gene Ppa207 on chromosome 2 of cauliflower. The new resistance gene was mapped at 4.8 cM interval on linkage group 2 (C02) of cauliflower and was flanked by two SSR markers, BoGMS0486 and BoGMS0900 at a distance of 3.6 and 1.2 cM, respectively, from Ppa207 .…”

Section: Downy Mildewmentioning

confidence: 99%

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Molecular Breeding Strategy and Challenges Towards Improvement of Downy Mildew Resistance in Cauliflower (Brassica oleracea var. botrytis L.)

et al. 2021

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Cauliflower (Brassica oleracea var. botrytis L.) is one of the important, nutritious and healthy vegetable crops grown and consumed worldwide. But its production is constrained by several destructive fungal diseases and most importantly, downy mildew leading to severe yield and quality losses. For sustainable cauliflower production, developing resistant varieties/hybrids with durable resistance against broad-spectrum of pathogens is the best strategy for a long term and reliable solution. Identification of novel resistant resources, knowledge of the genetics of resistance, mapping and cloning of resistance QTLs and identification of candidate genes would facilitate molecular breeding for disease resistance in cauliflower. Advent of next-generation sequencing technologies (NGS) and publishing of draft genome sequence of cauliflower has opened the flood gate for new possibilities to develop enormous amount of genomic resources leading to mapping and cloning of resistance QTLs. In cauliflower, several molecular breeding approaches such as QTL mapping, marker-assisted backcrossing, gene pyramiding have been carried out to develop new resistant cultivars. Marker-assisted selection (MAS) would be beneficial in improving the precision in the selection of improved cultivars against multiple pathogens. This comprehensive review emphasizes the fascinating recent advances made in the application of molecular breeding approach for resistance against an important pathogen; Downy Mildew (Hyaloperonospora parasitica) affecting cauliflower and Brassica oleracea crops and highlights the QTLs identified imparting resistance against this pathogen. We have also emphasized the critical research areas as future perspectives to bridge the gap between availability of genomic resources and its utility in identifying resistance genes/QTLs to breed downy mildew resistant cultivars. Additionally, we have also discussed the challenges and the way forward to realize the full potential of molecular breeding for downy mildew resistance by integrating marker technology with conventional breeding in the post-genomics era. All this information will undoubtedly provide new insights to the researchers in formulating future breeding strategies in cauliflower to develop durable resistant cultivars against the major pathogens in general and downy mildew in particular.

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Section: Downy Mildewmentioning

confidence: 99%

Section: Downy Mildewmentioning

confidence: 99%

Section: Downy Mildewmentioning

confidence: 99%

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Molecular Breeding Strategy and Challenges Towards Improvement of Downy Mildew Resistance in Cauliflower (Brassica oleracea var. botrytis L.)

et al. 2021

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“…Fr. (Giovannelli et al, 2002 ) is another important disease of cauliflower, which damages seedlings as well as mature crops (Laemmlen and Mayberry, 1984 ; Verma and Thakur, 1989 ; Singh et al, 2012 ; Verma and Singh, 2018 ; Saha et al, 2020 ). This also damages the crop in the postharvest stage (Lund and Wyatt, 1978 ; McKay et al, 1992 ).…”

Section: Introductionmentioning

confidence: 99%

“…Singh et al ( 2013 ) identified three markers linked to a downy mildew resistant-gene ( Ppa3 ). Very recently, SSR markers linked to the downy mildew resistant-gene were identified (Saha et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Marker-Assisted Pyramiding of Downy Mildew-Resistant Gene Ppa3 and Black Rot-Resistant Gene Xca1bo in Popular Early Cauliflower Variety Pusa Meghna

et al. 2021

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Cauliflower is an important extensively grown cool season vegetable in India. Black rot and downy mildew are major devastating diseases reducing yield and quality of the crop. To tackle these through host plant resistance, a marker-assisted backcross breeding method was followed to pyramid a black rot-resistant gene (Xca1bo) and a downy mildew-resistant gene (Ppa3) from donors BR-161 and BR-2, respectively, into the background of Pusa Meghna cauliflower cultivar. Marker-assisted backcross breeding was followed up to BC2 generation using SCAR marker ScOPO-04833 and SSR marker BoGMS0624 for black rot and downy mildew resistance genes in foreground selection, respectively. In background selection, at each stage of backcrossing, 47 parental polymorphic SSR markers were used. The graphical genotyping of the five two-gene (Xca1boXca1boPpa3Ppa3) homozygous BC2F2 plants showed an average recovery of 85.44% of the Pusa Meghna genome with highest genome recovery of 91.7%. The genome contribution of donor parents (BR-161 and BR-2) was 8.26 with 6.34% of residual heterozygousity. The backcross derived pyramided lines BC2F2:3-7-16 and BC2F2:3-7-33 showed high resistance to both the diseases and exhibited higher yield and vitamin C content as compared with recipient parent Pusa Meghna. It is, therefore, evident from this study that resistant genes can be introgressed successfully into a Pusa Meghna cultivar without any yield penalty, benefitting farmers with reduced input cost and consumers with chemical residue free produce. Besides, the pyramided lines carrying dominant resistant genes can be exploited in a hybridization programme to develop hybrid(s) in cauliflower.

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Genomic Design for Biotic Stress Tolerance in Vegetable Brassicas

Chhapekar

Singh

et al. 2022

Genomic Designing for Biotic Stress Resistant Vegetable Crops

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Genetic analysis of downy mildew resistance and identification of molecular markers linked to resistance gene Ppa207 on chromosome 2 in cauliflower

Cited by 12 publications

References 39 publications

Molecular Breeding Strategy and Challenges Towards Improvement of Downy Mildew Resistance in Cauliflower (Brassica oleracea var. botrytis L.)

Molecular Breeding Strategy and Challenges Towards Improvement of Downy Mildew Resistance in Cauliflower (Brassica oleracea var. botrytis L.)

Marker-Assisted Pyramiding of Downy Mildew-Resistant Gene Ppa3 and Black Rot-Resistant Gene Xca1bo in Popular Early Cauliflower Variety Pusa Meghna

Genomic Design for Biotic Stress Tolerance in Vegetable Brassicas

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