Genes involved in the establishment of hepatic steatosis in Muscovy, Pekin and mule ducks

Tavernier, Annabelle; Davail, Stéphane; Ricaud, Karine; Bernadet, Marie-Dominique; Gontier, Karine

doi:10.1007/s11010-016-2850-7

Cited by 22 publications

(34 citation statements)

References 31 publications

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“…Overfeeding increased liver weight and fattening levels in the control group, in line with previous studies [ 8 , 9 , 14 , 60 ]. However, while the body weight was affected by both probiotic supplementations during the first 28 days, other growth parameters (liver, fat and muscle weights) were not improved.…”

Section: Discussionsupporting

confidence: 91%

“…Next, the plasma concentrations of glucose and triglycerides (TG) increased between SP and Eof point, which is quite consistent with the metabolic state of overfed ducks and geese, where significant changes occur [ 13 , 14 , 68 , 69 ]. So as previously described in these studies, the overfeeding period strongly increased the TG and glucose plasma concentration in our study as well.…”

Section: Discussionmentioning

confidence: 52%

“…Previous studies have also shown that the diversity of intestinal microbiota is modified by diet [ 12 ]. During overfeeding, waterfowls are exclusively fed with corn which induces a hepatic steatosis called “foie gras”, resulting from the storage of fatty acids in the liver [ 13 , 14 ]. Recent studies have shown a strong effect of diet and genetics on microbiota diversity, in particular an increase in Lactobacillus spp.…”

Section: Introductionmentioning

confidence: 99%

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Probiotics Strains Modulate Gut Microbiota and Lipid Metabolism in Mule Ducks

Even¹,

Davail²,

Rey³

et al. 2018

TOMICROJ

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Background:Livestock production should respond to societal, environmental and economic changes. Since 2006 and the ban on antibiotics as growth factors in European Union, the use of probiotics has become widespread and has demonstrated the effect of intestinal microbiota on the performance of farm animals.Objective:The aim of this study was to investigate the effect of supplementation with Lactobacillus salivarius (as a probiotics strain or combined with other strains) on zootechnical performance, metabolic and immune gene expression and intestinal microbiota diversity in mule ducks using high-throughput sequencing and real-time PCR.Method:The mule ducks were reared for 79 days and overfed for 12 days with or without probiotics. Samples were collected at 14 (starting period) and 91 days (end of overfeeding period), 3 hours post feeding.Results:Irrespective of digestive content, age, level of feed intake or supplementation with probiotics, Firmicutes, Proteobacteria and Bacteroidetes were the dominant phyla in the bacterial community in mule ducks. At 14 days, both the ileal and cecal samples were dominated by Firmicutes (in particular the Clostridiales order). Overfeeding induced a shift between Clostridiales and Lactobacillales in the ileal samples whereas in the cecal samples, the relative abundance of Firmicutes decreased. Overfeeding also induced hepatic over-expression of Fatty Acid Synthase (FAS) and of the lipid transporter gene Fatty Acid Binding Protein 4 (FABP4). This increase in lipid metabolism genes is associated with a decrease in inflammatory response.Conclusion:Finally, probiotic supplementation had only a slight impact on gene expression and microbiota diversity, both at 14 days and after overfeeding.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

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Probiotics Strains Modulate Gut Microbiota and Lipid Metabolism in Mule Ducks

Even¹,

Davail²,

Rey³

et al. 2018

TOMICROJ

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“…Second important conclusion is that many genes are differentially expressed in the four genetic types. This suggests that response to overfeeding and consequently development of hepatic steatosis is a complex trait involving many genes and functions and is not well described by the expression of few candidate genes [6][7][8][9], even if expressions of these genes are well correlated to fatty liver weight [6].…”

Section: Discussionmentioning

confidence: 99%

De novo transcriptome assembly and hepatic gene expression analyses in overfed and ad libitum fed Muscovy and Pekin ducks and their reciprocal inter-specific Mule and Hinny hybrids

Liu

Herault

Diot

et al. 2020

Preprint

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Background Common Pekin and Muscovy ducks and their intergeneric hinny and mule hybrids have different abilities for fatty liver production. RNA-Seq analyses from the liver of these different genetic types fed ad libitum or overfed would help to identify genes with different response to overfeeding between them. However RNA-seq analyses from different species and comparison is challenging. The goal of this study was develop a relevant strategy for transcriptome analysis and comparison between different species. Results Transcriptomes were first assembled with a reference-based approach. Important mapping biases were observed when heterologous mapping were conducted on common duck reference genome, suggesting that this reference-based strategy was not suited to compare the four different genetic types. De novo transcriptome assemblies were then performed using Trinity and Oases. Assemblies of transcriptomes were not relevant when more than a single genetic type was considered. Finally, single genetic type transcriptomes were assembled with DRAP in a mega-transcriptome. No bias was observed when reads from the different genetic types were mapped on this mega-transcriptome and differences in gene expression between the four genetic types could be identified. Conclusions Analyses using both reference-based and de novo transcriptome assemblies point out a good performance of the de novo approach for the analysis of gene expression in different species. It also allowed the identification of differences in responses to overfeeding between Pekin and Muscovy ducks and hinny and mule hybrids.

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“…However, they mainly focused on biochemical levels [1][2][3][4][5]. Some studies also focused on gene expression levels but were conducted on few candidate genes only [6][7][8][9]. This was mainly due to the lack of duck speci c microarrays for transcriptome analyses at the genome level.…”

Section: Introductionmentioning

confidence: 99%

Development of a relevant strategy using de novo transcriptome assembly method for transcriptome comparisons between Muscovy and common duck species and their reciprocal inter-specific Mule and Hinny hybrids fed ad libitum and overfed

Liu

Herault

Diot

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Common Pekin and Muscovy ducks and their intergeneric hinny and mule hybrids have different abilities for fatty liver production. RNA-Seq analyses from the liver of these different genetic types fed ad libitum or overfed would help to identify genes with different response to overfeeding between them. However RNA-seq analyses from different species and comparison is challenging. The goal of this study was develop a relevant strategy for transcriptome analysis and comparison between different species.Results: Transcriptomes were first assembled with a reference-based approach. Important mapping biases were observed when heterologous mapping were conducted on common duck reference genome, suggesting that this reference-based strategy was not suited to compare the four different genetic types. De novo transcriptome assemblies were then performed using Trinity and Oases. Assemblies of transcriptomes were not relevant when more than a single genetic type was considered. Finally, single genetic type transcriptomes were assembled with DRAP in a mega-transcriptome. No bias was observed when reads from the different genetic types were mapped on this mega-transcriptome and differences in gene expression between the four genetic types could be identified.Conclusions: Analyses using both reference-based and de novo transcriptome assemblies point out a good performance of the de novo approach for the analysis of gene expression in different species. It also allowed the identification of differences in responses to overfeeding between Pekin and Muscovy ducks and hinny and mule hybrids.

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Genes involved in the establishment of hepatic steatosis in Muscovy, Pekin and mule ducks

Cited by 22 publications

References 31 publications

Probiotics Strains Modulate Gut Microbiota and Lipid Metabolism in Mule Ducks

Probiotics Strains Modulate Gut Microbiota and Lipid Metabolism in Mule Ducks

De novo transcriptome assembly and hepatic gene expression analyses in overfed and ad libitum fed Muscovy and Pekin ducks and their reciprocal inter-specific Mule and Hinny hybrids

Development of a relevant strategy using de novo transcriptome assembly method for transcriptome comparisons between Muscovy and common duck species and their reciprocal inter-specific Mule and Hinny hybrids fed ad libitum and overfed

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