Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.)

Shí, Yùzhēn; Chen, Jie; Liu, WenQiang; Huang, Qi-na; Bo, Shen; Leung, Hei; Wu, Jianli

doi:10.1007/s11427-009-0109-1

Cited by 23 publications

(16 citation statements)

References 18 publications

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“…The primers were synthesized by Sangon Biotech (Shanghai, China). PCR reaction and detection were carried out as described previously (Shi et al ).…”

Section: Methodsmentioning

confidence: 99%

Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice

Huang

Shí

Zhang

et al. 2015

JIPB

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A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate‐induced rice IR64 mutant bank. The premature senescence phenotype appeared at the six‐leaf stage and the plant died at the early heading stage. psd128 exhibited impaired chloroplast development with significantly reduced photosynthetic ability, chlorophyll and carotenoid contents, root vigor, soluble protein content and increased malonaldehyde content. Furthermore, the expression of senescence‐related genes was significantly altered in psd128. The mutant trait was controlled by a single recessive nuclear gene. Using map‐based strategy, the mutation Oryza sativa cell division cycle 48 (OsCDC48) was isolated and predicted to encode a putative AAA‐type ATPase with 809 amino‐acid residuals. A single base substitution at position C2347T in psd128 resulted in a premature stop codon. Functional complementation could rescue the mutant phenotype. In addition, RNA interference resulted in the premature senescence and death phenotype. OsCDC48 was expressed constitutively in the root, stem, leaf and panicle. Subcellular analysis indicated that OsCDC48:YFP fusion proteins were located both in the cytoplasm and nucleus. OsCDC48 was highly conserved with more than 90% identity in the protein levels among plant species. Our results indicated that the impaired function of OsCDC48 was responsible for the premature senescence and death phenotype.

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“…The primers were synthesized by Sangon Biotech (Shanghai, China). PCR reaction and detection were carried out as described previously (Shi et al ).…”

Section: Methodsmentioning

confidence: 99%

Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice

Huang

Shí

Zhang

et al. 2015

JIPB

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show abstract

“…Other important traits in rice breeding programs induced by mutagenesis include early flowering, low phytic acid content and giant embryo size [12]. The IR64 mutant library itself has been a source of resistance to diseases [13] [14] [15], high biomass production [Barroiden M, IRRI, personal communication], salt tolerance [16] and drought tolerance [17], as well as the identification of genes involved in leaf development [18].…”

Section: Introductionmentioning

confidence: 99%

Increasing Leaf Vein Density by Mutagenesis: Laying the Foundations for C4 Rice

et al. 2014

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A high leaf vein density is both an essential feature of C4 photosynthesis and a foundation trait to C4 evolution, ensuring the optimal proportion and proximity of mesophyll and bundle sheath cells for permitting the rapid exchange of photosynthates. Two rice mutant populations, a deletion mutant library with a cv. IR64 background (12,470 lines) and a T-DNA insertion mutant library with a cv. Tainung 67 background (10,830 lines), were screened for increases in vein density. A high throughput method with handheld microscopes was developed and its accuracy was supported by more rigorous microscopy analysis. Eight lines with significantly increased leaf vein densities were identified to be used as genetic stock for the global C4 Rice Consortium. The candidate population was shown to include both shared and independent mutations and so more than one gene controlled the high vein density phenotype. The high vein density trait was found to be linked to a narrow leaf width trait but the linkage was incomplete. The more genetically robust narrow leaf width trait was proposed to be used as a reliable phenotypic marker for finding high vein density variants in rice in future screens.

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“…Therefore, moderate leaf rolling is an ideal trait for rice breeding (Price et al, 1997). To date, 12 rice mutants with rolled leaves (rl) have been isolated and reported in several different studies, for which six genes (rl1-rl6) were mapped on corresponding rice chromosomes through conventional genetic screening, and rl7 to rl12 were mapped to chromosomes 5 (rl7 and rl8; Li et al, 2000), 7 (rl11; Shi et al, 2009), 9 (rl9 [Yan et al, 2006] and rl10 [Luo et al, 2007]), and 10 (rl12; Luo et al, 2009). However, only a few mutant genes have been cloned and characterized, and most of these leaf-rolling genes are associated with leaf adaxial-abaxial polarity establishment.…”

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confidence: 99%

Leaf Rolling Controlled by the Homeodomain Leucine Zipper Class IV GeneRoc5in Rice

et al. 2011

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Leaf rolling is considered an important agronomic trait in rice (Oryza sativa) breeding. To understand the molecular mechanism controlling leaf rolling, we screened a rice T-DNA insertion population and isolated the outcurved leaf1 (oul1) mutant showing abaxial leaf rolling. The phenotypes were caused by knockout of Rice outermost cell-specific gene5 (Roc5), an ortholog of the Arabidopsis (Arabidopsis thaliana) homeodomain leucine zipper class IV gene GLABRA2. Interestingly, overexpression of Roc5 led to adaxially rolled leaves, whereas cosuppression of Roc5 resulted in abaxial leaf rolling. Bulliform cell number and size increased in oul1 and Roc5 cosuppression plants but were reduced in Roc5-overexpressing lines. The data indicate that Roc5 negatively regulates bulliform cell fate and development. Gene expression profiling, quantitative polymerase chain reaction, and RNA interference (RNAi) analyses revealed that Protodermal Factor Like (PFL) was probably down-regulated in oul1. The mRNA level of PFL was increased in Roc5-overexpressing lines, and PFL-RNAi transgenic plants exhibit reversely rolling leaves by reason of increases of bulliform cell number and size, indicating that Roc5 may have a conserved function. These are, to our knowledge, the first functional data for a gene encoding a homeodomain leucine zipper class IV transcriptional factor in rice that modulates leaf rolling.

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Genetic analysis and gene mapping of a new rolled-leaf mutant in rice (Oryza sativa L.)

Cited by 23 publications

References 18 publications

Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice

Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice

Increasing Leaf Vein Density by Mutagenesis: Laying the Foundations for C4 Rice

Leaf Rolling Controlled by the Homeodomain Leucine Zipper Class IV GeneRoc5in Rice

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