Genetic analysis and gene mapping of a dominant presenescing leaf gene PSL3 in rice (Oryza sativa L.)

Fang, Likui; Li, Yunfeng; Gong, Xiaoping; Sang, Xianchun; Ling, Yinghua; Wang, Xiaowen; Cong, YunFei; He, Guanghua

doi:10.1007/s11434-010-4013-7

Cited by 17 publications

(23 citation statements)

References 29 publications

(25 reference statements)

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“…When their activities are reduced, the ROS concentration increases and membrane lipid peroxidation is enhanced (Leshem et al, 1981). The mutants psl1, es-t, sms1, psl3, and esl3 each display a senescent phenotype as early as the seedling stage, and the genes responsible for these phenotypes have been mapped to chromosomes 2, 5, 8, 7, and 5, respectively (Fang et al, 2010;Miao et al, 2013;Wang et al, 2006;Yan et al, 2010;Yang et al, 2011 ). The accelerated and accentuated leaf senescence observed in esl4 might be associated with disruption of the system that regulates the abundances of ROS.…”

Section: Discussionmentioning

confidence: 99%

“…This suggests that a novel mutation is responsible for early senescence in esl4. The mutants psl1, es-t, sms1, psl3, and esl3 each display a senescent phenotype as early as the seedling stage, and the genes responsible for these phenotypes have been mapped to chromosomes 2, 5, 8, 7, and 5, respectively (Fang et al, 2010;Miao et al, 2013;Wang et al, 2006;Yan et al, 2010;Yang et al, 2011 ). The psl2 and lad mutants show yellowing of leaves at the late tillering stage, and the mutant genes responsible for these phenotypes have been mapped to chromosomes 8 and 11, respectively (Du et al, 2012;Zhang et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The leaf cellular structure of the wild-type and esl4 were observed by transmission electron microscopy (TEM), as described by Fang et al (2010). The apex and bottom of the flag leaf were fixed in primary fixative solution (2.5% glutaraldehyde) and then post-fixed for 2 h with 1% osmium tetroxide after washing with 0.1 mol L -1 phosphate-buffered saline.…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

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Identification and Gene Mapping of anearly senescence leaf 4Mutant of Rice

et al. 2014

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2713ReseaRch T he leaf is the primary site of photosynthesis and respiration in plants. The shape and lifespan of the leaf is strongly associated with crop yield (Yan et al., 2008). Early senescence often causes significant losses in crop yield and quality. For example, delay of leaf senescence by only 1 d theoretically increases rice grain yield by 2%, with an approximately 1% increase in grain yield observed in practice (Duan et al., 1997). Therefore, studying premature senescence genes in rice is crucial not only for molecular breeding of rice, but also for comparing the mechanisms responsible for leaf senescence in monocotyledonous and dicotyledonous plants. Leaf senescence is a complex physiological event that constitutes the last stage of leaf development and aBSTRaCT An early senescence leaf 4 (esl4) mutant of rice (Oryza sativa L.) displayed yellowing followed by severe chlorosis of the leaf apex and margin from the late jointing stage to maturity. The content of photosynthetic pigments in the mutant was significantly lower than in wild-type plants, and abnormalities in cell structure were detected in the mutant leaf blade. Stomatal conductance and rates of photosynthesis and transpiration were lower in esl4 than in wild-type plants. Furthermore, compared with the wild-type, the activities of superoxide dismutase and catalase were significantly lower in esl4, whereas peroxidase activity was significantly higher in esl4. Genetic analysis indicated that the early senescence of esl4 was controlled by a recessive nuclear gene. A population of 879 F 2 mutants was used to fine-map the ESL4 gene to chromosome 4 between the insertion and deletion primers S4-42 and S4-67. This 63-kb stretch encodes 12 uncharacterized open reading frames. Quantitative real-time polymerase chain reaction analysis (qrT-pCr) revealed that levels of SGR transcripts were considerably more abundant in esl4 than in wild-type. These results provide a foundation for map-based cloning and functional analysis of the ESL4 gene.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Transmission Electron Microscopymentioning

confidence: 99%

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Identification and Gene Mapping of anearly senescence leaf 4Mutant of Rice

