Genes y moléculas implicados en la virulencia de Aspergillus fumigatus

Rementerı́a, Aitor; López-Molina, Nuria; Ludwig, Alfred; Vivanco, Ana; Bikandi, Joseba; Pontón, José; Garaizar, Javier

doi:10.1016/s1130-1406(05)70001-2

Cited by 213 publications

(61 citation statements)

References 196 publications

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“…Even though, strain Z5 was not as good as some well-studied fungi, such as A. niger MGG029 with many genomic modifications [49], but it shared a similar potential and results of the present study enable valuable resources for further explorations. The safety of this strain should be considered by knocking out some virulence-related genes, such as the genes for gliotoxin, fumagillin, aflatoxin and ribotoxin [52]. …”

Section: Discussionmentioning

confidence: 99%

Genome-wide transcriptomic analysis of a superior biomass-degrading strain of A. fumigatus revealed active lignocellulose-degrading genes

Miao

Liu

et al. 2015

BMC Genomics

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BackgroundVarious saprotrophic microorganisms, especially filamentous fungi, can efficiently degrade lignocellulose that is one of the most abundant natural materials on earth. It consists of complex carbohydrates and aromatic polymers found in the plant cell wall and thus in plant debris. Aspergillus fumigatus Z5 was isolated from compost heaps and showed highly efficient plant biomass-degradation capability.ResultsThe 29-million base-pair genome of Z5 was sequenced and 9540 protein-coding genes were predicted and annotated. Genome analysis revealed an impressive array of genes encoding cellulases, hemicellulases and pectinases involved in lignocellulosic biomass degradation. Transcriptional responses of A. fumigatus Z5 induced by sucrose, oat spelt xylan, Avicel PH-101 and rice straw were compared. There were 444, 1711 and 1386 significantly differently expressed genes in xylan, cellulose and rice straw, respectively, when compared to sucrose as a control condition.ConclusionsCombined analysis of the genomic and transcriptomic data provides a comprehensive understanding of the responding mechanisms to the most abundant natural polysaccharides in A. fumigatus. This study provides a basis for further analysis of genes shown to be highly induced in the presence of polysaccharide substrates and also the information which could prove useful for biomass degradation and heterologous protein expression.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1658-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Genome-wide transcriptomic analysis of a superior biomass-degrading strain of A. fumigatus revealed active lignocellulose-degrading genes

Miao

Liu

et al. 2015

BMC Genomics

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“…Whilst many studies have used mammalian models to investigate the impact of specific gene function on pathogenicity (Askew, 2008;Rementeria et al, 2005), there have been few studies that have compared the pathogenicity of different isolates of A. fumigatus, and those that have reported a range of pathogenicity between isolates (Jackson et al, 2009;Lionakis et al, 2005;Reeves et al, 2004 Variation in an environmental population of A. fumigatus numbers of isolates to be compared (Chamilos et al, 2010;. In A. fumigatus, these models have included Galleria larvae and Toll-deficient mutants of Drosophila (Jackson et al, 2009;Lionakis et al, 2005;Reeves et al, 2004), and relative pathogenicity has been correlated with pathogenicity levels in mammalian models (Slater et al, 2011;Chamilos et al, 2010).…”

Section: Rapd Fingerprinting Revealed Genetic Diversity In the Enviromentioning

confidence: 99%

Genetic and virulence variation in an environmental population of the opportunistic pathogen Aspergillus fumigatus

