Genes with specific functions in the ovarian follicles of Calliphora erythrocephala (diptera)

Rubacha, Alice; Tucker, Mark A.; Valoir, T de; Belikoff, Esther J.; Beckingham, Kathy

doi:10.1016/0012-1606(88)90392-2

Cited by 7 publications

(14 citation statements)

References 37 publications

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“…Hybridization of single-strand "5S-labeled RNA probes to whole ovaries was performed as described previously (13). These probes were also used for in situ hybridization to the salivary gland polytene chromosomes (16).…”

Section: Methodsmentioning

confidence: 99%

“…Embryonic DNA was prepared as described previously (13). Subcloning in vectors Bluescribe M13' or Bluescript KS' (Stratagene), Southern hybridizations, and other DNA manipulations were performed by using standard procedures (15).…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA and poly(A)+ RNA were prepared as previously described (13). RNA was denatured in formaldehyde/formamide for electrophoresis in 1.5% agarose/formamide gels (15).…”

Section: Methodsmentioning

confidence: 99%

“…32P-labeled cDNA complementary to poly(A)+ RNA from staged Calliphora oocytes or midstage embryos was used for screening of Drosophila clone libraries as described previously (13).…”

Section: Methodsmentioning

confidence: 99%

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A second maternally expressed Drosophila gene encodes a putative RNA helicase of the "DEAD box" family.

Valoir

Tucker

Belikoff

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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Recently, a family of proteins containing the conserved motif Asp-Glu-Ala-Asp, the "DEAD box" proteins, has been identified. This family is typified by the eukaryotic translation initiation factor eIF4A, and its members are believed to share the functional property of ATP-dependent RNA unwinding. One of the previously identified members of this family (vasa) is the product of a maternally expressed gene from DrosophUa melanogaster that is known to play a role in the formation of the embryonic body plan. We report here the isolation of a Drosophila gene that has an mRNA expression pattern somewhat similar to that of vasa and also encodes a DEAD box protein. We have termed this gene ME3JB to reflect its maternal (ovarian germ-line) expression and its location within the 31B chromosome region. Comparisons with the other members of this family reveal that although ME31B is most like the protein Tifl/Tif2, which probably represents the Saccharomyces cerevisiae version of eIF4A, it is unlikely that ME31B represents the Drosophila eIF4A protein per se. A search for mutations in the ME3JB gene has established that the P element which causes the female-sterile mutation flipper lies in the 3' flank of the ME3JB gene.Recently a family of proteins that function as ATP-dependent RNA helicases has been identified (1).These proteins share a domain of about 370 amino acids in which a series of highly conserved motifs are arranged in identical order and with very similar spacing. Two of these conserved sequences represent specialized versions of the A and B motifs previously recognized in other ATP-binding proteins (2,3). The four-amino-acid sequence Asp-Glu-Ala-Asp (DEAD) is part ofthe specialized version ofthe B motif. These "DEAD box" proteins derive from organisms across all evolutionary orders (Escherichia coli to mouse), and for some of them, genetic and molecular studies have provided indications as to their functions in vivo (4-7). Thus this family of proteins appears to be involved in a wide range of intracellular events in which alteration of RNA secondary structure must play a critical role. These include formation of the initiation complex for mRNA translation, mRNA splicing, and ribosome biosynthesis. Functionally, the eukaryotic translation initiation factor eIF4A, which is the archetypal member of the family, is the one best understood at the molecular level (8-10).

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Total RNA and poly(A)+ RNA were prepared as previously described (13). RNA was denatured in formaldehyde/formamide for electrophoresis in 1.5% agarose/formamide gels (15).…”

Section: Methodsmentioning

confidence: 99%

“…32P-labeled cDNA complementary to poly(A)+ RNA from staged Calliphora oocytes or midstage embryos was used for screening of Drosophila clone libraries as described previously (13).…”

Section: Methodsmentioning

confidence: 99%

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A second maternally expressed Drosophila gene encodes a putative RNA helicase of the "DEAD box" family.

Valoir

Tucker

Belikoff

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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“…Recently, though only in a few species, a third role has been described, that is, the epithelial cells can synthesize part of the vitellogenic proteins to be incorporated in the growing oocyte (Brennan et al, 1982;Geysen et al, 1987;Rubacha et al, 1988). As the vast majority of the studies on follicle epithelial cells focuses mainly on the cells covering the oocyte, the above-mentioned functions refer only to these ones (reviews: Telfer et al, 1982;Huebner, 1984).…”

Section: Introductionmentioning

confidence: 99%

Endopolyploidy in follicle epithelial cells around the nurse chamber during oogenesis in Chrysomya putoria (Diptera, Calliphoridae)

Avancini

Mello²

1990

Genetica

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Feulgen-DNA values and nuclear areas were evaluated microspectrophotometrically for epithelial cells of the ovarian follicle during oogenesis in Chrysomya putoria. The aim was to investigate whether polyploidization occurred in the cells surrounding the nurse chamber and/or in those around the oocyte as well as whether different DNA amounts were found regarding the cell types considered. Four DNA endoreduplicative cycles could be demonstrated for the epithelial cells regardless of their localization on the follicle, during oogenesis. A small percentage of epithelial cells reaches a 32C degree. The nuclear area, however, did not increase at the same rate in cells covering the oocyte as in those covering the nurse chamber, in some of the oogenesis stages. The meaning of endopolyploidy for these cells is discussed, considering reports on relevance of secretory activities and their maintenance in polyploid cell systems.

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Invertebrate Endocrinology

Landau

Biggers

Laufer

1997

Comprehensive Physiology

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The sections in this article are: Growth and Morphogenetic Factors Insects Crustaceans Molluscs Other Invertebrates Regenerative Factors Crustaceans Insects Cnidarians Platyhelminths Annelids Echinoderms Others Pigmentary Factors Crustaceans Insects Osmoregulatory Factors Factors that May Increase Osmotic Pressure Factors that May Decrease Osmotic Pressure Metabolic Factors Biogenic Amines JHs and Farnesylacetone Adipokinetic Hormones and RPCH Crustacean Hyperglycemic Hormones Other Metabolic Factors Reproductive Factors Flatworms, Nemertines, and Nematodes Molluscs Crustaceans Insects Other Arthropods Annelids Echinoderms Behavioral Factors In Vitro Studies In Vivo Studies Pheromones Summary

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Genes with specific functions in the ovarian follicles of Calliphora erythrocephala (diptera)

Cited by 7 publications

References 37 publications

A second maternally expressed Drosophila gene encodes a putative RNA helicase of the "DEAD box" family.

A second maternally expressed Drosophila gene encodes a putative RNA helicase of the "DEAD box" family.

Endopolyploidy in follicle epithelial cells around the nurse chamber during oogenesis in Chrysomya putoria (Diptera, Calliphoridae)

Invertebrate Endocrinology

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