Genes related to osmoregulation and antioxidation play important roles in the response of Trollius chinensis seedlings to saline-alkali stress

Hou, Rongmiao; Yang, Lizhi; Wuyun, Tana; Chen, Shiyao; Zhang, Lu

doi:10.3389/fpls.2023.1080504

Cited by 14 publications

(6 citation statements)

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“…The transcription factors belonging to the C3H-type zinc finger family are crucial in various aspects of plant growth and development and in their ability to adapt to biotic and abiotic stressors ( Liu et al, 2020 ). FAR1 and NAC transcription factors families are more expressive in plants during stress conditions ( Tran et al, 2007 ; Hou et al, 2023 ). However, Srivastava et al (2018) reported the most significant members of transcription factors TIG (1,241, 10.55%), followed by FAR1 (925, 7.86%), C3H (785, 6.67%), MYB/MYB related (680, 5.78%).…”

Section: Discussionmentioning

confidence: 99%

De novo sequencing, assembly, and characterization of Asparagus racemosus transcriptome and analysis of expression profile of genes involved in the flavonoid biosynthesis pathway

Malik,

Dwivedi,

Rabuma

et al. 2023

Front. Genet.

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Asparagus racemosus is known for its diverse content of secondary metabolites, i.e., saponins, alkaloids, and a wide range of flavonoids. Flavonoids, including phenols and polyphenols, have a significant role in plant physiology and are synthesized in several tissues. Despite the diverse role of flavonoids, genetic information is limited for flavonoid biosynthesis pathways in A. racemosus. The current study explores full-scale functional genomics information of A. racemosus by de novo transcriptome sequencing using Illumina paired-end sequencing technology to elucidate the genes involved in flavonoid biosynthesis pathways. The de novo assembly of high-quality paired-end reads resulted in ∼2.3 million high-quality reads with a pooled transcript of 45,647 comprising ∼76 Mb transcriptome with a mean length (bp) of 1,674 and N50 of 1,868bp. Furthermore, the coding sequence (CDS) prediction analysis from 45,647 pooled transcripts resulted in 45,444 CDS with a total length and mean length of 76,398,686 and 1,674, respectively. The Gene Ontology (GO) analysis resulted in a high number of CDSs assigned to 25,342 GO terms, which grouped the predicted CDS into three main domains, i.e., Biological Process (19,550), Molecular Function (19,873), and Cellular Component (14,577). The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database was used to categorize 6,353 CDS into 25 distinct biological pathway categories, in which the majority of mapped CDS were shown to be related to translation (645), followed by signal transduction (532), carbohydrate metabolism (524), folding, sorting, and degradation (522). Among these, only ∼64 and 14 CDSs were found to be involved in the phenylpropanoid and flavonoid biosynthesis pathways, respectively. Quantitative Real-time PCR was used to check the expression profile of fourteen potential flavonoid biosynthesis pathway genes. The qRT-PCR analysis result matches the transcriptome sequence data validating the Illumina sequence results. Moreover, a large number of genes associated with the flavonoids biosynthesis pathway were found to be upregulated under the induction of methyl jasmonate. The present-day study on transcriptome sequence data of A. racemosus can be utilized for characterizing genes involved in flavonoid biosynthesis pathways and for functional genomics analysis in A. racemosus using the reverse genetics approach (CRISPR/Cas9 technology).

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Section: Discussionmentioning

confidence: 99%

De novo sequencing, assembly, and characterization of Asparagus racemosus transcriptome and analysis of expression profile of genes involved in the flavonoid biosynthesis pathway

Malik,

Dwivedi,

Rabuma

et al. 2023

Front. Genet.

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“…In order to maintain their water potential, plants synthesize columns of small molecules to prevent water loss, including Pro, soluble proteins, betaine, and SS [40]. Previous research has illustrated that the SS and Pro concentration in Aquilegia species [17] and Trollius chinensis [54] increased under salt or saline-alkali stress. In this study, the concentration of Pro and SS was found to be elevated in I. typhifolia under artificial saline-alkali stress (T50-T200), which showed that Pro and SS play essential roles in defending against artificial saline-alkali stress.…”

Section: Effects Of Artificial Saline-alkali Stress On the Concentrat...mentioning

confidence: 99%

Iris typhifolia Responses to Saline–Alkali Stress: Germination, Antioxidant Activity, Hormones, and Photosynthetic Performance