Alshareef

Robson

2014

Microbiology

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Environmental populations of the opportunistic pathogen Aspergillus fumigatus have been shown to be genotypically diverse and to contain a range of isolates with varying pathogenic potential. In this study, we combined two RAPD primers to investigate the genetic diversity of environmental isolates from Manchester collected monthly over 1 year alongside Dublin environmental isolates and clinical isolates from patients. RAPD analysis revealed a diverse genotype, but with three major clinical isolate clusters. When the pathogenicity of clinical and Dublin isolates was compared with a random selection of Manchester isolates in a Galleria mellonella larvae model, as a group, clinical isolates were significantly more pathogenic than environmental isolates. Moreover, when relative pathogenicity of individual isolates was compared, clinical isolates were the most pathogenic, Dublin isolates were the least pathogenic and Manchester isolates showed a range in pathogenicity. Overall, this suggests that the environmental population is genetically diverse, displaying a range in pathogenicity, and that the most pathogenic strains from the environment are selected during patient infection. INTRODUCTIONAspergillus fumigatus is an opportunistic fungal pathogen that can cause aspergillosis in immunocompromised patients, such as those with neutropenia or on immunosuppressive therapy following solid organ transplantation (Abad et al., 2010;Ben-Ami et al., 2010;Dagenais & Keller, 2009;Latgé, 1999;Rementeria et al., 2005). The principal route of infection is through the inhalation of airborne spores, which due to their small spore size are able to reach the alveoli (Dagenais & Keller, 2009;Ibrahim-Granet et al., 2003;Latgé, 1999;O'Gorman, 2011;Philippe et al., 2003). While other aspergilli can cause opportunistic infections (such as Aspergillus terreus and Aspergillus flavus), A. fumigatus accounts for~90 % of all cases despite airborne spore numbers only accounting for ,1 % of all Aspergillus spores (Rementeria et al., 2005). A number of studies have shown a high degree of genetic variation within the A. fumigatus population, and whilst some studies have indicated potential geographical subgroups, comparisons between clinical isolates and environmental isolates have largely been unable to distinguish these populations (Araujo et al., 2010;Aufauvre-Brown et al., 1992;Balajee et al., 2008;Chazalet et al., 1998;Denning et al., 1990;Duarte-Escalante et al., 2009; Leenders et al., 1999;Menotti et al., 2005). Moreover, epidemiological studies have largely failed to match genotypes in infected patients with genotypes found in hospitals, in part due to the high level of genetic diversity in the environmental populations (Araujo et al., 2010;Bart-Delabesse et al., 1999;Chazalet et al., 1998;Guinea et al., 2011; Leenders et al., 1999;de Valk et al., 2007), and similar findings have been reported in avian populations (Arné et al., 2011;Lair-Fulleringer et al., 2003;Olias et al., 2011). Whilst genotyping of isolates from some individual patient...

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“…This is the case of the Coccidioides-specific antigen from Coccidioides immitis and the Aspf13 antigen from Aspergillum fumigatus, both causative agents of human lung diseases (18,19), and of the immunomodulatory protein Aca1 from the medicinal fungus Antrodia camphorata (20).…”

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confidence: 99%

The Structure of the Elicitor Cerato-platanin (CP), the First Member of the CP Fungal Protein Family, Reveals a Double ψβ-Barrel Fold and Carbohydrate Binding

Oliveira

Gallo

Pazzagli

et al. 2011

Journal of Biological Chemistry

100

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Cerato-platanin (CP) is a secretion protein produced by the fungal pathogen Ceratocystis platani, the causal agent of the plane canker disease and the first member of the CP family. CP is considered a pathogen-associated molecular pattern because it induces various defense responses in the host, including production of phytoalexins and cell death. Although much is known about the properties of CP and related proteins as elicitors of plant defense mechanisms, its biochemical activity and host target(s) remain elusive. Here, we present the three-dimensional structure of CP. The protein, which exhibits a remarkable pH and thermal stability, has a double ␤-barrel fold quite similar to those found in expansins, endoglucanases, and the plant defense protein barwin. Interestingly, although CP lacks lytic activity against a variety of carbohydrates, it binds oligosaccharides. We identified the CP region responsible for binding as a shallow surface located at one side of the ␤-barrel. Chemical shift perturbation of the protein amide protons, induced by oligo-N-acetylglucosamines of various size, showed that all the residues involved in oligosaccharide binding are conserved among the members of the CP family. Overall, the results suggest that CP might be involved in polysaccharide recognition and that the double ␤-barrel fold is widespread in distantly related organisms, where it is often involved in host-microbe interactions.

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Genes y moléculas implicados en la virulencia de Aspergillus fumigatus

Cited by 213 publications

References 196 publications

Genome-wide transcriptomic analysis of a superior biomass-degrading strain of A. fumigatus revealed active lignocellulose-degrading genes

Genome-wide transcriptomic analysis of a superior biomass-degrading strain of A. fumigatus revealed active lignocellulose-degrading genes

Genetic and virulence variation in an environmental population of the opportunistic pathogen Aspergillus fumigatus

The Structure of the Elicitor Cerato-platanin (CP), the First Member of the CP Fungal Protein Family, Reveals a Double ψβ-Barrel Fold and Carbohydrate Binding

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