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“…These included psl1 (Wang et al, 2006), pgl2 (Zhu et al, 2007), psl3 (Fang et al, 2010), esl2 (Xu et al, 2012), lad (Du et al, 2012), esl3 (Miao et al, 2013), esl4 (Guo et al, 2014), esl5 , esl6 (Yang et al, 2016a), esl9 (Xiao et al, 2017), and esl10 (Chen et al, 2018). These included psl1 (Wang et al, 2006), pgl2 (Zhu et al, 2007), psl3 (Fang et al, 2010), esl2 (Xu et al, 2012), lad (Du et al, 2012), esl3 (Miao et al, 2013), esl4 (Guo et al, 2014), esl5 , esl6 (Yang et al, 2016a), esl9 (Xiao et al, 2017), and esl10 (Chen et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Numerous early leaf senescence mutants of rice have been identified, and their molecular positioning has been reported. These included psl1 (Wang et al, 2006), pgl2 (Zhu et al, 2007), psl3 (Fang et al, 2010), esl2 (Xu et al, 2012), lad (Du et al, 2012), esl3 (Miao et al, 2013), esl4 (Guo et al, 2014), esl5 , esl6 (Yang et al, 2016a), esl9 (Xiao et al, 2017), and esl10 (Chen et al, 2018). However, few senescence-associated genes have been cloned in rice, such as NYC4 (Yamatani et al, 2013), OsNAP (Liang and Chu, 2015), NOE1 (Lin et al, 2012), and LTN1 (Hu et al, 2011).…”

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Identification and Gene Mapping of an Early Senescent Leaf Mutant esl11 of Rice

Wang

Xie

et al. 2018

Crop Science

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As an essential process in the life of a plant, natural leaf senescence is necessary for adaptation to environmental diversity, whereas early leaf senescence can reduce per‐unit yield and cause inferior harvest quality during crop production. To explore the molecular mechanism of leaf senescence in rice (Oryza sativa L.), we studied an early senescence leaf (esl11) mutant obtained by ethyl methane sulfonate (EMS) mutagenesis. The leaves of the esl11 mutant showed normal growth and leaf coloration prior to the three‐leaf stage, but after this stage, all fully expanded leaves underwent yellowing and senescence from the edges of the leaf blade and tips, apart from the emerging leaves, which showed gradual growth. The net photosynthetic rate and photosynthetic pigment content of the tip and leaf base of the mutant esl11 were significantly lower than those of the wild type. The role of active oxygen species O2−, H2O2, and ⋅OH in the mutant increased, whereas the activities of MDA, peroxidase, superoxide dismutase, and catalase decreased compared with the wild type. The results of trypan blue staining on the senescent part of the leaf tip of the mutant esl11 indicated procedural death in part of the cell, whereas the wild type showed no change. The early leaf senescence trait in the mutant was controlled by of recessive nuclear gene, which was mapped to chromosome 7, with a physical range of 143.0 kb. The results of this study provide the foundation for important progress in gene cloning and functional analysis of the esl11 gene.

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Effect of nitrogen rates on the growth and grain yield of a leaf early‐senescent rice mutant

Zhu

et al. 2021

Agronomy Journal

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Nitrogen plays a vital role in regulating leaf senescence and yield formation in rice (Oryza sativa L.). However, little information is known about the effects of N rates on the growth and grain yield of rice plants with leaf early senescence. This study used a leaf early-senescent rice mutant (osled) and its wild type (WT) (93-11) and investigated how N rates of 0 kg ha -1 (0N), 240 kg ha -1 (240N), and 360 kg ha -1 (360N) influenced the main agronomic and physiological performance of the mutant osled. The results showed that 240N and 360N treatments increased grain yield of the osled plants by 201.5-222.9% and 266.4-287.9% as compared with 0N treatment, respectively, with the 360N treatment maintaining a similar agronomic nitrogen use efficiency (ANUE) as 240N. Leaf activity of the osled plants was enhanced with N supply levels showing increased leaf area, chlorophyll content, N content, and activities of N-related enzymes and photosynthetic rate, and lowered malondialdehyde content. In contrast to 0N, the two N treatments significantly increased plant height, aboveground biomass, tiller number and percentage of productive tillers of both rice genotypes. Compared with 240N, the 360N treatment particularly increased grain weight and percentage of filled grains for the osled plants through improving leaf activity, root activity, and grain activity. These results verify that N treatment regulates leaf senescence and reduces yield loss of the osled plants, and simultaneously implied that the osled plants more positively respond to high N supply.

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Genetic analysis and gene mapping of a dominant presenescing leaf gene PSL3 in rice (Oryza sativa L.)

Cited by 17 publications

References 29 publications

Identification and Gene Mapping of anearly senescence leaf 4Mutant of Rice

Identification and Gene Mapping of anearly senescence leaf 4Mutant of Rice

Identification and Gene Mapping of an Early Senescent Leaf Mutant esl11 of Rice

Effect of nitrogen rates on the growth and grain yield of a leaf early‐senescent rice mutant

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