Genetic analysis and identification of two soybean mosaic virus resistance genes in soybean [Glycine max (L.) Merr]

Abstract

Soybean mosaic virus (SMV) commonly affects soybean production worldwide, and the SC18 strain has been widespread in China. This study aimed to characterize and map the SC18 resistance genes present in soybean cultivars 'Kefeng No.

Characterization of broad‐spectrum resistance to Soybean mosaic virus in soybean [Glycine max (L.) Merr.] cultivar ‘RN‐9’

“…Genetic linkage maps of SMV resistance loci for SC10, SC14, SC15 and SC18; (a) the linkage map of soybean linkage group C2 (chromosome 6) (Song et al. ); (b) the integrated linkage map of Rsc14‐r , Rsc15 , Rsc10‐r and Rsc18‐r with the RIL population from RN‐9 × 7605; (c) the linkage map of the resistance gene for SC14 in Qihuang No.1 (Ma et al., ); (d) the linkage map of the resistance gene for SC10 in Kefeng No.1 (Li et al., ); (e and f) the linkage maps of the resistance genes for SC18 in Kefeng No.1 and Qihuang 22, respectively (Li et al., ) [Colour figure can be viewed at wileyonlinelibrary.com]…”

“…Though each of soybean cultivars KF, QH, ‘Qihuang 22’ and ‘RN‐9’ possesses a dominant gene underlying resistance to SMV strain SC18 (Li et al., , ; Rui et al., ), allelism relationships among these genes have not yet been fully investigated. Li et al.…”

“…Soybean mosaic virus strains (SC1‐SC21) used in this study were provided by the National Center for Soybean Improvement (NCSI, Nanjing, China) and maintained in leaves of susceptible cultivar ‘Nannong 1138‐2’ (NN). SMV strains SC10, SC14, SC15 and SC18 were identical with those previously used (Li et al., , ; Ma et al., ; Rui et al., ; Yang & Gai, ). The SMV inoculation and serological determination (double antibody sandwich enzyme‐linked immunosorbent assay, DAS‐ELISA) was conducted (Gao et al., ; Li et al., ) following the manufacturer's instructions (ACD Inc., Fayetteville, AR, USA).…”

“…Linkage analysis of genes underling soybean resistance to the four SMV strains in ‘RN‐9’ was conducted using the RIL population. Combining the phenotypic data in the present study and previous genotypic data of the RIL population (Li et al., ; Rui et al., ), integrated linkage map of SMV resistance gene(s) was constructed as Rui et al. () described.…”

“…In China, genes conferring resistance to multiple SMV strains in ‘Kefeng No.1’ (KF) were clustered in one region on chromosome 2 (linkage group, LG‐D1b). (Fu, Zhan, Zhi, Gai, & Yu, ; Li et al., , ; Wang, Ma, Yang et al., ; Wang, Zhi, Guo et al., 2004); SMV resistance genes identified from ‘Qihuang No.1’ (QH) and ‘Qihuang 22’ were mapped on chromosome 13 (LG‐F) (Li et al., ; Ma, Li, Wang, Liu, & Zhi, ; Ma et al., ; Zheng et al., ); strain‐specific resistance genes in ‘RN‐9’ ( Rsc15 ) and Dabaima ( Rsc4 ) were identified on chromosome 6 (LG‐C2) and 14 (LG‐B2), respectively (Rui et al., ; Wang, Ma, Liu et al., ; Yang & Gai, ). In addition, several major quantitative trait loci (QTLs) conferring soybean resistance to SMV have been detected on chromosome 6, 10 and 13 etc.…”