Genes involved in sex differentiation, epigenetic reprogramming, and cell fate regulate sex change in a wrasse

Muncaster, Simon; Goikoetxea, Alexander; Lokman, P. Mark; Moraes, C. E. De Farias e; Damsteegt, Erin L.; Edgecombe, Jonika; Gemmell, Neil J.; Todd, Erica V.

doi:10.1007/s11160-022-09755-2

Cited by 2 publications

(3 citation statements)

References 72 publications

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“…Correspondingly, RSPO1 has been reported to be highly expressed in the female ovaries of P. sinensis (Liang, Meng, et al, 2019b). This study, however, revealed a surprising female‐biased expression of PsZNRF3 , with significantly higher expression levels in ovaries than in testes, diverging from mammalian patterns yet aligning with findings in Neocaridina denticulate (Harris et al, 2018; Muncaster et al, 2023; Rjiba et al, 2023). Additionally, the study compared expression levels (FPKM values) of PsZNRF3 across different embryonic development stages between females and males, revealing consistently higher expression in females.…”

Section: Discussionsupporting

confidence: 81%

“…Moreover, Mu et al (2015) utilized molecular cytogenetics and cross‐population incubation experiments to demonstrate that P. sinensis does not adhere to TSD. The latest research showed that socially induced sex change is orchestrated by a novel combination of genes and epigenetic factors that govern sex differentiation and cell fate (Muncaster et al, 2023). This hypothesis could extend to P. sinensis , where the sex determination system might be governed by both environmental conditions and genetic factors.…”

Section: Introductionmentioning

confidence: 99%

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Whole‐genome resequencing reveals novel sex‐related markers and candidate gene in the Chinese soft‐shelled turtle (Pelodiscus sinensis)

Zeng,

Chen,

Zeng

et al. 2024

J World Aquaculture Soc

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An apparent sexual dimorphism, such as growth rate and immunocompetence, is observed in the Chinese soft‐shelled turtle (Pelodiscus sinensis). The identification of sex‐related markers and candidate genes holds significance for the sex‐controlled breeding of P. sinensis. In this study, through an integration of whole‐genome resequencing and Sanger sequencing, 21 female‐specific single nucleotide polymorphisms (SNPs) were identified. Based on these SNPs, the rapid and cost‐effective molecular methodologies for genetic sex identification in P. sinensis were established. Of note, 19 validated SNPs are located within the zinc and ring finger 3 (ZNRF3) gene, which has been reported to be testis‐determining in mammals. Cloning of the ZNRF3 gene from P. sinensis (PsZNRF3) and analysis of its expression profile in tissues and in response to sex steroid hormone treatment demonstrated exhibiting a female‐biased expression pattern. These findings not only provide molecular markers for sex‐controlled breeding in P. sinensis but also open up a solid basis for revealing the sex‐determination mechanism of this species.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Whole‐genome resequencing reveals novel sex‐related markers and candidate gene in the Chinese soft‐shelled turtle (Pelodiscus sinensis)

Zeng,

Chen,

Zeng

et al. 2024

J World Aquaculture Soc

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“…However, the expression pattern of rspo1 in fish seems not very conservative. For instance, in many teleost fishes such as zebrafish, medaka, and half-smooth tongue, rspo1 shows a female bias, while in some ancient fishes (like Acipenser stephensi , coelacanth, and lungfish) and some sex-changed teleost fishes (like Notolabrus celidotus ) shows a male bias( Biscotti et al, 2018 ; Zhang et al, 2020 ; Muncaster et al, 2023 ), implying that rspo1 may have other gender-related functions in various fishes.…”

Section: Discussionmentioning

confidence: 99%

Gonadal transcriptome sequencing reveals sexual dimorphism in expression profiling of sex-related genes in Asian arowana (Scleropages formosus)

Zhao,

Bian,

et al. 2024

Front. Genet.

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Asia arowana (Scleropages formosus) is an ornamental fish with high economic value, while its sex determination mechanism is still poorly understood. By far, no morphological evidence or molecular marker has been developed for effective distinguishment of genders, which poses a critical challenge to our captive breeding efforts. In this study, we sequenced gonadal transcriptomes of adult Asian arowanas and revealed differential expression profiling of sex-related genes. Based on the comparative transcriptomics analysis of testes (n = 3) and ovaries (n = 3), we identified a total of 8,872 differentially expressed genes (DEGs) and 18,490 differentially expressed transposable elements (TEs) between male and female individuals. Interestingly, the expression of TEs usually has been more significantly testis-biased than related coding genes. As expected, several genes related to females (such as foxl2 and cyp19a1a) are significantly transcribed in the ovary, and some genes related to male gonad development (such as dmrt1, gsdf and amh) are highly expressed in the testis. This sexual dimorphism is valuable for ascertaining the differential expression patterns of sex-related genes and enriching the genetic resources of this economically important species. These valuable genetic materials thereby provide instructive references for gender identification and one-to-one breeding practices so as to expand fish numbers for a rapid elevation of economic value.

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Genes involved in sex differentiation, epigenetic reprogramming, and cell fate regulate sex change in a wrasse

Cited by 2 publications

References 72 publications

Whole‐genome resequencing reveals novel sex‐related markers and candidate gene in the Chinese soft‐shelled turtle (Pelodiscus sinensis)

Whole‐genome resequencing reveals novel sex‐related markers and candidate gene in the Chinese soft‐shelled turtle (Pelodiscus sinensis)

Gonadal transcriptome sequencing reveals sexual dimorphism in expression profiling of sex-related genes in Asian arowana (Scleropages formosus)

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