Genes involved in virulence of the entomopathogenic fungus Beauveria bassiana

Valero-Jiménez, Claudio A.; Wiegers, Harm; Zwaan, Bas J.; Koenraadt, Constantianus J. M.; Kan, Jan A.L. van

doi:10.1016/j.jip.2015.11.011

Cited by 108 publications

(81 citation statements)

References 87 publications

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“…Isolates of B. bassiana and M. anisopliae s.l. exhibit high genetic and virulence diversity [27, [36][37][38][39][40]. Comparative genomic analyses for both genera have revealed qualitative differences in gene content that are potentially important in determining host virulence [41][42][43][44][45].…”

Section: Insect Pathogensmentioning

confidence: 99%

Managing the plant microbiome for biocontrol fungi: examples from Hypocreales

Kepler

Maul

Rehner³

2017

Current Opinion in Microbiology

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Section: Insect Pathogensmentioning

confidence: 99%

Managing the plant microbiome for biocontrol fungi: examples from Hypocreales

Kepler

Maul

Rehner³

2017

Current Opinion in Microbiology

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“…Similarly, the genetic identity to respect to Bb8028 was higher in Bb5078 (90.4%) and Bb2597 (89.7%), and lower in Bb4305 (88.4%) and Bb1520 (87.9%). We searched for SNPs in coding sequences of a set of 54 genes that have been functionally analysed by knock-out mutagenesis (reviewed in [15]) between the five B. bassiana isolates sequenced in this study. There are two genes that contain a SNP with potentially high impact: in the α-glucoside transporter gene Bbagt 1 in a single isolate, and in the thioredoxin gene trx 3 in two isolates.…”

Section: Resultsmentioning

confidence: 99%

“…Despite all efforts made to understand virulence in B. bassiana (for a review, see [15]), many of the genes that were functionally analysed thus far are involved in general biological processes (e.g., conidiation, stress response) that pleiotropically affect virulence. This indicates that virulence is tightly connected to the biology and life history of Beauveria .…”

Section: Introductionmentioning

confidence: 99%

Comparative genomics of Beauveria bassiana: uncovering signatures of virulence against mosquitoes

et al. 2016

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BackgroundEntomopathogenic fungi such as Beauveria bassiana are promising biological agents for control of malaria mosquitoes. Indeed, infection with B. bassiana reduces the lifespan of mosquitoes in the laboratory and in the field. Natural isolates of B. bassiana show up to 10-fold differences in virulence between the most and the least virulent isolate. In this study, we sequenced the genomes of five isolates representing the extremes of low/high virulence and three RNA libraries, and applied a genome comparison approach to uncover genetic mechanisms underpinning virulence.ResultsA high-quality, near-complete genome assembly was achieved for the highly virulent isolate Bb8028, which was compared to the assemblies of the four other isolates. Whole genome analysis showed a high level of genetic diversity between the five isolates (2.85–16.8 SNPs/kb), which grouped into two distinct phylogenetic clusters. Mating type gene analysis revealed the presence of either the MAT1–1–1 or the MAT1–2–1 gene. Moreover, a putative new MAT gene (MAT1-2–8) was detected in the MAT1–2 locus. Comparative genome analysis revealed that Bb8028 contains 163 genes exclusive for this isolate. These unique genes have a tendency to cluster in the genome and to be often located near the telomeres. Among the genes unique to Bb8028 are a Non-Ribosomal Peptide Synthetase (NRPS) secondary metabolite gene cluster, a polyketide synthase (PKS) gene, and five genes with homology to bacterial toxins. A survey of candidate virulence genes for B. bassiana is presented.ConclusionsOur results indicate several genes and molecular processes that may underpin virulence towards mosquitoes. Thus, the genome sequences of five isolates of B. bassiana provide a better understanding of the natural variation in virulence and will offer a major resource for future research on this important biological control agent.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3339-1) contains supplementary material, which is available to authorized users.

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“…Selection of a suitable incubation time is criti-doi: 10.17221/110/2015-PPS cal to a bioassay procedure taking into consideration the infection cycle of the tested fungus. Many factors enter the host-pathogen interaction and influence the progress of infection cycle (Valero-Jiménez et al 2016). Besides environmental factors, like temperature and humidity, also the host's fitness and size, as well as the number of infection particles (a dose of conidia) that get in contact with host's cuticle should be taken into consideration.…”

Section: Discussionmentioning

confidence: 99%

“…The fungi can spread fast among host populations horizontally via aerially produced conidia and infect their host by penetrating the cuticle with germ hyphae (Valero-Jiménez et al 2016). Species spectrum and prevalence of these fungi in the leaf miner populations and their effect on the host populations have been only marginally studied (e.g.…”

mentioning

confidence: 99%

Natural prevalence of entomopathogenic fungi in hibernating pupae of Cameraria ohridella (Lepidoptera: Gracillariidae) and virulence of selected isolates

Schemmer¹,

Chládeková²,

Medo³

et al. 2016

Plant Prot. Sci.

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Schemmer R., Chládeková P., Medo J., Barta M. (2016): Natural prevalence of entomopathogenic fungi in hibernating pupae of Cameraria ohridella (Lepidoptera: Gracillariidae) and virulence of selected isolates. Plant Protect. Sci., Spontaneous infection of hibernating Cameraria ohridella pupae by entomopathogenic fungi was monitored at two localities of south-western Slovakia and efficacy of selected fungal isolates against the pupae was evaluated in laboratory. Natural prevalence of fungal infection in pupal populations was low (< 7%) and varied depending upon collecting date, locality, year, and pathogen. Ten isolates of three fungal species, Beauveria pseudobassiana, Isaria fumosorosea, and I. farinosa, isolated from the naturally infected pupae and three isolates of non-insect origin of two fungi, B. bassiana and metarhizium anisopliae, were screened for colony growth, conidial production in vitro, and virulence to C. ohridella pupae. A significant variability in the evaluated traits was detected among the isolates. Pupae demonstrated vulnerability to all the isolates irrespective of their origin, however virulence of isolates varied significantly. I. fumosorosea was the most virulent fungus with median lethal concentration of 0.09 × 10 4 conidia/ml (isolate CO10-IFu) and mean survival time of pupae of 7.14 days (isolate CO8-IFu). No correlation between conidial yield of isolates and fungal virulence was observed, but a moderately strong relationship was detected between virulence and mycelial growth rate of isolates.

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Genes involved in virulence of the entomopathogenic fungus Beauveria bassiana

Cited by 108 publications

References 87 publications

Managing the plant microbiome for biocontrol fungi: examples from Hypocreales

Managing the plant microbiome for biocontrol fungi: examples from Hypocreales

Comparative genomics of Beauveria bassiana: uncovering signatures of virulence against mosquitoes

Natural prevalence of entomopathogenic fungi in hibernating pupae of Cameraria ohridella (Lepidoptera: Gracillariidae) and virulence of selected isolates

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