Genesis of the 14q+ marker in Burkitt's lymphoma

Abstract

By means of the “prosome” analysis technique, by which chromosomes are studied at early metaphase and even at prophase, an improved resolution of the G‐band pattern has been achieved. The 2 chromosome regions of especial significance in the (8; 14) translocation of Burkitt's lymphoma, 8q24 and 14q32, which in the Pans karyotype were represented as uniformly negative, in prosome analysis resolved into 5 and 6 subbands respectively. The (8; 14) translocation was shown to be reciprocal and the breaking points could be established in both chromosomes with more precision than earlier. It was noticed that the segment of No. 8, translocated onto No. 14, exhibited somewhat different morphologic features in translocated than in normal position. The concept of local prosomisation was introduced and discussed.

Acute myelogenous leukemia in a child with primary involvement of chromosomes 11 and X

“…Actually the segments involved in structural rearrangements exhibit the appearance of an earlier stage of banding differentiation, while the banding pattern of the unaffected segments of the same chromosomes display the same more condensed appearance as the rest of the chromosomes of the cell. The detailed band pattern of the 2 markers, marX/11 and marlI/X, described above is another example of local prosomization, as reported by MANOLOVA et al (1979) and . A similar effect was recently reported by HECHT and KAISERMCCAW…”

“…The fact that so many primary chromosome changes in cancer are expressed as translocations with constantly the same break points makes it reasonable to assume that a qualitative change in the nature of a mutation or a position effect is also involved. The change in chromosome phenotype called prosomi-zation, referred to above in connection with the t(X;ll) and first described in the t(8;14) of the Burkitt lymphoma (MANOLOVA et al 1979) is undoubtedly suggestive. Whatever significance the chromosome breakage may have in cancer development, it is notable that one of the break points found in 100 % of the cells of the present leukemia is located in the same band as the deletion that has a decisive (secondary?)…”

High resolution analysis of the 5q-marker chromosome in refractory anemia

“…All patients had a deletion of the long arm of chromosome 5 as revealed by conventional trypsin-Giemsa staining. Utilization of early mitotic stages for Gband analysis (MANOLOVA et al 1979) was possible in 15 patients. Ideograms of the banding patterns at the 400, 600 and 900 band stages have been constructed for each chromosome (MANOLOV and MAN- OLOVA, unpubl.).…”

Gastrointestinal Lymphoma

“…Cytogenetic and molecular genetic studies of Burkitt lymphoma have shown the presence of a reciprocal translocation involving the cMYC oncogene on chromosome 8, most commonly with the Ig heavy chain gene locus on chromosome 14 (Manolova et al, 1979). Less frequently, the translocation involves the IG chain gene loci on chromosomes 2 and 22.…”