Genetic algorithm-based medium optimization for a toxic dinoflagellate microalga

Camacho, F. Garcı́a; Gallardo, Juan José; Sánchez-Mirón, A.; Chisti, Yusuf; Grima, E. Molina

doi:10.1016/j.hal.2011.05.005

Cited by 15 publications

(16 citation statements)

References 15 publications

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“…The composition in Table 1 is substantially different relative to the composition reported for growing the dinoflagellate P. reticulatum [8] and also determined by a genetic algorithm-based search starting from the same initial array of ingredients as used in the present work.…”

Section: Genetic-algorithm-based Optimizationmentioning

confidence: 75%

“…Urea is a commonly used organic nitrogen source for microalgae [10]. Ammonium chloride was not used as it previously proved toxic to the dinoflagellate P. reticulatum [8,15]. Furthermore, ammonium chloride tends to release the highly toxic ammonia under alkaline conditions and this is harmful to most algae.…”

Section: Genetic-algorithm-based Optimizationmentioning

confidence: 99%

“…As toxin productivity and growth of diverse dinoflagellates are highly influenced by nutrition [7], development of suitable culture media formulations for them becomes a key factor for addressing a dinoflagellate-based bioprocess. The L1 medium that has been traditionally used to grow many dinoflagellates was originally developed for growing diatoms and has been shown to be inadequate for highly productive culture of dinoflagellates [8]. For example, based on the published elemental composition of the marine phycoplanktons, including dinoflagellates [9], the nitrogen (N) and phosphorous (P) contents of the biomass are 11.9% and 1.6% by weight, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…A genetic-algorithm-based approach has been previously shown to be quite successful for formulating a culture medium of the dinoflagellate Protoceratium reticulatum [8,11]. Genetic algorithm (GA) based methods are superior to the conventional statistical experimental designs that have been commonly used for developing microbial culture media.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

An optimal culture medium for growing Karlodinium veneficum : Progress towards a microalgal dinoflagellate-based bioprocess

et al. 2015

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Section: Genetic-algorithm-based Optimizationmentioning

confidence: 75%

Section: Genetic-algorithm-based Optimizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An optimal culture medium for growing Karlodinium veneficum : Progress towards a microalgal dinoflagellate-based bioprocess

et al. 2015

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“…The seawater culture medium used was the commercial ALGAL (Bionova, Santiago de Compostela, Spain) with the following composition: 0. 8 …”

Section: Microorganism and Culture Maintenancementioning

confidence: 99%

Genetic algorithm for the medium optimization of the microalga Nannochloropsis gaditana cultured to aquaculture

Camacho-Rodríguez

Cerón-García

Sevilla

et al. 2015

Bioresource Technology

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High‐throughput evolutionary optimization of the induction medium towards recombinant protein production in BY‐2 tobacco

Sadoch

Pyc

Urbanowicz

et al. 2020

Biotech & Bioengineering

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Bright yellow (BY‐2) tobacco cells combined with the XVE chemically inducible system are one of the most promising plant‐based platforms for recombinant protein production. This offers a range of benefits, including the separation of the cell growth and heterologous gene expression, lack of risk of infecting the end product with prions and human viruses or appropriate protein glycosylation and folding. However, low protein productivity remains a major obstacle that limits the extensive commercialization of bioproduction in plants. A number of molecular, cell culture and down processing approaches have been made to overcome this problem. Media development for the specific nutritional and hormonal requirements of transgenic plant cells is one of the most efficient cell‐culture approaches. We optimized the induction medium towards recombinant protein production in BY‐2 and demonstrated the usefulness of evolutionary medium optimization for high‐yield protein production in liquid plant cultures. A reliable XVE/GFP model, parallel conducting experiments in a microscale on 96‐well plates, and dedicated Gene Game evolutionary optimization software allowed for an effective search of 7611 possible solutions of 11‐component media. Within the 4608 formulations tested, the Induct X medium was found with a significant 107.14% increase in protein expression in relation to the standard BY‐2 medium.

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Genetic algorithm-based medium optimization for a toxic dinoflagellate microalga

Cited by 15 publications

References 15 publications

An optimal culture medium for growing Karlodinium veneficum : Progress towards a microalgal dinoflagellate-based bioprocess

An optimal culture medium for growing Karlodinium veneficum : Progress towards a microalgal dinoflagellate-based bioprocess

Genetic algorithm for the medium optimization of the microalga Nannochloropsis gaditana cultured to aquaculture

High‐throughput evolutionary optimization of the induction medium towards recombinant protein production in BY‐2 tobacco

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