Genetic analysis of actinobacillus pleuropneumoniae, and comparison with Haemophilus spp. Taxon “Minor Group” and Taxon C

Hampson, David J.; Blackall, P. J.; Woodward, J. M.; Lymbery, Alan J.

doi:10.1016/s0934-8840(11)80494-9

Cited by 20 publications

(9 citation statements)

References 18 publications

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“…Overall, our study found the isolates of serovar 1 were the most diverse (61% diversity), followed by serovar 7 (54% diversity), serovar 15 (44% diversity) and finally serovar 5 (38% diversity). In contrast, a much earlier study from 1993 on genetic diversity within Australian A. pleuropneumoniae, using multilocus enzyme electrophoresis, found no diversity within serovars 1 and 5, and only some diversity in serovars 7 and 12 . That study used a very different assay to assess diversity, compared with the current study, and occurred some time ago, so it is possible that the different results simply reflect methodological and temporal differences.…”

Section: Discussioncontrasting

confidence: 81%

“…In contrast, a much earlier study from 1993 on genetic diversity within Australian A. pleuropneumoniae, using multilocus enzyme electrophoresis, found no diversity within serovars 1 and 5, and only some diversity in serovars 7 and 12 . That study used a very different assay to assess diversity, compared with the current study, and occurred some time ago, so it is possible that the different results simply reflect methodological and temporal differences. A more recent study that used ERIC‐PCR to study 27 serovar 5 isolates obtained from 2002 to 2013 and originating from 11 farms in three Australian states (one of these isolates was used in the present study) also reported little variation .…”

Section: Discussionmentioning

confidence: 99%

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Genetic diversity and toxin gene distribution among serovars of Actinobacillus pleuropneumoniae from Australian pigs

Yee

Blackall

2018

Aust Veterinary J

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Objective To explore the diversity among isolates of the Actinobacillus pleuropneumoniae serovars most common in Australia (serovars 1, 5, 7 and 15) and to examine the Apx toxin profiles in selected representative isolates.Design A total of 250 isolates selected from different farms were examined for their genotypic profiles and a subset of 122 isolates for their toxin profiles.Methods The isolates of serovars 1, 5, 7 and 15 selected for this study came from different farms and different Australian states and were submitted for serotyping to the reference laboratory. The overall diversity of the strains was explored with the enterobacterial repetitive intergenic consensus (ERIC) PCR and the presence of the toxin genes was investigated with a toxin PCR assay.Results Some degree of variation was observed in the ERIC-PCR pattern within all four serovars, ranging from 38% to 61% genetic diversity. When looking at the toxin gene profile and, therefore, the predicted ability to produce the expected toxin pattern, one isolate each of serovars 1 (n = 20) and 7 (n = 47) and 17 isolates of serovar 15 (n = 40) showed variation to the expected gene profile. ConclusionThe variations in toxin gene patterns, as detected by PCR, found in this study could be related to significant changes in the gene sequence or total absence of the gene. Variation in toxin gene sequences has been observed in other countries. This variation in the toxin profile could also explain possible variation in pathogenicity observed in the field.Keywords Actinobacillus pleuropneumoniae; Apx toxin; apx genes; Australia; pigs Abbreviations ERIC, enterobacterial repetitive intergenic consensus; UV, ultraviolet A ctinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia and the virulence of the organism is associated with the exotoxins ApxI, ApxII and ApxIII.

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Section: Discussioncontrasting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Genetic diversity and toxin gene distribution among serovars of Actinobacillus pleuropneumoniae from Australian pigs

Yee

Blackall

2018

Aust Veterinary J

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“…The Porcilis APP vaccine contains 2 of the major virulence factors, ApxI and ApxII, known to be present in serovar 1 isolates of A pleuropneumoniae, as well as a common immunogenic protein, the 42 kDa OMP. As serovar 1 of A pleuropneumoniae is known to be clonal, 30 the antigenic make-up of the live vaccine strain must be very similar to the Australian serovar 1 challenge strain HS54.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the efficacy of a subunit and a live streptomycin‐dependent porcine pleuropneumonia vaccine

Tumamao¹,

Bowles²,

Bosch³

et al. 2004

Aust Veterinary J

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Both of the vaccines provided significant protection against a severe challenge with serovar 1 A pleuropneumoniae. Neither vaccine was effective against a serovar 15 A pleuropneumoniae challenge. There was evidence that the Porcilis APP vaccine did provide some protection against the serovar 15 challenge because the ADG, after challenge of pigs given this vaccine, was greater than the control pigs.

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“…The ability to discriminate between strains of bacterial pathogens, such as A. pleuropneumoniae, is advantageous in better understanding disease transmission, tracking virulent or antibiotic-resistant strains, and probing the nature of evolutionary biology (46). In the case of A. pleuropneumoniae, a number of different techniques have been used to discriminate between strains, including multilocus enzyme electrophoresis (14,30,31), HindIII ribotyping (11,12,16), PCR-restriction endonuclease analysis (16,50) or PCR-restriction fragment length polymorphism (PCR-RFLP) (6, 7), ApxI-III toxin typing alone (2,10) or in combination with outer membrane lipoprotein A (omlA)-typing (13), and ApxI-IV toxin typing (39,47). However, the gold standard method remains serotyping, to which all of the newer techniques are compared, but with which there is a correlation to differing extents.…”

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confidence: 99%

Multiplex PCR That Can Distinguish between Immunologically Cross- Reactive Serovar 3, 6, and 8 Actinobacillus pleuropneumoniae Strains

et al. 2008

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Genetic analysis of actinobacillus pleuropneumoniae, and comparison with Haemophilus spp. Taxon “Minor Group” and Taxon C

Cited by 20 publications

References 18 publications

Genetic diversity and toxin gene distribution among serovars of Actinobacillus pleuropneumoniae from Australian pigs

Genetic diversity and toxin gene distribution among serovars of Actinobacillus pleuropneumoniae from Australian pigs

Comparison of the efficacy of a subunit and a live streptomycin‐dependent porcine pleuropneumonia vaccine

Multiplex PCR That Can Distinguish between Immunologically Cross- Reactive Serovar 3, 6, and 8 Actinobacillus pleuropneumoniae Strains

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