Genetic analysis and fine mapping of the gall midge resistance gene Gm5 in rice (Oryza sativa L.)

Zhou, Huanjiao Jenny; Wang, Xinyi; Mo, Yufei; Yang, Li; Yan, Liuhui; Li, Zhihua; Shu, Wan; Ling, Cheng; Huang, Fang; Qiu, Yongfu

doi:10.1007/s00122-020-03575-3

Cited by 10 publications

(13 citation statements)

References 34 publications

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“…Significant progress has also been made in identifying, tagging and pyramiding the genes conferring resistance to different rice gall midge biotypes using marker assisted selection to develop midge-resistant rice varieties 43 . Similarly, the avirulent and virulent response of different Hessian fly, Mayetiola destructor (Say) populations in USA in wheat cultivars or germplasm lines have led to identification of several biotypes and the resistance genes to develop Hessian fly-resistant wheat varieties 44,45 .…”

Section: Discussionmentioning

confidence: 99%

Biological and biochemical diversity in different biotypes of spotted stem borer, Chilo partellus (Swinhoe) in India

Dhillon

Tanwar

Kumar

et al. 2021

Sci Rep

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Because of variation in incidence and severity of damage by Chilo partellus (Swinhoe) in different geographical regions, it is difficult to identify stable sources of resistance against this pest. Therefore, the present studies were undertaken on biological attributes (damage in resistant and susceptible genotypes, survival and development) and biochemical profiles (amino acids and lipophilic compound) of C. partellus populations from eight geographical regions to understand it’s population structure in India. There was a significant variation in biological attributes and biochemical profiles of C. partellus populations from different geographical regions. Based on virulence and biological attributes, similarity index placed the C. partellus populations in five groups. Likewise, lipophilic and amino acid profiling also placed the C. partellus populations in five groups. However, the different clusters based on biological and biochemical attributes did not include populations from the same regions. Similarity index based on virulence, biological attributes, and amino acids and lipophilic profiles placed the C. partellus populations in six groups. The C. partellus populations from Hisar, Hyderabad, Parbhani and Coimbatore were distinct from each other, indicating that there are four biotypes of C. partellus in India. The results suggested that sorghum and maize genotypes need to be tested against these four populations to identify stable sources of resistance. However, there is a need for further studies to establish the restriction in gene flow through molecular approaches across geographical regions to establish the distinctiveness of different biotypes of C. partellus in India.

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Section: Discussionmentioning

confidence: 99%

Biological and biochemical diversity in different biotypes of spotted stem borer, Chilo partellus (Swinhoe) in India

Dhillon

Tanwar

Kumar

et al. 2021

Sci Rep

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“…The varieties Kangwenqingzhan (abbreviated KW) and ARC5984 are highly resistant to RGM insects, whereas rice line 9311 is susceptible to RGM. KW and ARC5984 have been detected to carry the RGM resistance genes Gm6 and Gm5 , respectively [ 9 , 13 ]. Backcrossing generations and near-isogenic lines (NILs) with a 9311 genetic background were developed according to the method described by Li et al [ 9 ] and Zhou et al [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…KW and ARC5984 have been detected to carry the RGM resistance genes Gm6 and Gm5 , respectively [ 9 , 13 ]. Backcrossing generations and near-isogenic lines (NILs) with a 9311 genetic background were developed according to the method described by Li et al [ 9 ] and Zhou et al [ 13 ]. Specifically, marker YW21 was applied to detect the heterozygosity at the Gm6 region of BC 2 F 1 individuals, which were self-pollinated to develop the BC 2 F 2 population.…”

Section: Methodsmentioning

confidence: 99%

“…The damage level of rice plants against RGM was divided into six grades as described by IRRI: 0, free of damage; < 5%, highly resistant; 6%–10%, resistant; 11%–20%, moderately resistant; 21%–50% susceptible; and > 50%, highly susceptible (Table 1 ). Seedlings with inflated shoots or multiple tillers were considered resistant as the larvae could not damage the stem tip continuously and finally died, according to observations from our previous studies [ 9 , 13 ]. Therefore, the evaluation criterion was simplified to four grades as follows: < 5%, highly resistant; 6–15%, resistant; 16–50%, susceptible; and > 50%, highly susceptible (Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

