Due to economic needs, a process of abandonment of unfavourably situated areas and intensified cultivation on favoured areas has taken place in the alpine region. This abandonment not only means the loss of a cultural landscape based on centuries of care but also interference with an established ecological balance. We wanted to investigate the impact of landuse, such as mowing, fertilisation and irrigation and the effects of abandonment on the O-horizon type, quantity and quality. Therefore, we selected areas with different types of land use but with equivalent site conditions in the Passeier Valley (Bolzano-South Tyrol province) for investigation. The areas studied were as follows: an organically fertilised and irrigated, intensively managed hay meadow mown once a year; an organically fertilised, intensively managed hay meadow mown once a year; a lightly managed hay meadow mown every second year; a lightly managed hay meadow mown every 3-4 years; an area densely covered with dwarf shrubs abandoned 10 years ago; and a reforested area abandoned 30 years ago. These selected areas represent different intensities of land use or stages of succession. On these areas, vegetation, phytomass, litter quantity, humus type, O-horizon quantity, litter decomposition and C and N storage, were analysed in detail. Abandonment causes the percentage of coverage with grasses and herbs to decrease from 100 to 10%, while lignified species increase from 0 to 90%. This leads to growth of the phytomass pools, which in turn causes an increase in the annual litter quantity (from 117 to 525 g m −2 ). Significant changes in litter quality in the case of reduced use or abandonment increases the litter's resistance to decomposition, decreasing overall decomposition. Litter quantity and quality then have a significant impact on the O-horizon quantity. This increases strongly with decreasing intensity of land use or longer times since abandonment (115-1,180 g m −2 ). This leads to a significant increase of C and N storage and a change in O-horizon type ranging from no definite O-horizon or mull O-horizons on regularly used areas to moder on abandoned areas.