Chen,

Yu,

et al. 2024

Horticulturae

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Iris typhifolia Kitag is a perennial herbaceous species with high ornamental and applied value. Elucidating the mechanism of saline–alkali tolerance in Iris is crucial for their promotion in saline–alkali areas. Saline–alkali stress is one of the factors that affects plant growth, which has become a significant global issue. In this study, we measured the physiological and biochemical indexes of I. typhifolia, through germination and potting trials, to evaluate the resistance of I. typhifolia to different levels of artificial saline–alkali stress (0, 50, 100, 150, and 200 mmol·L−1). The results showed that artificial saline–alkali stress negatively impacted germination parameters, cell membrane integrity, and photosynthetic parameters. Different trends in osmoregulatory substances and endogenous hormones were observed. It was shown that I. typhifolia had a potential adaptability to the saline–alkali environment by enhancing its internal defense mechanism. Based on regression analyses, the germination threshold of I. typhifolia was calculated to be 87.15 mmol·L−1, which provided a theoretical basis for the application in soil saline–alkalization areas.

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“…It is essential to recognize that genetic factors play a pivotal role in determining how a plant responds to stressors. Hou et al (2023) reported that genes related to osmoregulation and antioxidation play important roles in the response of Trollius chinensis. Some genotypes may exhibit higher resilience and adaptive capacity, leading to nonsignificant differences at specific doses, while others may be more susceptible, resulting in significant variations in leaf morphology.…”

Section: Morphological Leaf Characteristics Of Pennisetum Purpureum C...mentioning

confidence: 99%

Radiosensitivity Assessment and the Impact of Gamma Radiation on the Growth and Diversity of Pennisetum purpureum Cv Mot in Marginal Land

Putra,

Prasetya

2024

American Journal of Animal and Veterinary Sciences

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This study aims to assess the radiosensitivity of Pennisetum purpureum cv Mott, with the primary objectives of determining the LD50 value, evaluating the effects of gamma radiation doses on plant growth and diversity, and elucidating the specific goals of irradiation in relation to the overall research. The research encompasses a wide range of radiation doses, ranging from 0-400 Gy, including multiple double doses (×2 Gy). The investigation involved irradiating Pennisetum purpureum cv Mott seeds (cuttings) and observing their seed germination capacity after one month. This research was conducted with rigorous scientific methodology, involving five replicates, each consisting of 100 plants. The study was carried out in marginal land conditions with a pH range of 4.5-5. These specific experimental conditions were chosen to simulate real-world scenarios and provide valuable insights into the radiosensitivity of Pennisetum purpureum cv Mott under conditions similar to those found in marginal agricultural areas. It is noteworthy that this study represents a novel contribution to the field of radiosensitivity in plants, as it differs significantly from previous research endeavors. This research offers a fresh perspective and distinctive findings that set it apart from prior studies. In addressing ethical standards, it is essential to note that this study strictly adhered to ethical considerations, particularly regarding the use of radiation on plants. All procedures were carried out in accordance with established ethical guidelines and compliance with radiation safety protocols. The findings reveal that radiation doses significantly influence seed germination and the morphology of leaves, stems, and roots in Pennisetum purpureum cv Mott. The study indicates that the Lethal Dose 50 (LD50) for Pennisetum purpureum cv Mott is approximately 28.79 Gy. Furthermore, a diverse range of traits, including leaf count, leaf length, plant height, stem length, and stem diameter, exhibit varying degrees of heritability, with coefficients of genetic variation (KKG) ranging from low to very high. In conclusion, this comprehensive and novel study sheds light on the impact of gamma radiation doses on Pennisetum purpureum cv Mott. The results are instrumental in understanding the radiosensitivity of this plant species and hold potential applications in agronomy, especially in marginal land cultivation. Furthermore, this research contributes to a deeper understanding of radiation effects on plants, specifically within the context of Pennisetum purpureum cv Mott in marginal land conditions while adhering to ethical standards and introducing novel insights into the field.

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Genes related to osmoregulation and antioxidation play important roles in the response of Trollius chinensis seedlings to saline-alkali stress

Cited by 14 publications

References 58 publications

De novo sequencing, assembly, and characterization of Asparagus racemosus transcriptome and analysis of expression profile of genes involved in the flavonoid biosynthesis pathway

De novo sequencing, assembly, and characterization of Asparagus racemosus transcriptome and analysis of expression profile of genes involved in the flavonoid biosynthesis pathway

Iris typhifolia Responses to Saline–Alkali Stress: Germination, Antioxidant Activity, Hormones, and Photosynthetic Performance

Radiosensitivity Assessment and the Impact of Gamma Radiation on the Growth and Diversity of Pennisetum purpureum Cv Mot in Marginal Land

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