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Improved phenotyping procedure for evaluating resistance in rice against gall midge (Orseolia oryzae, Wood-Mason)

et al. 2021

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Background The rice gall midge (RGM, Orseolia oryzae, Wood-Mason), an important stem-feeding pest worldwide, has caused serious production losses over the past decades. Rice production practices indicate that the most reliable method for managing RGM is the deployment of cultivars that incorporate host resistance. However, the conventional phenotypic screening method of rice resistance to RGM suggested by the International Rice Research Institute (IRRI) has been used for approximately 30 years, and only 12 rice varieties/lines (including controls) can be evaluated in one tray. It is not suitable for high-throughput phenotyping of rice germplasm. Moreover, a suitable method to prepare samples for molecular biological studies of rice resistance against RGM is imperative with the rapid development of modern molecular techniques. Results The proper density of seedlings/RGM was determined for four seeding arrangements. A high-throughput phenotyping method (HTPM) for 60 lines/varieties infested with 36 female RGM adults in one tray, as described by method 4–3 (seeded 60 lines/varieties), was developed and verified using mutant screening. Furthermore, one RGM resistance gene flanked by markers 12RM28346 and 12RM28739 on chromosome 12 was simultaneously detected using method 2–2 (seeded 30 lines/varieties in one tray) treated with 24 RGM and analyzed using conventional and simplified grading systems. Genetic analysis of the RGM resistance gene was confirmed using a method identical to that suggested by IRRI. Finally, one bucket with 24 seedlings treated with at least five female RGM adults was efficacious and could offer adequate samples for insect development observation or molecular biological studies. Conclusion A highly efficient and reliable procedure for evaluation of resistance in rice to RGM was developed and improved, and was verified through mutant screening, gene mapping, genetic analysis, and insect growth and development observations.

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“…Rice blast, bacterial blight (BB), brown planthopper (BPH), gall midge (GM) and brown spot (BS) are major biotic constraints in rice. More than 100 major genes and over 350 QTLs for blast resistance (Chen et al ., 2020); more than 44 (dominant and recessive) genes for BB resistance (Kim and Reinke, 2019), more than 11 genes and several QTLs for GM resistance (Zhou et al ., 2020), 31 genes and number of QTLs for BPH resistance (Chen et al ., 2020), and 12 QTLs for BS resistance (Mizobuchi et al ., 2016) are reported. Major QTLs/genes for biotic and abiotic stress and their donors to be used in the DDSR breeding programme are mentioned in Table 1.…”

Section: Traits For Developing Ddsr Varietiesmentioning

confidence: 99%

More and more of less and less: Is genomics‐based breeding of dry direct‐seeded rice (DDSR) varieties the need of hour?

Sagare¹,

Abbai

Jain³

et al. 2020

Plant Biotechnology Journal

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Rice is a staple food for half of the world's population. Changing climatic conditions, water and labour scarcity are the major challenges that shall limit future rice production. Dry direct-seeded rice (DDSR) is emerging as an efficient, resources conserving, mechanized, climate smart and economically viable strategy to be adopted as an alternative to puddled transplanted rice (TPR) with the potential to address the problem of labour-water shortages and ensure sustainable rice cultivation. Despite these benefits, several constraints obstruct the adoption of DDSR. In principle, the plant type for DDSR should be different from one for TPR, which could be achieved by developing rice varieties that combine the traits of upland and lowland varieties. In this context, recent advances in precise phenotyping and NGS-based trait mapping led to identification of promising donors and QTLs/genes for DDSR favourable traits to be employed in genomic breeding. This review discusses the important traits influencing DDSR, research studies to clarify the need for breeding DDSR-specific varieties to achieve enhanced grain yield, climate resilience and nutrition demand. We anticipate that in the coming years, genomic breeding for developing DDSR-specific varieties would be a regular practice and might be further strengthened by combining superior haplotypes regulating important DDSR traits by haplotypebased breeding.

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Genetic analysis and fine mapping of the gall midge resistance gene Gm5 in rice (Oryza sativa L.)

Cited by 10 publications

References 34 publications

Biological and biochemical diversity in different biotypes of spotted stem borer, Chilo partellus (Swinhoe) in India

Biological and biochemical diversity in different biotypes of spotted stem borer, Chilo partellus (Swinhoe) in India

Improved phenotyping procedure for evaluating resistance in rice against gall midge (Orseolia oryzae, Wood-Mason)

More and more of less and less: Is genomics‐based breeding of dry direct‐seeded rice (DDSR) varieties the need of hour?

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