Genes Ia, II, III, IV and V of Soybean chlorotic mottle virus are essential but the gene Ib product is non-essential for systemic infection

Takemoto, Yutaka; Hibi, Tadaaki

doi:10.1099/0022-1317-82-6-1481

Cited by 10 publications

(3 citation statements)

References 46 publications

(69 reference statements)

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“…However, CmYLCV differs from the two other members of the SbCMV-like genus in the absence of the nonessential ORF VII (Mushegian et al, 1995;Takemoto & Hibi, 2001). The lack of ORF VII and the exclusion of the tRNA Met binding site from ORF Ib, the other dispensable gene in the genome, could represent an evolutionary strategy of CmYLCV to increase efficiency.…”

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confidence: 98%

“…Indeed, the SbCMV-like genus is characterized by the presence of three ORFs between ORF I and ORF IV instead of the two typical of CaMV. The additional ORF ('Ib' in SbCMV and 'A' in PCSV) following gene I contains the tRNA Met binding site but, excluding this domain, it is dispensable for virus infectivity (Mushegian et al, 1995;Takemoto & Hibi, 2001). In CmYLCV, ORF I terminates the larger intergenic region and is followed by a small ORF Ib (Fig.…”

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confidence: 99%

“…2b) and hence probably also protein function. Although there is some indication for the absence of a shunting mechanism in SbCMV (Takemoto & Hibi, 2001) and CmYLCV, a possible alternative strategy of gene expression involving TAV-mediated reinitiation of translation (Park et al, 2001) is not excluded.…”

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confidence: 99%

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Characterization of Cestrum yellow leaf curling virus: a new member of the family Caulimoviridae

Stavolone

Ragozzino

Höhn

2003

Journal of General Virology

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Cestrum yellow leaf curling virus (CmYLCV) has been characterized as the aetiological agent of the Cestrum parqui mosaic disease. The virus genome was cloned and the clone was proven to be infectious to C. parqui. The presence of typical viroplasms in virus-infected plant tissue and the information obtained from the complete genomic sequence confirmed CmYLCV as a member of the Caulimoviridae family. All characteristic domains conserved in plant pararetroviruses were found in CmYLCV. Its genome is 8253 bp long and contains seven open reading frames (ORFs). Phylogenetic analysis of the relationships with other members of the Caulimoviridae revealed that CmYLCV is closely related to the Soybean chlorotic mottle virus (SbCMV)-like genus and particularly to SbCMV. However, in contrast to the other members of this genus, the primer-binding site is located in the intercistronic region following ORF Ib rather than within this ORF, and an ORF corresponding to ORF VII is missing.

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confidence: 99%