Genetic analysis and gene fine mapping for a rice novel mutant (rl 9(t) ) with rolling leaf character

Abstract

Leaf shape is an important parameter for ideotype breeding in rice, and the rolling of leaf is also beneficial to efficient ripening of grains. This encourages the explorations of new genes that regulate leaf shape. In this study, genetic analysis and gene mapping were carried out for a novel rolling leaf mutant identified from japonica variety Zhonghua 11. The SSR marker analysis showed that the mutant was controlled by a single recessive gene (rl 9(t) ) located on chromosome 9. Fine mapping of the Rl 9(t) locus was conducted with 30 new STS markers developed around Rl 9(t) anchored region based on the sequence diversity between Nipponbare and 93-11. The fine mapping necessitated the contruction of a PAC contig encompassing the Rl 9(t) locus, which was delimited to a 42 kb region. This could therefore enhance the cloning of the target gene in further studies.

SHALLOT-LIKE1 Is a KANADI Transcription Factor That Modulates Rice Leaf Rolling by Regulating Leaf Abaxial Cell Development

“…Up to now, 11 rice mutants with rolled leaves (rl) have been characterized, for which six recessive genes (rl1-rl6) were mapped on rice chromosomes 1, 4, 12, 3, or 7 by morphological markers (Kinoshita, 1984;Khush and Kinoshita, 1991; http://www.gramene.org/). rl7-rl10 were mapped to chromosomes 5 (rl7; Li et al, 1998;rl8, 542 kb;Shao et al, 2005a) and 9 (rl9, 42 kb; Yan et al, 2006;rl10, 194 kb;Luo et al, 2007) by molecular markers. Rl(t), an incomplete recessive rolled leaf mutant, was fine-mapped to a 137-kb region of the long arm of chromosome 2 (Shao et al, 2005b).…”

Leaf Rolling Controlled by the Homeodomain Leucine Zipper Class IV GeneRoc5in Rice

“…Therefore, moderate leaf rolling is an ideal trait for rice breeding (Price et al, 1997). To date, 12 rice mutants with rolled leaves (rl) have been isolated and reported in several different studies, for which six genes (rl1-rl6) were mapped on corresponding rice chromosomes through conventional genetic screening, and rl7 to rl12 were mapped to chromosomes 5 (rl7 and rl8; Li et al, 2000), 7 (rl11; Shi et al, 2009), 9 (rl9 [Yan et al, 2006] and rl10 [Luo et al, 2007]), and 10 (rl12; Luo et al, 2009). However, only a few mutant genes have been cloned and characterized, and most of these leaf-rolling genes are associated with leaf adaxial-abaxial polarity establishment.…”

Functional Analysis for Rolling Leaf of Somaclonal Mutants in Rice (Oryza sativa L.)

“…Also the rl1~rl6 alelle, recessive gene of 6 kinds, were situated within each chromosome 1, 4, 12, 3, 7 thru formal marker analysis [12,13]. The rl7~rl10 alelle mapping in the chromosome 5 [16,17,21,26,27], Rl(t), had an incomplete recessive gene, mapping between the long arms (137 kb area) of chromosome 2 [22]. Yet there was also no report that there was any direct gene cloning.…”